FIELD: biotechnology.;SUBSTANCE: invention refers to biotechnology, molecular biology and medical microbiology. A set of oligonucleotide primers for the loop isothermal amplification reaction (LAMP), which enables to amplify bacteria-specific Francisella tularensis fragments of DNA from the target gene having the nucleotide sequence SEQ ID No. 1. For amplification of specific DNA fragments of Francisella tularensis in the loop isothermal amplification reaction, a set of oligonucleotide primers is proposed, which includes: a FIP primer – "acpFt101-F3", having a nucleotide sequence SEQ ID No. 2, primer BIP – "acpFt101-B3", having nucleotide sequence SEQ ID No. 3, primer F3 – "acpFt101-FIP", having nucleotide sequence SEQ ID No. 4, primer B3 – "acpFt101-BIP" having nucleotide sequence SEQ ID No. 5.;EFFECT: declared invention allows detecting presence of tularemia pathogen – Francisella tularensis within 1–2 hours with high specificity in samples of pure cultures in concentrations from 10 to 1,000 m.c. in ml (depending on strains).;3 cl, 4 dwg, 1 tbl, 3 ex
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机译：技术领域：发明：涉及生物技术，分子生物学和医学微生物学。一套用于环等温扩增反应（LAMP）的寡核苷酸引物，它能够从具有核苷酸序列SEQ ID No.1的靶基因中扩增细菌特异性的土拉弗朗西斯菌DNA片段。在回路等温扩增反应中，提出了一套寡核苷酸引物，包括：FIP引物–具有核苷酸序列SEQ ID No. 2的“ acpFt101-F3”，引物BIP –具有核苷酸序列的引物BIP –“ acpFt101-B3” SEQ ID No.3，引物F3 –“ acpFt101-FIP”，具有核苷酸序列SEQ ID No. 4，引物B3 –“ acpFt101-BIP”，具有核苷酸序列SEQ ID No. 5; tularemia病原体– 1-2小时内出现了tularensis弗朗西斯菌，对浓度从10到1,000 mc的纯培养物样品具有高度特异性单位：ml（取决于菌株）。;3 cl，4 dwg，1 tbl，3 ex
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