首页> 中国专利> 检测灰霉病菌群体对甲氧基丙烯酸酯类杀菌剂抗性频率的引物序列及方法

检测灰霉病菌群体对甲氧基丙烯酸酯类杀菌剂抗性频率的引物序列及方法

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摘要

本发明公开了2组引物，还公开了利用2组引物检测灰霉病菌群体对甲氧基丙烯酸酯类杀菌剂抗性频率的方法，包括以下步骤：提取待测灰霉病菌群体的DNA；以该DNA为模板，利用第一组引物和第二组引物分别进行实时定量PCR扩增并记录其达到荧光阈值的循环数；参照实时定量PCR测定待测灰霉病菌群体的DNA总量的标准曲线以及实时定量PCR测定抗甲氧基丙烯酸酯类杀菌剂的灰霉病菌的DNA量的标准曲线，得到待测灰霉病菌群体的DNA总量Q以及抗甲氧基丙烯酸酯类杀菌剂的灰霉病菌的DNA量Q

著录项

公开/公告号CN101760554B

专利类型发明专利
公开/公告日2012-05-23

原文格式PDF
申请/专利权人浙江大学;
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申请/专利号CN200910155628.8
发明设计人蒋金花;吴剑丙;马忠华;
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申请日2009-12-18
分类号
代理机构杭州天勤知识产权代理有限公司;
代理人胡红娟
地址 310027 浙江省杭州市西湖区浙大路38号
入库时间 2022-08-23 09:09:40

法律信息

法律状态公告日

法律状态信息

法律状态
2015-02-04

未缴年费专利权终止 IPC(主分类):C12Q 1/68 授权公告日:20120523 终止日期:20131218 申请日:20091218

专利权的终止
2012-05-23

授权

授权
2010-08-25

实质审查的生效 IPC(主分类):C12Q 1/68 申请日:20091218

实质审查的生效
2010-06-30

公开

公开

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1. 检测灰霉病菌群体对甲氧基丙烯酸酯类杀菌剂抗性频率的引物序列及方法 [P] . 中国专利： CN101760554B . 2012.05.23
2. 用于检测指状青霉菌对抑霉唑抗性频率的引物序列及其检测方法 [P] . 中国专利： CN101270384B . 2010.12.01
3. Processes for detecting a mutation in the nucleic acid to detect fungal and fungal resistance to an analogue of estobilurina,Fungal DNA sequence encoding all or part of a proteu00ecna of cytochrome b, cytochrome b proteu00ecna fungal.The allele specific oligonucleotu00ecdeo capable of connecting to a sequence of fungal nucleic acid, initiator of diagnosis or diagnostic oligonucleotu00ecdeo capable of connecting to a template.One or more diagnostic primers, probe the allele specific oligonucleotu00ecdeo, diagnostic kit to detect fungal pathogen resistance, process plant, a fungicide.To detect and quantify the frequency of mutation, to select a fungicide active and their nu00ecveis optimal implementation, and to control the fungal infection of a collection, and,A computer legu00ecvel [P] . BR0010163A . 2002-01-15

机译：用于检测核酸突变的方法，以检测对食管胆囊炎类似物的真菌和真菌抗性，真菌DNA序列编码全部或部分细胞色素b蛋白，细胞色素b蛋白真菌，等位基因特异性寡核苷酸能够连接到真菌核酸序列的诊断或诊断寡核苷酸的启动子，能够连接到模板。一个或多个诊断引物，探查等位基因特异性寡核苷酸，诊断试剂盒以检测真菌病原体抗性，加工厂要检测和量化突变的频率，选择一种活性杀菌剂及其最佳实施方案，控制一个集合体的真菌感染以及计算机支路，
4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

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