PROBLEM TO BE SOLVED: To provide a novel pipecolic acid 4-hydroxylase and a novel method for inexpensively and conveniently producing 4-hydroxyamino acid, particularly optically active 4-hydroxyamino acid. SOLUTION: Pipecolic acid 4-position hydroxylase protein having an activity of acting on L-pipecolic acid to produce trans-4-hydroxy-L-pipecolic acid in the presence of 2-oxoglutarate and divalent iron ion, And/or a cell containing the same, a preparation of the same or a culture solution obtained by culturing the same to act on α-amino acid to produce 4-hydroxyamino acid, Amino acid production method. [Selection diagram] None
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