METHOD FOR THE DIAGNOSIS OF C3NEF-MEDIATED MEMBRANOPROLIFERATIVE GLOMERULONEPHRITIS

摘要

A method for the diagnosis of C3NeF-mediated membranoproliferative glomerulonephritis (MPGN) in a sample comprising: a) immobilization of C3b in at least two wells of a plate; b) formation in said wells of the C3bBb complex, and, optionally, denaturation of the same complex, optionally with the addition of Factor H (FH), wherein said formation of the complex is mediated by the addition of IgGs purified from said sample and/or control in the presence of Factor B (FB), Factor D (FD), bovine serum albumin (BSA), magnesium ions; d) detachment of the formed complexes; e) electrophoretic run under denaturing conditions of said complexes and transfer on a membrane; detection of the protein of interest, Bb, which is one of the two components of C3 convertase; wherein the ratio of the intensities measured for the Bb band in the samples collected by each of said wells is indicative of C3NeF activity in said sample.