RECOMBINANT PROTEINS EXPRESSION AND PURIFICATION SYSTEM, MARKED WITH SMBP AS FUSION PROTEIN.

摘要

The present disclosure is related to the recombinant proteins area, specifically to the invention of more efficient mechanisms for the proteins of interest production and purification. The present invention determines the use of the SmbP originated from the Nitrosomonas europaea as a fusion protein, allowing the proteins of interest expression in the cytoplasm to then export it to the periplasm; also a recombinant protein purification process is established through affinity chromatography using a functionalized resin with metal ions (Cu+2 or Ni+2). Classic cloning methods are used for the plasmids construction with the periplasmic and cytoplasmic SmbP of Nitrosomonas europaea adding several genes of the proteins of interest (GFP, RFP, NDPK2, LovR, SHY2) for further expression and purification by affinity chromatography using a functionalized resin with metal ions (Cu+2 or Ni+2).