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sRNA Multiplex Gene Expression Inhibition System based on Synthetic Regulatory Small RNA and Method of Preparing the Same
sRNA Multiplex Gene Expression Inhibition System based on Synthetic Regulatory Small RNA and Method of Preparing the Same
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机译:基于合成调控小RNA的sRNA多重基因表达抑制系统及其制备方法
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摘要
The present invention relates to a multiplex target gene expression inhibition system based on a synthetic regulatory sRNA and a method of preparing the same, and more specifically, to a combination of vectors comprising a variety of antibiotic-resistance genes, an origin of replication, and a synthetic regulatory sRNA; a method of preparing the same; an sRNA library consisting of the combination of vectors; and a method of screening for a bacterial strain producing a useful substance by using the same. The multiplex target gene expression inhibition system according to the present invention is a gene expression regulation system based on a synthetic regulatory sRNA that, unlike existing gene knock-out methods, can effectively inhibit the expression of a specific gene without altering the sequence of a target gene; comprises various vectors, and thus can be used compatibly with other plasmids usable in prokaryotes; and is designed to enable multiplex introduction, and thus is advantageous in a fast and efficient production of recombinant microorganisms. Furthermore, the present invention provides a technology that enables simple and fast transferring of a genome-level synthetic regulatory sRNA library to a new platform, and the technology is useful in selection of recombinant microorganisms producing useful substances in high capacity. Accordingly, the present invention is useful in the production of recombinant bacterial strains capable of dramatically increasing the production efficiency of a variety of medically useful metabolites.
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