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An inductive plasmid display system for selecting a plasmid encoding a protein with specific properties expressed

机译:感应质粒展示系统,用于选择编码表达特定特性的蛋白质的质粒

摘要

The present invention relates to an induction plasmid display system for selecting a plasmid encoding a protein expressing a specific property. In other words, unlike the existing system, the present inventors can display a protein plasmid having desired properties in a large gene mutant library generated through molecular evolution in vitro by forming a fusion protein with a plasmid, thereby displaying an induced plasmid that can be efficiently selected in a short time. Since the system was developed, it is possible to obtain a high-value product more economically and efficiently by selecting a protein having high activity or maintaining activity through the present invention and then applying it to microorganisms for production of substances of high industrial value. It is expected.
机译:诱导质粒展示系统技术领域本发明涉及用于选择编码表达特定特性的蛋白质的质粒的诱导质粒展示系统。换句话说,与现有系统不同,本发明人可以通过与质粒形成融合蛋白,在通过体外分子进化产生的大基因突变体文库中显示具有所需特性的蛋白质质粒,从而展示可以有效地诱导的质粒。在短时间内选择。由于开发了该系统,通过本发明选择具有高活性或保持活性的蛋白质,然后将其应用于微生物以生产具有高工业价值的物质,可以更经济和有效地获得高价值的产品。可以预料的。

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