PREPARATION METHOD OF 4D CHITOSAN-BASED THERMOSENSITIVE HYDROGEL

摘要

#$%^&*AU2020100879A420200709.pdf#####ABSTRACT The present invention belongs to the technical field of medical material production and preparation, and relates to a preparation method of a 4D chitosan-based thermosensitive hydrogel. First, chitosan is dissolved in acetic acid solution; a chitosan-based thermosensitive hydrogel is printed by a 4D bioprinter and lyophilized after solvent extraction, to obtain lyophilized chitosan; subsequently, aqueous p-sodium glycerophosphate solution is prepared with ultrapure water and p-sodium glycerophosphate, and then aqueous carboxymethyl chitosan solution is prepared with ultrapure water and aqueous p-sodium glycerophosphate solution are charged into and mixed well with aqueous carboxymethyl chitosan solution to prepare a mixture; finally, the lyophilized chitosan is crosslinked with the mixture to obtain the 4D chitosan-based thermosensitive hydrogel. With scientific and reliable principles thereof, the present invention solves a problem that conventional thermosensitive hydrogels have uneven pore sizes, and improves the entrapment efficiency and ability of limbal stem cells; the present invention has important theoretical significance and broad application prospects in the field of corneal wound healing, providing help for patients who require repair of corneal alkali bums in clinical medicine.