FVII EGF2-Catalytic domain Manufacturing method of skin functional peptide group derived from recombinant EGF2-Catalytic domain of blood coagulation factor FVII

摘要

The present invention relates to a method of fragmenting a recombinant EGF2-catalytic domain using chemical treatment and proteolytic enzymes, after constructing an expression vector of the EGF2-catalytic domain gene of the blood coagulation factor FVII, expressing proteins thereof, and purifying the recombinant EGF2-catalytic domain with high purity through a chromatography system. In addition, peptides derived from each fragment of the EGF2-catalytic domain have exhibited protein activity in the cell activation effect and collagen biosynthesis promoting effect.