Provided is a blood relationship analysis method using genotyping, which comprises: step (A) of analyzing, based on a marker, a genotype of entities whose likelihood is to be checked; step (B) of inputting a genotyping result data as a result of the step (A); step (C) of inputting an allele frequency from the genotyping result data of the step (B); step (D) of comparing whether an allelotype is matched between the entities whose likelihood is to be checked, setting an identity by descent (IBD) formula in accordance with the number of cases with which the allelotype is matched, and applying an IBD constant in accordance with the number of cases of the likelihood to the set IBD formula, and thus extracting a likelihood ratio; and step (E) of confirming a probability result of the highest value of the step (D) to determine the likelihood. According to the present invention, an analysis process is simplified such that analysis can be performed in a short time, thereby increasing correctness, efficiency, simplicity and specialty in business, and providing a correct result in family line confirmation, selection breeding and discharge effect examination (sibling analysis for a discharged seed).
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