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用于鲟鱼性别鉴定的特异性DNA片段SSM2及应用

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摘要

本发明属于水产养殖领域中的鱼类性别鉴定领域，具体用于鲟鱼性别鉴定的特异性DNA片段SSM2及应用，本发明是通过高通量测序和比较基因组方法获得了鲟鱼雌性个体特异性DNA片段，针对该片段设计引物，能够对9中鲟鱼进行准确快速的性别鉴定，本发明的鉴定方法操作简单、快捷、准确、且对鲟鱼伤害极小等特点，解决了鲟鱼性别鉴定的难题，将有助于鲟鱼养殖产业的快速发展。

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公开/公告号CN111471775B

专利类型发明专利
公开/公告日2021-04-30

原文格式PDF
申请/专利权人中国水产科学研究院长江水产研究所;
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申请/专利号CN201910066586.4
发明设计人阮瑞;李创举;危起伟;岳华梅;叶欢;杜浩;刘志刚;冯彤;阮珏;
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申请日2019-01-24
分类号C12Q1/6888(20180101);C12Q1/6879(20180101);C12N15/11(20060101);
代理机构42001 武汉宇晨专利事务所;
代理人龚莹莹
地址 430223 湖北省武汉市东湖新技术开发区武大园一路8号
入库时间 2022-08-23 11:42:41

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1. 用于鲟鱼性别鉴定的特异性DNA片段SSM2及应用 [P] . 中国专利： CN111471775B . 2021.04.30
2. 用于鲟鱼性别鉴定的特异性DNA片段SSM1及应用 [P] . 中国专利： CN111471756B . 2021.04.13
3. Nucleotide sequences of primers specific for the terminal areas of the tested DNA fragment conjugated with Rfo restorer gene for CMS ogura in rape, method for DNA amplification by polymerase chain reaction (PCR) with primers specific for genomic DNA fragment coupled with Rfo gene for CMS ogura in rape, process for the preparation of the nucleotide sequence of the DNA fragment conjugated the Rfo restorer gene for CMS ogura in rape, process for the preparation of SCAR type DNA marker for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility, method for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility and test kit for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility [P] . PL212433B1 . 2012-10-31

机译：油菜中与CMS ogura的Rfo恢复基因偶联的被测DNA片段末端区域特异的引物的核苷酸序列，通过基因组DNA片段的特异性引物与CMS ogura的Rfo基因偶联的聚合酶链反应（PCR）扩增DNA的方法在油菜中，制备与油菜CMS ogura的Rfo恢复子基因偶联的DNA片段的核苷酸序列的方法，在SCAR型DNA标记物的制备方法中用于鉴定含ogura基因的Rfo恢复子基因的油菜植物雄性不育，带有Rfo恢复基因的油菜植株的ogura基因胞质雄性不育的鉴定方法和试剂盒
4. DNA amplification primers and species-specific, gender specific and universal probes for detection and identif. fast bacterial pathogens and fungal and genes common resist. antibiotic-associated clinical specimens for diagnosis in microbiology lab. [P] . ES2329202T3 . 2009-11-23

机译： DNA扩增引物和物种特异性，性别特异性和通用探针，用于检测和鉴定。快速细菌病原体与真菌和基因共同抵抗。抗生素相关的临床标本，可在微生物实验室进行诊断。
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法