The method for determining antigenic differences of viruses of the genus Arenavirus family Arenaviridae

摘要

FIELD: microbiology; virology.SUBSTANCE: disclosed is a method of identifying Arenaviridae family Arenavirus viruses by negative colonies damping reaction involving preparing a three-day monolayer of Vero B cells in plastic bottles; preparing dilutions of analyzed and control samples of arenaviruses; introducing into each of flasks 0.5 ml of the respective dilution, removal from the inoculant flasks after 60 min of incubation; primary agar coating preparation, containing by 0.1 % by volume of antibody-containing substrate, and its introduction into 8 ml into each bottle; preparing 0.1 % neutral red solution and staining cell monolayer 96 hours after applying the primary agar coating; taking into account the amount and determining the size of negative colonies formed by analyzed arenaviruses 24 hours after application of 0.1 % neutral red; calculating indices of the relative size of negative colonies and identifying the virus by reducing the size of negative colonies by at least 2-fold when the homologous virus-antibody pair interacts.EFFECT: invention provides the possibility of identifying the analyzed arenavirus by adding 0_1 % (by volume) of the antibody-containing substrate to the primary agar coating, as well as reducing the analysis duration.1 cl, 6 tbl, 2 ex