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Method for detection of antigen using fluorescence resonance energy transfer immunoassay on membrane

机译:膜上荧光共振能量转移免疫法检测抗原的方法

摘要

The method for detecting an antigen according to an embodiment of the present invention comprises the steps of (a) contacting a sample containing an antigen to be detected with the other end of a diagnostic unit formed of a membrane containing an antibody against the antigen to be detected, Process; (b) irradiating the antigen-antibody complex formed by the step (a) with light to cause fluorescence resonance energy transfer and obtaining a spectrum therefrom; And (c) analyzing the spectrum obtained in the step (b) to determine the presence or absence of the antigen to be detected and measuring the concentration.
机译:根据本发明的一个实施方案的用于检测抗原的方法包括以下步骤:(a)使包含待检测抗原的样品与由含有针对待抗原的抗体的膜形成的诊断单元的另一端接触。检测到,过程; (b)用光照射步骤(a)形成的抗原-抗体复合物以引起荧光共振能量转移并从中获得光谱;并且(c)分析在步骤(b)中获得的光谱以确定是否存在要检测的抗原并测量浓度。

著录项

  • 公开/公告号KR101945997B1

    专利类型

  • 公开/公告日2019-02-08

    原文格式PDF

  • 申请/专利权人 광주과학기술원;

    申请/专利号KR20160060797

  • 发明设计人 김민곤;오현경;

    申请日2016-05-18

  • 分类号G01N33/53;G01N21/64;G01N33/558;G01N33/68;

  • 国家 KR

  • 入库时间 2022-08-21 11:49:13

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