PHASE CONTRAST AND DIFFERENTIAL INTERFERENCE CONTRAST MICRO-IMAGING METHOD BASED ON LIGHT INTENSITY TRANSMISSION EQUATION

摘要

A phase contrast and differential interference contrast micro-imaging method based on a light intensity transmission equation, the method comprising: firstly, collecting three intensity images along an optical axis; secondly, solving a light intensity transmission equation by using deconvolution so as to obtain a quantitative phase diagram; then obtaining, according to a differential interference contrast imaging principle, a light intensity diagram under an imaging mode; and finally, solving, according to a phase contrast imaging principle, a corresponding phase transfer function so as to obtain a light intensity diagram in the imaging mode. The method is capable of endowing a bright field microscope with a capability of realizing phase contrast and differential interference contrast imaging under the premise of not carrying out complicated transformation on a traditional bright field microscope, namely, the same imaging effect as a phase contrast microscope and a differential interference contrast microscope, which are high in cost, complicated in structure and strict in environment condition requirement, can be realized with the advantages of being quantitative, high in speed, low in cost, simple in structure and with less external interference by means of a phase contrast and differential interference contrast algorithm by using a common traditional bright field microscope without adding any complicated device.