SIGNAL PEPTIDE FUSION PARTNERS FACILITATING LISTERIAL EXPRESSION OF ANTIGENIC SEQUENCES AND METHODS OF PREPARATION AND USE THEREOF

摘要

The present invention provides efficient expression and secretion of an antigen of interest into the cytosol of a host cell, and as a fusion partner of the N-terminus in the translation reading frame with the selected recombinant encoded protein antigen, Listeria or other bacterial signal peptide/secretion Functionally linking the cheprons provides nucleic acids, expression systems and vaccine strains that elicit effective CD4 and CD8 T cell responses. These N-terminal fusion partners are deleted (by actual deletion, by mutation, or by a combination of these approaches) for the sequence and/or for any PEST sequence that is unique for a particular hydrophobic residue.