BROTH MICRODILUTION METHOD FOR EVALUATING AND DETERMINING MINIMAL INHIBITORY CONCENTRATION OF ANTIBACTERIAL POLYPEPTIDES

摘要

The present invention provides components, assays and methods for evaluating antibacterial effectiveness and determining minimal inhibitory concentration (MIC) of polypeptides, including lysin polypeptides, that kill bacteria. Modified broth microdilution components and methods are provided, including supplements to enable accurate MIC determination mimicking lysin polypeptide activity in human matrices including serum and blood.