THREE-DIMENSIONAL RECONSTRUCTION VISUAL INTEGRATION METHOD FOR HUMAN BODY PERIPHERAL NERVE INTERNAL TRACT TYPE STRUCTURE

摘要

The present invention relates to fields of clinical application of nerve defect repair and the medical three-dimensional (3D) printing technology, and provides an integrated visualization method for three-dimensional (3D) reconstruction of internal structure of human peripheral nerves. The method comprises the following steps: obtaining human peripheral nerves, preparing nerve specimens ex vivo by staining with an iodine preparation in combination with a freeze-drying method; scanning the pretreated peripheral nerves using Micro CT to acquire two-dimensional images, and performing binarization processing to the two-dimensional images, then conducting image segmentation based on textural features to acquire images of nerve fascicles; finally, reconstructing the segmented images into a visualization model by using a supercomputer. The method of capturing images in the invention enables the scanning accuracy to meet the requirements of reconstructing nerve fascicles; the visualization model can provide stereoscopic anatomical atlas for achievement of interfascicular nerve anastomosis in clinical practice; simultaneously, the obtained three-dimensional data can be used to create templates for bio-manufactured neurobiological materials to achieve a goal of precise repair.