METHOD FOR DETECTING AND QUANTIFYING VITAMINS AND THYROID ANALYTES FROM A URINE SAMPLE

摘要

A method for analyzing a urine sample includes binding a plurality of analytes from a urine sample to an adsorbent; and analyzing the plurality of analytes using a molecular analyzer to detect evidence of at least one water soluble vitamin and at least one fat soluble vitamin in the urine sample. The method can also include analyzing the urine sample using the molecular analyzer to detect a thyroid analyte selected from the group consisting of 3-5-Diiodo-L-Thyronine (LT2), 3-3′-5′-L-Thyroxine (LT3) and L-Thyroxine (LT4). Prior to analyzing the analytes, the method can include one or more of: washing the solid phase extraction column with a methanol solution after the step of binding; eluting the plurality of analytes from the adsorbent with at least one of (i) a mixture of isopropanol and methanol, and (ii) a mixture of ethyl acetate and methanol, to form an eluate; evaporating the eluate; and reconstituting the evaporated eluate with a mixture of methanol and water.