PRIMERS FOR HEPATITIS C VIRUS NUCLEIC ACID DETECTION, PROBE, KIT, AND DETECTION METHOD

摘要

Primers for hepatitis C virus nucleic acid detection, a probe, a kit, and a detection method. The primers for hepatitis C virus nucleic acid detection comprise a first primer and a second primer, of which the sequences are respectively as shown by SEQ ID NO.1 and SEQ ID NO.2. The sequence of the probe is as shown by SEQ ID NO.3. The kit comprises said primers and the probe. The hepatitis C virus nucleic acid detection method comprises: mixing a nucleic acid sample to be detected with a PCR liquid by using a sample treatment device, and coating the formed mixed liquid with a droplet generation liquid to form a droplet containing a single nucleic acid or containing no nucleic acid; and performing PCR amplification on the droplet by using the kit, and detecting a PCR amplification product. The kit is low in detection lower limit, high in sensitivity, and good in specificity. The detection method is simple and quick, high in detection efficiency, and low in cost.