首页> 中国专利> 革兰氏阳性菌表达系统在表达腐败梭菌毒素中的应用、腐败梭菌α毒素的制备方法和疫苗

革兰氏阳性菌表达系统在表达腐败梭菌毒素中的应用、腐败梭菌α毒素的制备方法和疫苗

页面导航

摘要
著录项
相似文献

摘要

本发明提供了一种革兰氏阳性菌表达系统在表达腐败梭菌毒素中的应用、腐败梭菌α毒素的制备方法和疫苗，涉及生物技术领域。采用革兰氏阳性菌表达系统表达腐败梭菌毒素实现了腐败梭菌毒素的胞外分泌表达，离心收集上清，就可以获得表达量高且纯度较好的目的蛋白，降低目的蛋白纯化和内毒素去除工艺生产成本。

著录项

公开/公告号CN111944838B

专利类型发明专利
公开/公告日2022.08.16

原文格式PDF
申请/专利权人天康制药（苏州）有限公司;
展开▼

申请/专利号CN202010907945.7
发明设计人贺笋;王钢;张飞;何海;丁占强;周婷婷;韩瑞凯;杜晓杰;李延涛;
展开▼

申请日2020.09.02
分类号C12N15/77(2006.01);C12N15/75(2006.01);C12N15/74(2006.01);C07K14/33(2006.01);C12N1/21(2006.01);G01N33/569(2006.01);G01N33/577(2006.01);A61K39/08(2006.01);A61P31/04(2006.01);C12R1/45(2006.01);C12R1/125(2006.01);C12R1/01(2006.01);
代理机构
代理人
地址 215000 江苏省苏州市中国（江苏）自由贸易试验区苏州片区苏州工业园区现代大道88号6层6-013工位（集群登记）
入库时间 2022-09-26 23:16:11

相似文献

专利
中文文献
外文文献

1. 革兰氏阳性菌表达系统在表达腐败梭菌毒素中的应用、腐败梭菌α毒素的制备方法和疫苗 [P] . 中国专利： CN111944838A . 2020-11-17
2. 腐败梭菌α毒素突变体、表达其的基因、制备方法和腐败梭菌疫苗 [P] . 中国专利： CN109111509B . 2021-08-13
3. Detoxified immunogenic derivative of clostridium perfringens b-toxin or an immunogenic fragment thereof, nucleotide sequence comprising a mutant DNA fragment, clostriduim perfringensm expression system based on a gram-positive bacterium, vaccine to combat clostridium perfrigens infection, process for the preparation of native b-toxin from clostridium perfrigens, and, process for preparing a vaccine to combat infection of clostridium perfrigens. [P] . BR9802361A . 2000-01-11

机译：产气荚膜梭菌b毒素的解毒免疫原性衍生物或其免疫原性片段，包含突变DNA片段的核苷酸序列，基于革兰氏阳性细菌的产气荚膜梭菌表达系统，抗付产气荚膜梭菌感染的疫苗，制备天然b-产高产梭状芽胞杆菌的毒素，以及制备疫苗来抵抗产高产梭状芽胞杆菌感染的方法。
4. Fusion protein Fusion protein The soluble composition of the host cell material Process to generate a neutralizing anti-toxin directed against Clostridium botulinum toxin type A antibody to purify a recombinant fusion protein derived from a Clostridium botulinum toxin type A process to generate an antitoxin neutralizing directed against clostridium difficile toxin type b antibody treatment process process to generate a neutralizing antitoxin directed against clostridium difficile type a toxin composition process to vaccinate an individual to produce neutralizing antitoxin directed against clostridium difficile toxin processing the detection of clostridium antigens difficile in an ample process of purification of toxins from clostridium difficile from a culture and process of generation of a solid dosage form of a bird antitoxin directed against a protein of clostridial toxin [P] . BR9509903A . 1997-11-25

机译：融合蛋白融合蛋白宿主细胞材料的可溶性组成物产生针对A型肉毒梭菌毒素的中和抗毒素的过程纯化源自A型肉毒杆菌毒素的重组融合蛋白的抗体产生针对人肉毒杆菌的抗毒素的过程B型艰难梭菌毒素抗体处理工艺过程，产生针对A艰难梭菌毒素的中和抗毒素组合物，对个体进行疫苗接种，以生产针对艰难梭菌毒素的中和抗毒素，在充足的纯化纯化过程中检测艰难梭菌抗原来源于梭状芽胞杆菌毒素蛋白的鸟抗毒素固体剂型的培养物和固体剂型产生过程中的艰难梭菌毒素
5. A DNA fragment which encodes a Pasteurella multocida toxin, or an immunogenic subsequence or analogue thereof, an expression vector which comprises the DNA fragment, a process for preparing a Pasteurella multocida toxin, and the use of a Pasteurella multocida toxin sequence or toxin sequence analogue for producing a vaccine [P] . DK169749B1 . 1995-02-13

机译：编码多杀巴斯德氏菌毒素或其免疫原性亚序列或类似物的DNA片段，包含该DNA片段的表达载体，制备多杀巴斯德氏菌毒素的方法以及多杀巴斯德氏菌毒素序列或毒素序列类似物的用途生产疫苗