Pneumococcal vaccine combining selected alpha-helix domains and proline-rich domains of pneumococcal surface protein A

摘要

This embodiment provides compositions and methods related to novel recombinant protein immunogens comprising specific portions of the proline rich region (PRD) and alpha helix domain (aHD) of pneumococcal surface protein A (PspA), the portions Connected to provide an aHD-PRD construct. The aHD and PRD proteins that make up the aHD-PRD construct are selected to maximize cross-reactivity and provide protection against a wide range of pneumococcal serotypes. In addition, an immunogenic composition comprising at least one aHD-PRD construct, including a vaccine, may comprise an unlinked aHD moiety. In addition, recombinant nucleic acid molecules encoding aHD-PRD constructs, vectors containing such molecules and recombinant host cells, aHD-PRD expression products, the use of such nucleic acid molecules for expressing aHD-PRD constructs by recombinant techniques, and suitable The use of expression products to elicit an immune or protective response to pneumococcal disease in a host is provided.