109 KNOCK-IN MOUSE HOMOLOGOUS RECOMBINATED BY BANK VOLE'S PRION PROTEIN GENE WHICH 109TH AMINO ACID IS ISOLEUCINE AND APPLICATIONS THEREOF

摘要

The present invention relates to a knock-in mouse homologous recombinated with a bank vole′s prion protein gene in which the 109th amino acid is isoleucine; and an application of the same. More specifically, the present invention relates to a knock-in mouse whose inherent prion protein gene is knocked-in with a cellular prion protein gene of a bank vole in which the 109th amino acid is isoleucine, by a homologous recombination method; and an application of the same. According to the present invention, a knock-in mouse may be produced whose inherent prion protein gene is replaced (substituted) with the gene of the prion protein of a bank vole in which the 109th amino acid is isoleucine by a homologous recombination method, and the knock-in mouse thus produced may be used beneficially as an animal model used to evaluate the anti-prion validity of a candidate material of a drug developed to treat prion disease including CJD. Further, the present invention may be beneficially used in studies to identify a role in the emergence of prions or to improve the performance of an anti-prion material.