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RECOMBINANT BOVINE SERUM ALBUMIN MATURE PEPTIDE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
RECOMBINANT BOVINE SERUM ALBUMIN MATURE PEPTIDE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
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机译:重组牛血清白蛋白成熟肽,其制备方法及其应用
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摘要
A recombinant bovine serum albumin mature peptide, having an amino acid residue sequence as shown in SEQ ID NO: 1, and an optimized nucleotide sequence encoding the recombinant bovine serum albumin mature peptide as shown in SEQ ID NO: 3. A preparation method for the recombinant bovine serum albumin mature peptide comprises: synthesizing an optimized gene of a bovine serum albumin mature peptide; then integrating the gene into an expression vector, and using the vector to transform various host cells for expression; culturing the transformed host cells to obtain a fermentation broth containing a large amount of a recombinant protein; and further separating and purifying the fermentation broth to give the product. Under mass spectrometry the above recombinant bovine serum albumin is completely consistent with a natural bovine serum albumin mature peptide sequence, has the advantage of being free from animal virus contamination and has high purity, is a natural bovine serum albumin substitute, and can be applied in biochemical pharmaceuticals and other industries.
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机译:一种重组牛血清白蛋白成熟肽,其具有如SEQ ID NO:1所示的氨基酸残基序列,以及编码如SEQ ID NO:3所示的重组牛血清白蛋白成熟肽的优化的核苷酸序列。重组牛血清白蛋白成熟肽包括:合成优化的牛血清白蛋白成熟肽基因;然后将该基因整合到表达载体中,并使用该载体转化各种宿主细胞进行表达。培养转化的宿主细胞以获得含有大量重组蛋白的发酵液;进一步分离纯化发酵液得到产物。在质谱分析下,上述重组牛血清白蛋白与天然牛血清白蛋白成熟肽序列完全一致,具有不受动物病毒污染且纯度高的优点,是天然牛血清白蛋白的替代品,可用于生化制药等行业。
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