Cas10/Csm4 The preparation method of cyclic oligoadenylate using Cas10/Csm4

摘要

The present invention relates to a method for preparing cyclic oligoadenylate (cOA) using Cas10/Csm4, and specifically, a CRISPR III-A type Cas10/Csm4 subcomplex, a target RNA upon reaction of the Cas10/Csm4 subcomplex and ATP A method for preparing a novel material without cyclic oligo-adenylate (cOA), cOA prepared by the above manufacturing method, a composition for cOA synthesis including the Cas10/Csm4 subcomplex, and cOA It relates to a kit for synthesis.