RECOMBINANT PLASMID DNA AAV SYNH1-2_SHRNA FREUD-1, PROVIDING SYNTHESIS OF SHPHK, SUPPRESSING EXPRESSION OF GENE CODING SELECTIVE SILENSER 5-HT1A OF RECEPTOR FREUD-1, IN BRAINS OF MAMMALS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, specifically to recombinant expression plasmids, and can be used to synthesize the small hairpin RNA (shRNA) inhibiting the expression of the gene coding selective silenser 5-HT1A receptor Freud-1 in the brain of mammals. AAV SynH1-2_shRNA Freud-1 plasmid is designed which is packaged into an adeno-associated viral capsid to ensure delivery of a construct into mammalian neurons.;EFFECT: transcription of shRNA coded by plasmid AAV SynH1-2_shRNA Freud-1 in mouse neurons reduces expression of selective silenser of serotonin 5-HT1A receptor Freud-1, thus leading to amplification of 5-HT1A receptor expression in brains of mice and, as a result, having antidepressant action.;1 cl, 5 ex, 8 dwg