一种靶向测序试剂盒及其使用方法及靶向测序方法

摘要

本发明涉及基因突变检测领域，具体讲，涉及一种靶向测序试剂盒及其使用方法及靶向测序方法。本发明的靶向测序试剂盒包括目的片段扩增试剂，目的片段扩增试剂包括用于扩增人B细胞慢性淋巴细胞增殖性疾病的突变位点的特异性引物。本发明试剂盒及方法利用靶向测序对B细胞慢性淋巴细胞增殖性疾病相关基因的多个目标区域同时进行深度测序，提高了检测效率，同时还能提高检测的准确性和灵敏度，并能进一步对大量样品同时进行多区域测序。

说明书

技术领域

本发明涉及基因突变检测领域，具体讲，涉及一种靶向测序试剂盒及其使用方法及靶向测序方法。

背景技术

B细胞慢性淋巴细胞增殖性疾病(B-CLPDs)是包括慢性淋巴细胞白血病(CLL)、毛细胞白血病(HCL)、毛细胞白血病变异型(HCL-V)等原发白血病，以及白血病期的套细胞淋巴瘤(MCL)、滤泡细胞淋巴瘤及各类边缘区的淋巴瘤在内的一大类疾病。

B细胞慢性淋巴细胞增殖性疾病(B-CLPDs)的精确诊断十分重要，不同亚型的B-CLPD临床预后和治疗策略差别较大，对于无治疗指征的CLL，可以采取随访观察方式，而对于绝大部分的MCL，则适合选择强度较大的化疗。大部分的B-CLPD在形态学上均表现为成熟的小淋巴细胞，单纯地依靠形态学很难准确诊断，尽管免疫分型联合细胞遗传学大大的提高了B-CLPD的诊断准确性，但是仍有一部分B-CLPD，包括淋巴浆细胞淋巴瘤/巨球蛋白血症(LPL/WM)，边缘区淋巴瘤(MZL)的诊断仍然十分困难。对于同一类B-CLPD，其临床预后差异也比较悬殊。近年来，B-CLPDs相关的分子突变的发现，有望优化B-CLPD的精确诊断，并提高B-CLPD的预后分层。

淋巴浆细胞淋巴瘤/巨球蛋白血症(LPL/WM)与其他B-CLPD的鉴别具有一定的难度，尤其在其他的B-CLPD形态学上伴有浆细胞分化的情况下。研究发现，90％以上的LPL/WM具有MYD88 L265P突变，而对于多发性骨髓瘤和边缘区淋巴瘤，则不会或极少出现MYD88L265P的突变。

尽管毛细胞白血病(HCL)的具有特征性的形态学特征，但是由于毛细胞白血病变异型(HCL-V)、脾脏边缘区淋巴瘤(SMZL)、脾脏弥漫红髓小B细胞淋巴瘤均可以表现出毛细胞的形态，所以，不能单纯的形态学进行HCL的诊断。研究发现，几乎所有的HCL均具有BRAFV600E突变。

脾脏边缘区淋巴瘤(SMZL)主要表达为脾脏肿大，部分病例会出现骨髓和/或外周血的累及。单纯依赖形态学与免疫表型将很难将SMZL与其B细胞淋巴瘤鉴别出来。约有25％的SMZL具有NOTCH2基因的突变，而在非脾脏的边缘区淋巴瘤中，比例较低仅为5％；在其他的小B细胞淋巴瘤中则不会出现NOTCH2突变。这一结果提示NOTCH2突变的检测可以用于SMZL的诊断与鉴别诊断。研究发现，在脾脏边缘区淋巴瘤内，KLF2的突变约占到40％，而在其他的B细胞淋巴瘤中则很少出现KLF2突变。这一研究结果表明，KLF2的检测可以用于SMZL的鉴别。综上，NOTCH2和KLF2的检测可以用于SMZL的辅助诊断。

最近的研究表明，另外一种类型的边缘区淋巴瘤-淋巴结边缘区淋巴瘤具有PTPRD的突变，而这一突变在其他的B细胞淋巴瘤，包括其他类型的边缘区淋巴瘤中都不会出现，所以提示我们，这个标志，很有可能可以用于边缘区淋巴瘤的诊断。

在慢性淋巴细胞白血病(CLL)当中，不同患者的预后具有显著的差异。多项研究表明，CLL中的分子突变可以用于预测患者的预后。多个研究表明，具有TP53、SF3B1、NOCTH1以及BIRC3突变的患者预后较差。对于具有TP53亚克隆突变的患者，其预后有具有TP53主克隆突变的患者一样较差。此外，来自CLL8临床试验的数据表明，NOTCH1突变的患者使用FCR方案相对于FC方案来讲，并没有使患者获益，提示NOTCH1突变的检测在指导治疗方案的选择上亦具有一定的意义。此外，对于复发难治的患者，NOTCH1突变的患者使用CD52单抗获益更多。这些研究结果均表明，CLL的分子突变可以用于CLL患者的预后评估。

对于其他B-CLPD，分子突变亦具有预后价值。这些新的分子标志的发现，为分子突变用于B-CLPD的诊断奠定了基础。在SMZL中，NOTCH2突变的患者，其预后要显著差于NOTCH2无突变的患者。在MCL中，TP53突变的患者也显著较差。在滤泡淋巴瘤中，EZH2、ARID1A、MEF2B、EP300、FOXO1、CREBBP、和CARD11突变的纳入，可以进一步优化预后分层。

靶向测序，尤其是深度靶向测序，可以同时对数十个甚至数百个基因进行检测，除此之外，深度靶向测序可以检测到Sanger测序检测不到的突变，敏感性较高。

发明内容

鉴于此，本发明提供一种靶向测序试剂盒及其使用方法及靶向测序方法。

本发明第一方面涉及一种靶向测序试剂盒，所述靶向测序试剂盒包括目的片段扩增试剂，所述目的片段扩增试剂包括用于扩增人B细胞慢性淋巴细胞增殖性疾病的突变位点的特异性引物；

所述特异性引物的设计区域为KMT2D CDS区域、BCL2 CDS区域、CREBBP CDS区域、EZH2 CDS区域、CARD11 CDS区域、EP300 CDS区域、TNFRSF14 CDS区域、RRAGC CDS区域、ATP6V1B2 CDS区域、ATP6AP1 CDS区域、PTPRD CDS区域、KLF2 CDS区域、NOTCH2 CDS区域、MYD88 CDS区域、BIRC3 CDS区域、ARID1A CDS区域、CXCR4 CDS区域、CDKN1B CDS区域、MAP2K1 CDS区域、SF3B1 CDS区域、ATM CDS区域、POT1 CDS区域、CHD2 CDS区域、XPO1 CDS区域、RPS15 CDS区域、IGLL5 CDS区域、EGR2 CDS区域、FBXW7 CDS区域、SAMHD1 CDS区域、LRP1B CDS区域、CCND1 CDS区域、NFKBIE CDS区域、TET2 CDS区域、PLCG2 CDS区域、BTK CDS区域、FOXO1 CDS区域、FAT1 CDS区域、TP53 Exon区域、NOTCH1 Exon区域、BRAF V600E突变位点、KRAS G12突变位点、KRAS G13突变位点、KRAS Q61突变位点、KRAS A146突变位点。

可选的，所述用于扩增人B细胞慢性淋巴细胞增殖性疾病的突变位点的特异性引物分别为：SEQ ID NO:1～SEQ ID NO:2120。

可选的，所述试剂盒还包括目的片段纯化试剂，用于对目的片段扩增后的产物进行纯化。

可选的，所述目的片段纯化试剂包括：XP Kit。

可选的，所述试剂盒还包括目的片段纯化产物定量试剂，用于对纯化后的目的片段进行定量。

可选的，所述试剂盒还包括文库建库试剂，具体包括PCR产物末端修补和末端加polyA反应液、连接barcode接头反应液、barcode产物纯化试剂、文库富集试剂和文库纯化试剂。

可选的，所述PCR产物末端修补和末端加polyA反应液包括：KAPA End Repair&A-Tailing Buffer和KAPA End Repair&A-Tailing Enzyme；

可选的，所述连接barcode接头反应液包括：Ligation Buffer、DNA连接酶和PKR YType Adapter Kit Index X；

可选的，所述barcode产物的纯化试剂包括：XP Beads；

可选的，所述文库富集试剂包括：2X KAPA HiFi HotStart ReadyMix和10XLibrary Amplification Primer Mix；

所述文库纯化试剂包括：XP Beads。

其中，KAPA End Repair&A-Tailing Buffer、KAPA End Repair&A-TailingEnzyme、Ligation Buffer、DNA连接酶、PKR Y Type Adapter Kit Index X、XP Beads、2X KAPA HiFi HotStart ReadyMix，10X Library AmplificationPrimer Mix和XP Beads均为市售产品。

可选的，所述试剂盒还包括样本处理试剂，用于获得待测样本的DNA。

可选的，所述待测样本包括全血、血清、血浆和组织样品；所述组织样品包括石蜡包埋组织、新鲜组织和冰冻切片。

可选的，所述试剂盒还包括样本PCR扩增试剂，用于扩增待测样本的DNA。

本发明还涉及上述靶向测序试剂盒的使用方法，至少包括以下步骤：

(1)样本处理：用于获得待测样本的DNA；

(2)PCR扩增：扩增待测样本的DNA；

(3)目的片段扩增：用于扩增人B细胞慢性淋巴细胞增殖性疾病的突变位点；

(4)目的片段纯化：用于对目的片段扩增后的产物进行纯化；

(5)目的片段纯化产物定量：用于对纯化后的目的片段进行定量；

(6)文库建库；

包括PCR产物末端修补和末端加polyA、连接barcode接头、barcode产物纯化、文库富集、文库纯化的步骤；

(7)测序。

本发明还涉及一种靶向测序方法，至少包括以下步骤：

(1)样本处理：用于获得待测样本的DNA；

(2)PCR扩增：扩增待测样本的DNA；

(3)目的片段扩增：用于扩增人B细胞慢性淋巴细胞增殖性疾病的突变位点；

所述突变位点的设计区域为KMT2D CDS区域、BCL2 CDS区域、CREBBP CDS区域、EZH2 CDS区域、CARD11 CDS区域、EP300 CDS区域、TNFRSF14 CDS区域、RRAGC CDS区域、ATP6V1B2 CDS区域、ATP6AP1 CDS区域、PTPRD CDS区域、KLF2 CDS区域、NOTCH2 CDS区域、MYD88 CDS区域、BIRC3 CDS区域、ARID1A CDS区域、CXCR4 CDS区域、CDKN1B CDS区域、MAP2K1 CDS区域、SF3B1 CDS区域、ATM CDS区域、POT1 CDS区域、CHD2 CDS区域、XPO1 CDS区域、RPS15 CDS区域、IGLL5 CDS区域、EGR2 CDS区域、FBXW7CDS区域、SAMHD1CDS区域、LRP1BCDS区域、CCND1 CDS区域、NFKBIE CDS区域、TET2 CDS区域、PLCG2 CDS区域、BTK CDS区域、FOXO1 CDS区域、FAT1 CDS区域、TP53 Exon区域、NOTCH1 Exon区域、BRAF V600E突变位点、KRAS G12突变位点、KRAS G13突变位点、KRAS Q61突变位点、KRAS A146突变位点；

用于扩增所述突变位点的特异性引物分别为：SEQ ID NO:1～SEQ ID NO:2120；

(4)目的片段纯化：用于对目的片段扩增后的产物进行纯化；

(5)目的片段纯化产物定量：用于对纯化后的目的片段进行定量；

(6)文库建库；

包括PCR产物末端修补和末端加polyA、连接barcode接头、barcode产物纯化、文库富集、文库纯化的步骤；

(7)测序。

本发明至少具有以下有益的效果：

本发明试剂盒及方法利用靶向测序对B细胞慢性淋巴细胞增殖性疾病(B-CLPDs)相关基因的多个目标区域同时进行深度测序，提高了检测效率，同时还能提高检测的准确性和灵敏度，并能进一步对大量样品同时进行多区域测序。

现有技术中，Sanger测序技术每次只能对一个样品的某一段区域进行测序，要实现对多个目标区域的测序，只能是通过多次反应来实现。而在本发明中，测序文库中的各个分子经过单分子扩增后，每个测序文库分子均形成单分子拷贝阵列，各单分子拷贝阵列在进行高通量基因测序时处于不同的位置，使得测序引物与单分子拷贝阵列之间的杂交，以及在酶作用下的延伸反应可同时进行，相互之间互不干扰。因此，可以同时对大量的(成百万上千万，甚至上亿、数十亿的)单分子拷贝阵列同时进行测序反应，然后通过采集相应的信号，进而获得所需的序列信息，且测序的灵敏度较Sanger更高。

附图说明

图1为文库建库过程中步骤ii的加样方式；

图2为文库建库过程中步骤v的加样方式；

图3为文库建库过程中步骤vi的加样方式；

图4为文库建库过程中步骤vii的加样方式；

图5为文库建库过程中步骤viii的加样方式；

图6为实施例3中样本3632数字PCR结果；

图7为实施例3中样本3451数字PCR的结果；

图8为实施例3中样本3460数字PCR的结果。

下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本申请而不用于限制本发明的范围。

具体实施方式

下面通过实施例和对比例进一步说明本发明，这些实施例只是用于说明本发明，本发明不限于以下实施例。凡是对本发明技术方案进行修改或者等同替换，而不脱离本发明技术方案的精神和范围，均应涵盖在本发明的保护范围中。

本发明实施例涉及一种靶向测序试剂盒，包括目的片段扩增试剂，目的片段扩增试剂包括用于扩增人B细胞慢性淋巴细胞增殖性疾病的突变位点的特异性引物；特异性引物的设计区域为KMT2D CDS区域、BCL2 CDS区域、CREBBP CDS区域、EZH2 CDS区域、CARD11CDS区域、EP300 CDS区域、TNFRSF14 CDS区域、RRAGC CDS区域、ATP6V1B2 CDS区域、ATP6AP1CDS区域、PTPRD CDS区域、KLF2 CDS区域、NOTCH2 CDS区域、MYD88 CDS区域、BIRC3 CDS区域、ARID1A CDS区域、CXCR4 CDS区域、CDKN1B CDS区域、MAP2K1 CDS区域、SF3B1 CDS区域、ATM CDS区域、POT1 CDS区域、CHD2 CDS区域、XPO1 CDS区域、RPS15 CDS区域、IGLL5 CDS区域、EGR2 CDS区域、FBXW7 CDS区域、SAMHD1 CDS区域、LRP1B CDS区域、CCND1 CDS区域、NFKBIE CDS区域、TET2 CDS区域、PLCG2 CDS区域、BTK CDS区域、FOXO1 CDS区域、FAT1 CDS区域、TP53 Exon区域、NOTCH1 Exon区域、BRAF V600E突变位点、KRAS G12突变位点、KRASG13突变位点、KRAS Q61突变位点、KRAS A146突变位点。本发明利用靶向测序技术同时对多个区域和突变位点进行深度测序，大幅度提高了检测效率，同时还能提高检测的准确性和灵敏度，并能进一步对大量样品同时进行多区域测序。

具体的，用于扩增人B细胞慢性淋巴细胞增殖性疾病的突变位点的特异性引物分别为：SEQ ID NO:1～SEQ ID NO:2120。

SEQ ID NO:1～SEQ ID NO:2120的具体序列为：

SEQ ID NO:1 CCTTGGCCTCACACAGGAAAA

SEQ ID NO:2 CCTGTGATGCTGATGATGCTGATAA

SEQ ID NO:3 GCACTTGGAAGGGCTCCATAA

SEQ ID NO:4 CCCTTCTCCGATGCTTTCTGAA

SEQ ID NO:5 GCAGAGGGTTGTATTGGTTCG

SEQ ID NO:6 CCAAAACAGACCATTTACCAAAAGCT

SEQ ID NO:7 CCGTTCTTGCAGTTGTTTCCTG

SEQ ID NO:8 TCCAAATAATGTGATCCCAAAGCAAGAT

SEQ ID NO:9 CCTCAGAAGATGATCCACTGCCTA

SEQ ID NO:10 AACCACTGCTGCAGAACTTGATA

SEQ ID NO:11 CGTTAACTGCAGATGTCTGCTGA

SEQ ID NO:12 GAGCTGAGTTTGGCTCTTTTGG

SEQ ID NO:13 GGCACTTTTAAGAGTTATTAGTAGGCAGA

SEQ ID NO:14 GTGTGCCTTTTTGTGTGAGTGAA

SEQ ID NO:15 GGAATCTGTATCCTTCATGAATTCTCTCAC

SEQ ID NO:16 CGTTTCGATGCCTTCATCTGCT

SEQ ID NO:17 ATGAGATACCTTCCTCAAAGTTTATGACTG

SEQ ID NO:18 CACTCGTTGCAGGTGTAGACAA

SEQ ID NO:19 CTACCTCCTTGTGCTTCTCCATG

SEQ ID NO:20 CTCCCTAAATAAGCACGTGACTTGT

SEQ ID NO:21 TCCTATTCCTGGGTTGATACTAGAGC

SEQ ID NO:22 GGTTCCATCACTCCATTCCCTT

SEQ ID NO:23 CAATTTCACAAAGGCATTCAGATCTAACA

SEQ ID NO:24 CACAGCTTAAGAACCATGCCACTA

SEQ ID NO:25 TGCATATAAAAACAAAATGACAGCACAGTT

SEQ ID NO:26 GGGACCCAATCTGCTGCTT

SEQ ID NO:27 TCTGTTTTAATGTAGTTTGCTTCTACACCA

SEQ ID NO:28 GCCATTAGCTGCGGGTTTGA

SEQ ID NO:29 CAATATTGCAACATTCTACATGGTCCAA

SEQ ID NO:30 CATTAATAAAGCCCTTTGGGAAGGCTA

SEQ ID NO:31 GCAACAAACATGACAATTTAACAAACTGG

SEQ ID NO:32 TTTGAAACTGTCTTTGTGAACTTGGAAG

SEQ ID NO:33 AGACCATGCCTCAAAAGAGATTTCT

SEQ ID NO:34 AGTGATTGAGCCTGTAGTGATATTTCC

SEQ ID NO:35 ACCAAGTAAAATAACCACAATCTCAACGA

SEQ ID NO:36 AGGGTGCTTCAAATCAGCCTTTA

SEQ ID NO:37 GTAGCCAGACCAGCAGCCTAAA

SEQ ID NO:38 ATTTCACTTTTCTTCTCCTCGTGGAT

SEQ ID NO:39 TCCGACACTGGAAGGGCTTA

SEQ ID NO:40 GGAGAAAGAATAAGTGAAGAGAACAGCT

SEQ ID NO:41 CTGCACAGCCAGAATAAGGAGGA

SEQ ID NO:42 GCATTTTGTGTGAGCCAAGAATACA

SEQ ID NO:43 GCACCCACTTCCAGTCTTCAAA

SEQ ID NO:44 CTTGGAGCACGACTTACCAGAT

SEQ ID NO:45 CTGCTCCAGTCAAGAGACTTCTG

SEQ ID NO:46 GAGAATGATTTTCAGGAACTGAATTAGCC

SEQ ID NO:47 GTTTCCACTTGCAGAGTCTTTTCAC

SEQ ID NO:48 TCCTGTGTAAACCAATTTATATGCCCTT

SEQ ID NO:49 TGCTGACATGATATTACAGTGGTAGGA

SEQ ID NO:50 CGCAACTGGACTTTCTCTCACA

SEQ ID NO:51 TTCACAGCGGCTACCCGAGAAT

SEQ ID NO:52 TCTCCCTTTGGAGGACTTCCA

SEQ ID NO:53 CCCTGGTAGACCAAGTCTGTGA

SEQ ID NO:54 ACTAAACAAAGGACAAGGAGCCATT

SEQ ID NO:55 CCCTCATCCCTTTCAGGGAAGA

SEQ ID NO:56 TTAGAATTCATCTTTGGAACAGAAATGGGA

SEQ ID NO:57 CCCACCCTAATCCTGTGTTTCTT

SEQ ID NO:58 TTTTGAACTTGTCTGGAAAACAATGAGATC

SEQ ID NO:59 GGAGTCAAAGTTAAGACAAGACAGAGT

SEQ ID NO:60 GAGCTGCTGTAAGAACAATAAAGGC

SEQ ID NO:61 AAAAACTGATTGAGGACAGAAGATACACA

SEQ ID NO:62 CGTGGTCCCATTTTACGCATTAC

SEQ ID NO:63 GCTAGGCTAAGCTATGATGTTCCTTAGA

SEQ ID NO:64 TGGAGAAGGAGATTGGAACCTGT

SEQ ID NO:65 ATATATAGAGAGAGAGAGTTCCTCCTGGAA

SEQ ID NO:66 GCAGGGCATGCATCAGATATTC

SEQ ID NO:67 CAGTTGGAGCCCTCGTTACAG

SEQ ID NO:68 AAAAGATAACCTCACACCAGAAATTCCA

SEQ ID NO:69 GGACATGATCATCCTACCCGAGAT

SEQ ID NO:70 GCCTGAAATTGGGCCACTTTTT

SEQ ID NO:71 CGGACCAAAGCGATCTCTTCTG

SEQ ID NO:72 ACACTTTCACTGATAACTGTTAACAGACAA

SEQ ID NO:73 CTCTTATAAAGGCTTCCGGGTCA

SEQ ID NO:74 AAAAAGAGACTGTCTGTTTTTCAGGTC

SEQ ID NO:75 CAACAGTAACGTCACACGGACTA

SEQ ID NO:76 ACACTGTGTTATCTTGGGAAAAATTATTGG

SEQ ID NO:77 GGGACTTTCCCTTTCAGTTTCG

SEQ ID NO:78 CTGATTGTATCGTTGGCAGACTCT

SEQ ID NO:79 AGCATTAGATTCATTTAGAGGGCAACT

SEQ ID NO:80 CGGAATACTACCACCTTCTAGCTGA

SEQ ID NO:81 CAACTTCAGGCTGTCACCAGTA

SEQ ID NO:82 CCTTAATTCTGTTCTGAATTGCTGTCTTG

SEQ ID NO:83 GCTGTTTAGGAGAACCTAGGATAGTTATTG

SEQ ID NO:84 TGTCTACACCTGCAATGAATGCA

SEQ ID NO:85 AGTGAACAGGTATAATATTCAGCTGCTCTA

SEQ ID NO:86 GCACTGCCAGGAGAACAAATG

SEQ ID NO:87 CACACACTCCTATCACCAAGCA

SEQ ID NO:88 TTCAAAGGCCAATCCAACACCAGAT

SEQ ID NO:89 CACGTATTTGGATCTCAGGCAGTT

SEQ ID NO:90 GCAACAGGTGCTTAGTATCCTTCAC

SEQ ID NO:91 GTAAAGGCAGGAAGGACAACTGA

SEQ ID NO:92 CCAGTCTACAGGCCTATCAGCA

SEQ ID NO:93 AGCAGCTAAATTCAATATATCAGTGCTCA

SEQ ID NO:94 CAGCTTTGTTTCATGGTACATCACT

SEQ ID NO:95 CCCAACCAAAATCTGTACCAGGAA

SEQ ID NO:96 GGGTGGAGGTAGTGGGTTTTTC

SEQ ID NO:97 CTGAGGTAGGAGAAGGCAGACT

SEQ ID NO:98 TTCTGTTCTTAGGCCACTTTTCTCC

SEQ ID NO:99 GTCTGACAGCACCTCTTCTGTG

SEQ ID NO:100 CACCTGATGGACCTCCAGAAAAA

SEQ ID NO:101 GGTTGACGCTCTCCACACA

SEQ ID NO:102 GGGCAGTTGGACCTATCTCCATA

SEQ ID NO:103 ATGTACCTCCACTTTCAGAAAGCTTT

SEQ ID NO:104 GTGGACGAGAACCACTCAGAGT

SEQ ID NO:105 TCGGCGTGTGAGTTGATGAG

SEQ ID NO:106 CCAGTATGGCAATGCCTATCCT

SEQ ID NO:107 CCCTCTGCGACAGCTTATAATGG

SEQ ID NO:108 GGGACCCATAAATGTTTTCCTTTTAATCC

SEQ ID NO:109 CCTGAGCAGAGCCTTAGAACTG

SEQ ID NO:110 CCGGGAGTTAGGTTTGAAGCTG

SEQ ID NO:111 CCACTTCACCATCTTATGGGCAT

SEQ ID NO:112 TGATTTCCACGTGGTTGGTGAT

SEQ ID NO:113 ACGATTAAGCTTTTAGATCCTTTTCCTGA

SEQ ID NO:114 GTAGCGCTAAGCACTATGGGTA

SEQ ID NO:115 AAAACAGGGATATTACCTACCTCATAAACA

SEQ ID NO:116 GGCCTTAGGAAGAACTTTCCCA

SEQ ID NO:117 GAGGTCAAATAAGCAGCAGGAGAA

SEQ ID NO:118 CCGGACCTGAAGAACTCGAAC

SEQ ID NO:119 CTGACAGGAAGCCAAAGGGTGAA

SEQ ID NO:120 GTTCTCTGAGGGTCCTGAGTCT

SEQ ID NO:121 CAAAAGTCAGTTATGTCTCTACACTGGA

SEQ ID NO:122 CCCTCAACCATGTATGATATATCTTCCA

SEQ ID NO:123 CTGGAGCCAGGACCATACCAAA

SEQ ID NO:124 CACTATCCCAGGGAATCAAAGAAGA

SEQ ID NO:125 AGGCTAGTAGGCATGGTACTCTT

SEQ ID NO:126 AAGAATTTTTAAAGGAAACTTGACCGTGTT

SEQ ID NO:127 TCTTGTTCACCAGGAAGGGATCT

SEQ ID NO:128 TTGGGCACAAAGGTCAACAGAA

SEQ ID NO:129 CTGAGGTTTGGGTGTATGTAATTTGC

SEQ ID NO:130 GTGAATAACTTTTGCTTGGATCTGTCTTG

SEQ ID NO:131 GTCTGAAACATCTGTGTAAACAGATTTGAG

SEQ ID NO:132 TTTTTCTTCTTTTCTCTCTACGTTTCTCCT

SEQ ID NO:133 CCTGCTCAGTTCACATACTGGT

SEQ ID NO:134 CCCAAGGATTGGAAGAATGAAAGC

SEQ ID NO:135 CTGTTAAATATGCTGGATAAATGACCGGTA

SEQ ID NO:136 CCACAGGCTCTCACTGACCTAT

SEQ ID NO:137 GGCCCAATTTGTTCACTAAAACCA

SEQ ID NO:138 CCTCAGTGGAAAGTAAACACACCA

SEQ ID NO:139 TCAATTCTAGTGGGAGGTAATCCCT

SEQ ID NO:140 GGCTAACTCACCTTTTGCCTCT

SEQ ID NO:141 CAATGATCTTGTGTTAGACTTACTCAATGC

SEQ ID NO:142 GCTGCTACAAAAACAGCCAGTATC

SEQ ID NO:143 GCTTTTTCTTTTTCTCCCTTAGTGCA

SEQ ID NO:144 GGCAGATGATGCTTCAGGACTT

SEQ ID NO:145 GTGCTAGGAAAGAGGCAAGGAAA

SEQ ID NO:146 CCACTGGGTATGGTGGTTTCT

SEQ ID NO:147 TTTTTGTGAAGAGGAGGAAATTTGAGTT

SEQ ID NO:148 GTTCATTTCCCGCTAGTTTAATGGAAAA

SEQ ID NO:149 ATTTGGATTTGAGGTGGATCTCACA

SEQ ID NO:150 AGAGTTGAGAGATATTAGTGAGTTGCTAGT

SEQ ID NO:151 TTCAAACTCGTGTTGTTTGAACTGT

SEQ ID NO:152 GGTCACTTGTCCAGGTCAGAGA

SEQ ID NO:153 CTCAATCAGAGCCTGAACCACA

SEQ ID NO:154 CCAGCCAGGAAGTGATTTCTGAC

SEQ ID NO:155 CCACCTGGCCCAAATATTGCTA

SEQ ID NO:156 CATGGTGCCTGGCTAGAGTATG

SEQ ID NO:157 GTGTCCTTGGCTGCTACTGTTA

SEQ ID NO:158 AAAAATTCACCAGATTTGGTTGTGTGT

SEQ ID NO:159 ACTCATTTTTACTCAAACTATTGGGTGGAT

SEQ ID NO:160 GAAGGCTGGTTTCTCCCATCAA

SEQ ID NO:161 TGTTAGCTTCTTGTAGGTAATGTATCCTGT

SEQ ID NO:162 CCCATGTGCTTCCAGAACTTTC

SEQ ID NO:163 TGCTTGAGGTGAGTTTTTGCATTTT

SEQ ID NO:164 GGAAGACGACACAGGAGTTGATG

SEQ ID NO:165 TGCATTTAAATTTGTGTTGTTCAAGGGT

SEQ ID NO:166 CCGGCTTGGCGTTCTTTG

SEQ ID NO:167 GGCTGCATAGAAGAAAAAGGTAGAGT

SEQ ID NO:168 CATGGGTCTCGCTGATGGATAC

SEQ ID NO:169 AGGGTCAGTGCAGTTAGCTTC

SEQ ID NO:170 CAGGAGCCAAACAATTTCATTGTCA

SEQ ID NO:171 CCACCAGAGAAGCTGTACAGATC

SEQ ID NO:172 CTGTGTGTCCATCTCCCTACAA

SEQ ID NO:173 ACAGCAGGCAACCCACTAATTT

SEQ ID NO:174 TTTTTCTGCCATAGGCTATACTTGGT

SEQ ID NO:175 GGACACACAAGCATCAGTACCA

SEQ ID NO:176 CTGAAACAAAGATTCATGATTGGTACCA

SEQ ID NO:177 CCCAAAAGAGGAGGGTCACTAAC

SEQ ID NO:178 GCCAGTTGGCAAAACATCTTGT

SEQ ID NO:179 CCATTTTCTCCACGGGAACTCT

SEQ ID NO:180 GCCCACACTCATTGCAGACT

SEQ ID NO:181 GGGACAGATGCGATTCCTCA

SEQ ID NO:182 CGCACTTGCCCTTACCTTTTCT

SEQ ID NO:183 GGGACATAGGTGATTCTTCAGGT

SEQ ID NO:184 CGCCAACACGTTTGAACTGAAA

SEQ ID NO:185 GACTCTTCCGGTGGAGACAAG

SEQ ID NO:186 GCTTTGCCTGTGGTGGAAAATT

SEQ ID NO:187 CCAAAGAGCTACCCATTCCAGT

SEQ ID NO:188 TTCCCAGTGGGCTAGGATGTAT

SEQ ID NO:189 CCTACTGCCTGAGGGAAAGTGA

SEQ ID NO:190 CTGGATCCTGACTGTGGGTAAA

SEQ ID NO:191 CCCAGTATACCCATGGTCCTTCT

SEQ ID NO:192 CGCTTCTCGGAAAGCGAAAG

SEQ ID NO:193 TTTCTTTTTGCTAAGGGTGCTACTGA

SEQ ID NO:194 CTCCTCATCTGTACAATGAGCAGT

SEQ ID NO:195 TTGAACTTCTGAAACCACTATCGTAAGAAA

SEQ ID NO:196 CTGCCTGCTTTGAATGACGATG

SEQ ID NO:197 AATCTAAGCAAGGTGGTTTAAAAGTTGC

SEQ ID NO:198 CGTCCTTCCTGGCTCAGTTTA

SEQ ID NO:199 ACACCCAGCTGATATTTTGGGATTTTA

SEQ ID NO:200 CGTAGAGCACCCAGGTCTAGAA

SEQ ID NO:201 GCCAGGCACTGTCCTGATAGATA

SEQ ID NO:202 CTCAGAACCATGTGTTGAGAGGA

SEQ ID NO:203 TACATATAAGGCAAAGCATTAGGTACTTGG

SEQ ID NO:204 GAAGGTAATTAACAAGTATGCGAATGCA

SEQ ID NO:205 GGTTTCTGAAATGTGTATAGCTTGTCAAAA

SEQ ID NO:206 AGTAGGCTCCAAGAGGTGACTT

SEQ ID NO:207 TTTTTGAGCTACTCATGACTTAAAACCTTT

SEQ ID NO:208 CCTTCTACCTGTGCCTTTCCTTG

SEQ ID NO:209 GCAGTTTTAAAATCCTTTTTCTGTATGGGA

SEQ ID NO:210 AGAAGGGACAGAAGAGAAAATGCATT

SEQ ID NO:211 TGTGTTTTTGAAGCAGCATATATATTGGC

SEQ ID NO:212 CGTCTCAAAGGAGGAAGGCAAATT

SEQ ID NO:213 ACCCACTGCAGTATCTAGACAGT

SEQ ID NO:214 TGTTTGTAACAACTTAACCTGTTTCTCCT

SEQ ID NO:215 TCATGCAGACAGAGAGGTCCTT

SEQ ID NO:216 GGGTCCACTGAGACCGCTA

SEQ ID NO:217 GCGCTATGGAGAGAAGGACAAA

SEQ ID NO:218 GCCAACCTGGTACCTAACACAG

SEQ ID NO:219 CTGCAATGAGAGAGGCTGCTAA

SEQ ID NO:220 ATGACTTTGTAACCTTGGCCTGAA

SEQ ID NO:221 GTTTTCACACTCCCTACCCAGAA

SEQ ID NO:222 TCCTTGTCTAGTAACTGAACTGGTGT

SEQ ID NO:223 CCAGCAAAATATTTTCAACTACAAGGGA

SEQ ID NO:224 TGTCTATGAAACTGGGAAAAGCACA

SEQ ID NO:225 GGTCTTGTCCTTGAAGAACATGC

SEQ ID NO:226 CGTCAACGTGATGATTCGGTCT

SEQ ID NO:227 AGCGCACTCCTTTCCATTTCTT

SEQ ID NO:228 CCCAAGCTGCCCTTGTTAGAAA

SEQ ID NO:229 GCTCTTGTCCTAGAAGAGACAAGGT

SEQ ID NO:230 CCCATGGACGTATCTGGTAATGA

SEQ ID NO:231 GGGCACAGGAATGGTTAATGTAC

SEQ ID NO:232 GCTGAAGGATGCGGAGATTGTC

SEQ ID NO:233 TCTCTGCCCTCATTTCCTTTCTTTT

SEQ ID NO:234 ACTGAGTCCAGACGCAGAGATA

SEQ ID NO:235 CTCTCTCTTCTTAAAAGCTACAGTGTTTCT

SEQ ID NO:236 CTTTGGGCATGCTTGTGAGATG

SEQ ID NO:237 CTGGTGCAGACAGTAGGGAATG

SEQ ID NO:238 GTCTTCAGGAAAGGAAAACATTCAACC

SEQ ID NO:239 GTGTGCCCACTGCTAGAAAATG

SEQ ID NO:240 CCAGCCTCCTGTGTCTGTTCTA

SEQ ID NO:241 TGTGGTTTTTGGGAATTTGTAATTTTCTGT

SEQ ID NO:242 TGCTTGTGTCCTACTTCAAAATGTATTAGT

SEQ ID NO:243 GTTGTGTTTTCTTGAAGGCAGTAGAAG

SEQ ID NO:244 GGCCCAAATTCACCAATAATAGAGG

SEQ ID NO:245 GAATCAAGATCACAGTCACACTCAGA

SEQ ID NO:246 CCCAAAGTGTCAGGTTGCAAAA

SEQ ID NO:247 TTTTTCTCCCTATATTAGGCCTTCTTGT

SEQ ID NO:248 AGTGTCAAACTGACAATACCGAATACC

SEQ ID NO:249 GGCAGTAGGTATTTAATTATTTGGGAGACT

SEQ ID NO:250 GAGTACGTTGCTTCTATGATATAGCCAA

SEQ ID NO:251 CATCAACTACCATGTGACTGGCTT

SEQ ID NO:252 TATACGGTTTTCTTTCTACAGAAGAGGAGT

SEQ ID NO:253 TTTTTCAGTGGAGGTTAACATTCATCAAG

SEQ ID NO:254 CATCTCTTCTTCTTTTCCTTCTTAGTCTGT

SEQ ID NO:255 CCTTTGACCAGAATGTGCCTCTA

SEQ ID NO:256 GCAGGTCCACAATACTACTGGA

SEQ ID NO:257 TCTTCTAACGCTGATGCAGCTT

SEQ ID NO:258 AAATTTGAGGCATCTTTTCACTAAAAGAGG

SEQ ID NO:259 CATGGGTGAGAAAGCTCTGTCT

SEQ ID NO:260 CCGCCAAGTACCTCAAGTTG

SEQ ID NO:261 GTAGTGGCTCATCCGCTACATT

SEQ ID NO:262 GCCACAACCCTTACCCTAGGA

SEQ ID NO:263 GGATTCATCAGTAGACGTCCTCCAT

SEQ ID NO:264 GAACTGAGGCCTGAGAGCTT

SEQ ID NO:265 CAGGTGAGCAAGCTCTTCATCAT

SEQ ID NO:266 AGGCTGGATAACTAGGTTGTATCTAATCA

SEQ ID NO:267 CCAGGTCCCAGTCCTTTCTGTA

SEQ ID NO:268 CGTGCTCATGCCTGACTTACAG

SEQ ID NO:269 TGTACCTCTGACAGTGGGCTAA

SEQ ID NO:270 CTGTCTCTTCAGCATGCCAATAAAA

SEQ ID NO:271 GCCTTTAAGTTCACTAGCCAAACTG

SEQ ID NO:272 GAGCTTCTAATCATATGCCTTAAGAGCA

SEQ ID NO:273 GGGCAAGATGACAAAGTTCAAAACC

SEQ ID NO:274 GGCGCTTTGTTTTGTTCGGTT

SEQ ID NO:275 CTGCAGAAAGGAGTGGATCAGA

SEQ ID NO:276 GCGAGCATCACACCAGTACAAT

SEQ ID NO:277 GGCCAGTCCATTTCCCATCAAA

SEQ ID NO:278 GCACACTTACACGTTACATTGGATT

SEQ ID NO:279 CCAGTGCTGAGTTGCACATTCT

SEQ ID NO:280 GCCAACAACACTAGAAATAACTGTTAACAG

SEQ ID NO:281 GTGAAGGTCTCTTTGGCTCTTGA

SEQ ID NO:282 AATCAGGGCAAAAGTCTATGAAATTTTGG

SEQ ID NO:283 CTGTTGTTGCTGAGGAGACAGT

SEQ ID NO:284 GGTGCACAGCTATAGATCTTGGTT

SEQ ID NO:285 GGTCATAAGCACCTGTCTGTGA

SEQ ID NO:286 GTGCTGTGATTGTGTATTACTGAATTCAC

SEQ ID NO:287 CTGTTGGGCCAGAGCTGTATTA

SEQ ID NO:288 CAACAAACATTATGTTTCTGCACCTAGT

SEQ ID NO:289 CCAAGAATTTTCTTTGTTTGGACAACGA

SEQ ID NO:290 GGACATTGACGTCCATGCAGT

SEQ ID NO:291 GAATAGCTAATAGTGTGATCTACAGCAGT

SEQ ID NO:292 GGGAGAGTACTGCTTGCCAT

SEQ ID NO:293 CCAGCTAAATCATCTAAGGCAATCCT

SEQ ID NO:294 CGTAGCCACTGGTCATGTCTTTG

SEQ ID NO:295 TGCTCCAGTTCCTTTCAAGCAA

SEQ ID NO:296 CCGAAGCTGTAGTCCAGGAT

SEQ ID NO:297 CCAGGTTCAGTCCCTTATAGTTCAG

SEQ ID NO:298 CTTCCGCATCAGGTTCCTTCA

SEQ ID NO:299 TACTAAAAAGATGCAGCCATCGAACT

SEQ ID NO:300 CGGTCTGTCTGGTTGTGCA

SEQ ID NO:301 GGTGATGAGAGAAGGTTGAGATTATCC

SEQ ID NO:302 CTTGCCTTTGCAGCCATTGAT

SEQ ID NO:303 TGAGACCTTCAATGACTTTCTAGTAACTCA

SEQ ID NO:304 GCATGCAGATGCACTGGAACT

SEQ ID NO:305 GTAAATCATTTGGAAGCAAAGCTTTCAGA

SEQ ID NO:306 AGAGTGGCTGACCTTGACCT

SEQ ID NO:307 GAAAATACTCACAGCAAATGACATTTAGGG

SEQ ID NO:308 CAGGCATCCTGTATCTTTGCTAATCT

SEQ ID NO:309 GTTACTTTCTGGCAAGACCCTGAT

SEQ ID NO:310 GCTGGCACAGTCATCAATGTTCT

SEQ ID NO:311 CAAAGAAGGAACAGGTATACTTTCAGGT

SEQ ID NO:312 GACACAGGAGGAGGTCACAATG

SEQ ID NO:313 GGTGCTAGGCTGAAGTTTGCTT

SEQ ID NO:314 AATGTGAGCCATAAGACAGGTTCAA

SEQ ID NO:315 CTAAACCCAGCCTCTGTCACAT

SEQ ID NO:316 AATTAGTCTTTGATTGTGAGGGTTCTGT

SEQ ID NO:317 AGAATTCATGGTCTGTTTTCTCATTAGC

SEQ ID NO:318 GATGAGGGACAATACTTGATCAGAGATG

SEQ ID NO:319 CTGAATGAGAAAGAGGAATTTGTGTAACAC

SEQ ID NO:320 CCCAGGATGGTATAATTGGTAGTTCA

SEQ ID NO:321 ACCTTAGGGCTCTCCTCTCAAA

SEQ ID NO:322 GGAAAAGAAGGAAGAGAAGAAAACCTCATA

SEQ ID NO:323 GAGACAGCAGTGCTTAAGGGTA

SEQ ID NO:324 CACACTGGATTTATCCTTTTAAAGTGCTT

SEQ ID NO:325 GGTGACACTTTGTGCCTACTCT

SEQ ID NO:326 AAAGAAAGAATCATCTAGAACAGCAGTCA

SEQ ID NO:327 CTGCAAGACCATAGGAGCCAACA

SEQ ID NO:328 CAGTCAAGCATACTTTGAAGAGCAATATTT

SEQ ID NO:329 TGCAGTTTAGAAATCTTCCCTTTGACT

SEQ ID NO:330 ATCGACACTCTCCATTCAATTCGTT

SEQ ID NO:331 GTTGACCATAGTCCATTTATTTCCTCTGA

SEQ ID NO:332 GTAACAGCAACTGAACTAAAAGGTTGTAG

SEQ ID NO:333 TTTATGCCATTTCAAACTCCTTCTCCT

SEQ ID NO:334 ATCAAGGTAGCTAAACTGATGATGGAAC

SEQ ID NO:335 CAGTGACACCTTTTATCAGAGTAAAGCA

SEQ ID NO:336 TTAACTGACTTTGGTTGCCTAACATCT

SEQ ID NO:337 AAGAAATGATGCTCCGTCCACTT

SEQ ID NO:338 GTCCACAGGCGAGGAGTAGCT

SEQ ID NO:339 GGCCACAGATAAAGATTTTAGTATGTTCCT

SEQ ID NO:340 CACGTCTGACAGGTAGCCAT

SEQ ID NO:341 CACCTTCAGAGATCTTGCCACAT

SEQ ID NO:342 CATGAGGGCACCGTCTGAA

SEQ ID NO:343 TAGGTCAGAACTGAGCTTCTATTCTAGG

SEQ ID NO:344 GCGGAGTGCCATTCAGAAAATT

SEQ ID NO:345 GCAAAAGGAGTATTCTAACAAAACAGTGAC

SEQ ID NO:346 CCTCTCCTAGCAAGTCTCCATTC

SEQ ID NO:347 CAATCGAGAATTTGGAGGAAAACCTG

SEQ ID NO:348 TCACCAAATCAAAGCAAGTTTAAGGTG

SEQ ID NO:349 ACCTATAATATCAGCTGCACAGCCTAT

SEQ ID NO:350 CCAATGAGACAGTTCTACCTGGAGTA

SEQ ID NO:351 CCGTTCATTTCTCAGGATGTGGT

SEQ ID NO:352 GTGTAAGAGGAGGACGCCATTA

SEQ ID NO:353 AACTAAGAGTATGAACTTCCCACATTAGC

SEQ ID NO:354 ACCCAAGCAGACTAATACAGTCCA

SEQ ID NO:355 TTTGCTCTATTTTGTGTCATTCCATTTTGT

SEQ ID NO:356 TTTATGGATTTCTATGGGAACTCAGACAT

SEQ ID NO:357 GCACCAGCCCTATGAACTTCTAT

SEQ ID NO:358 CCGACTTAACTACAATCTTGAAGTGCT

SEQ ID NO:359 CATGCTTTCCCACACAGCTAATG

SEQ ID NO:360 GCCTGTCAGCAGATAATATAACAAACCC

SEQ ID NO:361 TGGACATTATTGGAATGACGCTGAA

SEQ ID NO:362 GTTTGAGTCTGTAGTCTTATTTTCCACAAC

SEQ ID NO:363 CCACAGTTAGATACCACCAGCAA

SEQ ID NO:364 GCAATAGTGGTTATAATCTTACCTTCTGCT

SEQ ID NO:365 CATAGAGAAGTCTTCAAGTCATTCCCAT

SEQ ID NO:366 AACATAAACACTGTAGAGCACATGGT

SEQ ID NO:367 CTGTGAAGTGTGATGCATACTAACCT

SEQ ID NO:368 CTTCTATTTCAATTCAGTTGGGATGCAT

SEQ ID NO:369 CCTTCAGCCCTAAGCCTTCAAT

SEQ ID NO:370 AAGCACTATGAAATAGATGTTCTGGAGATG

SEQ ID NO:371 CCACATTCCAGTGAAAATGTATAAGCG

SEQ ID NO:372 CCCAGGATATCAGTGTCATTAGCAA

SEQ ID NO:373 AATGACTTGCTCCTGAATAACCACA

SEQ ID NO:374 GAACTGCTCCTGTGATGATAAATTATGC

SEQ ID NO:375 GCACAAGAGTTATTGAAACAAAGTCTTCA

SEQ ID NO:376 ATTTTGGACAAAGTTCAGTAAAATCTTGCA

SEQ ID NO:377 ATCCTGCTTTAAGTTTTGTAATACATTGCC

SEQ ID NO:378 CAATTTTGGAAAAGTGTCAGAAGGCAA

SEQ ID NO:379 GAGAAGAGGGAGAAAAATCAGTACCTAGA

SEQ ID NO:380 GCAAAGATAAATTTGGAGCAGAACAGA

SEQ ID NO:381 CGAGGACACCTGGGTAAAGTTG

SEQ ID NO:382 ATGGTTCCAATCAGCTATGTATTAAAGTCT

SEQ ID NO:383 CAGTACAAAAGGTCCAAGAACATGAAAG

SEQ ID NO:384 GCATCAAAGGTATTTTCAGATTGTCACA

SEQ ID NO:385 GGCACAAAATAACCACTTTGACTCA

SEQ ID NO:386 TGCCAGGCCCACTTTAATTGTA

SEQ ID NO:387 AGAAATAAAAATAAGCAACCAAGTGCCAA

SEQ ID NO:388 AGGCAAGGGATTCTGCTGTTTT

SEQ ID NO:389 CATAACAATGAAGCATATGCAAATCACTCT

SEQ ID NO:390 CCCTCCTTTTCCTCCAACTAAATTAACT

SEQ ID NO:391 GGGTCAAACATAGTAAGCTTGGAAAAT

SEQ ID NO:392 CCATGCCTGGCCTAAGATAACTATT

SEQ ID NO:393 TTGCAGACCCTTTCTTAAAAATGGTAAAC

SEQ ID NO:394 AGAGAGCGCAGAGACTCAATG

SEQ ID NO:395 GAAATAAATAGCCCTTTAAGGAGGGTGTA

SEQ ID NO:396 CTTGGGTCTGTCTCCTCAGTTTC

SEQ ID NO:397 AATTTATGACTCACTCACTTCAACACCA

SEQ ID NO:398 CCTGGGCTCTGGATAAGGAAGT

SEQ ID NO:399 GTACTCTACTGAACCATGGCCAAA

SEQ ID NO:400 CTAGGGCACCAGAAGAGCACATA

SEQ ID NO:401 GTCAACCTCACATTTTGGTCCTTC

SEQ ID NO:402 CCACCCAGCGGTTACTGT

SEQ ID NO:403 TGATACAATCATGGGCAAAATAAACATGC

SEQ ID NO:404 GGCCCTGGTTAGGGATAGGT

SEQ ID NO:405 GCTTAGAGTTGCCATTTTACTTCCATTAAG

SEQ ID NO:406 TTCGTGCATGCGAGGTCCC

SEQ ID NO:407 AAATACAATGTCTCACCACAGTGGAA

SEQ ID NO:408 GCGCCTATAAGGCTTGGGC

SEQ ID NO:409 TAGTGAAAGTGAAATTCATCCATTTCTTGC

SEQ ID NO:410 CCCACTAATGCTCATGGCAAAG

SEQ ID NO:411 ACGCAAAGAAAAGTGTCTTTGCAG

SEQ ID NO:412 TTAATCCGGAAGAATAGAATGGCACTTT

SEQ ID NO:413 TTTGCCACATGATGTTTTATTGATTATGCA

SEQ ID NO:414 TGCATTCTAGAAAAATTTGCTTGACAACT

SEQ ID NO:415 CACTTGAAAGCACTTGTGGGTT

SEQ ID NO:416 TATCTTTCCATCGAGGAGGAAATCCT

SEQ ID NO:417 CAGAAAGGAGAATTGGTTTATTTGCACC

SEQ ID NO:418 CTCTCTCTGGGCTCCTTCAGAT

SEQ ID NO:419 TATGGCTGCTACTGCTTTAAAGAGTC

SEQ ID NO:420 ATCCAGAGAAGCGCAAGCTCAT

SEQ ID NO:421 CTGTCTCTTCTTTAACATACCAAAAAGGC

SEQ ID NO:422 GTTTTGAAACAGAGGCTCCTAGGAT

SEQ ID NO:423 GTTAACATCGTCAGGACTTTGTTCC

SEQ ID NO:424 CAATGAACCAACAGGTGCATCTTC

SEQ ID NO:425 ACTTGTCTGATATGGTGAGAAGTTCTGA

SEQ ID NO:426 CACGCTGTCAATTGAAAATGTGTCT

SEQ ID NO:427 CTCTTTTAGCATGTCTAAAATGTGCATGT

SEQ ID NO:428 GCTTTGTACGCTGTCTGGGTAA

SEQ ID NO:429 TACTCTTCTGGTACCTACAACTTTCTGT

SEQ ID NO:430 CTGGATGTGGAGCCTCAGATAC

SEQ ID NO:431 CTTTTGGAACCAAAAGGGTATGGTT

SEQ ID NO:432 GGTGTCCAACAGCTTTGCA

SEQ ID NO:433 TGGCGCTTTATTGTCTCATAAATAGAACT

SEQ ID NO:434 GGCCCAATGTATTATTTTGGGCT

SEQ ID NO:435 TAGGGTGTGCTTCTTAAGCACAG

SEQ ID NO:436 AGGCTGCTTAAAGTACAGAATTATAGTGC

SEQ ID NO:437 TCTGTAGCTTAATTTCTATGCTTAGGGAGT

SEQ ID NO:438 CGTCCGCAGAGGTAGACAAG

SEQ ID NO:439 CCTCTTGGGTTTTGACAGAGCT

SEQ ID NO:440 GGCCCATAGCTAGGAGAGGAT

SEQ ID NO:441 TTGTTTTGTTTTGTAAAATCTCTGGCGTAA

SEQ ID NO:442 AAGTGGGTCACTAGTCTTTGTACTTG

SEQ ID NO:443 GGTTTGTCTTCATGATGTTTCAGGATTT

SEQ ID NO:444 TCGCAAGAGTGGAAGAGAAAGTG

SEQ ID NO:445 TGCTGTACATGCTAGCAATTAATTGC

SEQ ID NO:446 GGAAAGGGACAAAAGCAGTTTGAG

SEQ ID NO:447 GGGACAAAGCAAATGGAAGTCCT

SEQ ID NO:448 TATGTTATGGGATGGGATATTTTCAGGGA

SEQ ID NO:449 ACCCTGAGCATAAAACAAGTCTTGG

SEQ ID NO:450 GAACACCGGTGTCTGAGGATAT

SEQ ID NO:451 CCAACCCTTGTCCTTACCAGAAC

SEQ ID NO:452 TTGAATGAATACCCTAAAACATCCTGGT

SEQ ID NO:453 GCAGTTGAGCCCTGGATTGT

SEQ ID NO:454 TGTTTTTGTAAATTCCTTCTATGGGCTCTT

SEQ ID NO:455 GGACCTATGGGTCCCAGTGTT

SEQ ID NO:456 AATGTTACAGGTAGTACAGAGGGACTT

SEQ ID NO:457 CTCTATTTCAGGCACCATCATGACT

SEQ ID NO:458 AATAACAACTGCTTTACTCTCCTCAATTCA

SEQ ID NO:459 TGACACCTTGCTTGGCTGTTAT

SEQ ID NO:460 AGCTCCTCTTGCTCGCAA

SEQ ID NO:461 CCGCTACCTCGGATGTTCTTC

SEQ ID NO:462 GAGGGTATTGAAAGCTCTCAGCAA

SEQ ID NO:463 TGTCCTGGGCAAATACGCTTTT

SEQ ID NO:464 GCATTCCCACCTACTCAGGATTG

SEQ ID NO:465 ATCTGATTTGATTGACTTTGGAGGGAAT

SEQ ID NO:466 ATAACCCAAATTCAAAGGAACAAGAACCA

SEQ ID NO:467 GTTGTAACATGTACGAATCTTTTACCTCTT

SEQ ID NO:468 CCACCCACCAAGGAAAAACATTTT

SEQ ID NO:469 AGAGGGAAAGGAAAGAGATAGCAAATG

SEQ ID NO:470 CTTTGCCCAACAAAGGGAGAGA

SEQ ID NO:471 GTGAATATCATGGCTATCAAACAGGAGA

SEQ ID NO:472 ATGGCCAAGGCCTTCAAGGAAG

SEQ ID NO:473 GATGATAATACTTTCTGGGTGGTTAATCCA

SEQ ID NO:474 GTGAGGGAAGGCCGACTTAAGGAG

SEQ ID NO:475 AGCTTCAAAATACTGATTTCCAAAGACTG

SEQ ID NO:476 GTAGAGCAGAAGAAGAAGCGGACCTTC

SEQ ID NO:477 TCATTCTTCACTGTTGCCATTGAGA

SEQ ID NO:478 CCACGCGAACTCCTGACTG

SEQ ID NO:479 CTGTGTATATGTAGGCTTTGCCCAT

SEQ ID NO:480 AAAGTGTATTTATTGTAGCCGAGTATCTAATT

SEQ ID NO:481 CGCCCAATGTTATTGACAGCAA

SEQ ID NO:482 GCACTCGCACTGGAACTCA

SEQ ID NO:483 TTTGTTTTGTGTGTAATAGTCCCACTAAGT

SEQ ID NO:484 GCACAGCTTGTCACACCGA

SEQ ID NO:485 CCACCATGCACAGCCATCTTAATTA

SEQ ID NO:486 GTCGTCTTCGTCCTTCGCT

SEQ ID NO:487 TCGTTTCTCCTCGACTCACAGA

SEQ ID NO:488 TGCCTCCATTGACACAAGGTG

SEQ ID NO:489 AACACAAGACAAAAATCCAACAAGCAATA

SEQ ID NO:490 GCAAGCTCAGGTTGCTCATG

SEQ ID NO:491 ATAAGGCCAACCATGATGTGCT

SEQ ID NO:492 TAGGAAAACCCTGGCTCTCAGA

SEQ ID NO:493 GGGAGGTGAAAACAACTCTTCATC

SEQ ID NO:494 CGGTATTCTGTCTGCCCTCT

SEQ ID NO:495 AACTAATAGAACATGCTGCTTTGATTTTCT

SEQ ID NO:496 AAACAGTCTAATGAAACAACTGGTAGCT

SEQ ID NO:497 CCCTGGCAGGTTTTCAACAATG

SEQ ID NO:498 CATAGGTGATGTCGGCATTGGA

SEQ ID NO:499 CCCTGTTGCACTGTCAATGACA

SEQ ID NO:500 AGTCCAGAGGAGCTATGACATTGA

SEQ ID NO:501 CAGTGGTTGACAGAACTCCTGAA

SEQ ID NO:502 CGCTGCATACTCAGCAAATAAACG

SEQ ID NO:503 CAATTGTGACAAAGATACGCACAGA

SEQ ID NO:504 TTAGACCCTCACAGGGCAGAAT

SEQ ID NO:505 CAACGGCTGCCGATTCATAAAC

SEQ ID NO:506 GCGGATTACTCACATCACCTCT

SEQ ID NO:507 AAAAATTGGGCAAATACTGATTCAAGTTGA

SEQ ID NO:508 CCCTCATGAAAAGGTTACAGAGCA

SEQ ID NO:509 ACTGTCATTCTTTATTCATCTCCACATCTG

SEQ ID NO:510 TGGCAGCCACTGTTATGTTGAT

SEQ ID NO:511 CTGTCCAGTTCTGATGGTGACA

SEQ ID NO:512 GCTGTATTTCCTCCTTTGGTCCTT

SEQ ID NO:513 CAGCAATCGGGACATCCAAAAA

SEQ ID NO:514 GGTCCCAGGTGCTTGTCTTTAG

SEQ ID NO:515 TCCTGCCCATTCAAATAAATGGAGT

SEQ ID NO:516 AACTCCAATGATTTCCTCAATGTCAGT

SEQ ID NO:517 TGTATTGAAAAGAATCCTTGTGGATTTCCA

SEQ ID NO:518 CCATGACAGTGGCTGTGTATTCT

SEQ ID NO:519 CGCCAAGCAGCTCTTCTCTATG

SEQ ID NO:520 GCAAAACAGAACACATTAGCAAATTAACG

SEQ ID NO:521 TACATGTTGCCGTCAGTTCTTGA

SEQ ID NO:522 GAAGCACAGACTGGTTTGGTCT

SEQ ID NO:523 CAGCGTCCTCGTGTTAGAAACA

SEQ ID NO:524 TGCCATAAAATAACCACAAAGTTGATATGC

SEQ ID NO:525 GGAAATCTCTTAAATGTTCCTTGAAAGCAG

SEQ ID NO:526 TTTACATCCAAACTGCTTGAAATTGTTTGA

SEQ ID NO:527 CTTTGCCTCTTTTCTGTTCACATAATCC

SEQ ID NO:528 CATAAGAAAAAGGGCCACTAGGTGA

SEQ ID NO:529 AATGACTGACTGTGCATATGTGTGA

SEQ ID NO:530 GTAATTCACACACTCAAAACTCTGAACAAG

SEQ ID NO:531 GAAACACTTCTATTGGATCCAATAGTTGC

SEQ ID NO:532 CCAAGATGATGGCAGTCCCTAGA

SEQ ID NO:533 ACGTAAAGCACTTAGTACAGAGTCATAGT

SEQ ID NO:534 AGCATGCGTGTACATTCAAAATACAAA

SEQ ID NO:535 GTGATTTTTGATGCATCAATACACATCCA

SEQ ID NO:536 ACTTTTTAAAGTAAATGATTTGTGGATAAACCT

SEQ ID NO:537 GGATTTCCAGCTTACAGGGTTGA

SEQ ID NO:538 AATAGTTAGAACATTGTCACTAACTGGTCT

SEQ ID NO:539 GGTTGTTGCTGTCCTTGGAGA

SEQ ID NO:540 CTGCTGAAGCTGCTGTAAAGAG

SEQ ID NO:541 GCACTATCCCTATGGAGGTCCTT

SEQ ID NO:542 GCCCAGCTGGCACTTAACATTTA

SEQ ID NO:543 GTTTAAGGAAAATGCTAAGCAAGTAGTAGG

SEQ ID NO:544 GGGATATGCTGAACTAAGTTGCCA

SEQ ID NO:545 CCGCTTCTGTCCTCTCTACCA

SEQ ID NO:546 CAGATCACCATCAGTATCATTTTTATTGCA

SEQ ID NO:547 ACCTCAAACTCAAGAGCTTTGCA

SEQ ID NO:548 AGAGACTGCGTGCAGTTCTTC

SEQ ID NO:549 AATGTAAATTTATATACTCCATGCACCAATGT

SEQ ID NO:550 CCCTGAAGCCAGAATCGACTT

SEQ ID NO:551 ACTTTCACTGTGAGAATGGTAAAAATCC

SEQ ID NO:552 GAACTGCTCCAGGACAGAGAAC

SEQ ID NO:553 CTGGGTGGGTCTGGTTAAAGTTA

SEQ ID NO:554 CCAGAAAGGCCCTTTCAGGTTA

SEQ ID NO:555 GCCTGGTCCTCCATAGACTCAG

SEQ ID NO:556 GCTGCGAAGTGGAAACCATC

SEQ ID NO:557 CACGACTAGCAAGCAAGTTAAAGAC

SEQ ID NO:558 CGCTTCCTCTCCAGAGTGATCA

SEQ ID NO:559 AAAAACATTGCAAGGAAGTCAAAATGG

SEQ ID NO:560 CCATATTCCATGGGTGGAACCAT

SEQ ID NO:561 TGCGAATAGAAGTGACATAGCATATTGATT

SEQ ID NO:562 CCTGTGTACTTGGGTTATATATTCAGTGG

SEQ ID NO:563 AGCTTTCAAGACATTTTAAGCTTTCATGT

SEQ ID NO:564 GATTTCATTGAACACTTCCTCTCCAAAAT

SEQ ID NO:565 CATTTTCTTCAAGCTTCTGTCTAGTTCAG

SEQ ID NO:566 GTAGGTCTGCGAGGAACAGAAG

SEQ ID NO:567 AGGCTTGACTTCTCCCAACATG

SEQ ID NO:568 GGTTTATGACTGGACAGGGCAAA

SEQ ID NO:569 CCCGTAAAAATAGTGAGACTTGAGTAATGT

SEQ ID NO:570 CGGCGATGTCCAAACTCTT

SEQ ID NO:571 GCTTACCCATTCTTTTTGCCTTTCT

SEQ ID NO:572 ACTAGGATGTAGAACTAAATGCTTGGAA

SEQ ID NO:573 CCTATACTCCTGTCTTTGGCTTTTCT

SEQ ID NO:574 CAACAAGATATGCTTTTCTAGTCATCCCTA

SEQ ID NO:575 CTCAGCATTCGCCAGTCTCAT

SEQ ID NO:576 GCCCAATATCCTCATCAAGCTCT

SEQ ID NO:577 CTCCCATTGTTGATCCTGATCCT

SEQ ID NO:578 TTGTTAGAAACAGCTCTTCCTAAACACA

SEQ ID NO:579 CCCATATATCTGCATCATTGAATGTCC

SEQ ID NO:580 ACTTTCCTCTGAAAGAGCTAAAATTGAGA

SEQ ID NO:581 CTGGGAGAGTGAGAGGGTGTTA

SEQ ID NO:582 CTTCTCTAAATGCCACTGGACAGT

SEQ ID NO:583 AAGTACAAATGAGATTTGAGGTTGAACCT

SEQ ID NO:584 GGGATGTAATCATTGGAGAGGATGT

SEQ ID NO:585 CTCTGGCTGGATTAACCAGGAA

SEQ ID NO:586 ATCAGCTGACACCCAGCTTT

SEQ ID NO:587 GGGAAGTCGTCCTGGTTCATG

SEQ ID NO:588 CGGAAGCAACCCACAGATGTT

SEQ ID NO:589 GGACAAGCCAGCTTCAGAGAATA

SEQ ID NO:590 TGCCCTCTCAAGAGACAAAAACAT

SEQ ID NO:591 GCGTCTGTGTGTCCAATACCATT

SEQ ID NO:592 GGATTCAACCGGAGGAAGACTAAAA

SEQ ID NO:593 CGCCTGGAGAAGTTGTATAGCA

SEQ ID NO:594 CAACCAGGAGCCATTGTCTTTG

SEQ ID NO:595 GTCCACCAAGCATCTGGTTGTA

SEQ ID NO:596 GTATCCTGCAAGTTTACCTAACTGTGA

SEQ ID NO:597 CGTAATGACATGACCTATAATTATGCCAAC

SEQ ID NO:598 ACCAAGTCAGTGGTCTTAATTGAAATTATG

SEQ ID NO:599 CTGATCTAGTCCCTGGAAAGGAGT

SEQ ID NO:600 GGAGCCAGATAGTTTGTATGGCT

SEQ ID NO:601 CCTTGACCAGCTCGTCATTGTA

SEQ ID NO:602 TTGTCCTTTGGTGAAGCTATTTATACATGT

SEQ ID NO:603 GACTCCTGCGTGTCCTTTGTTA

SEQ ID NO:604 TTTTTCATTTCTCTTGCTTACATGAACTCT

SEQ ID NO:605 CCCAGCAGAACTCTCACGA

SEQ ID NO:606 GTACAGTACACCCTCCACCCTAA

SEQ ID NO:607 GCCCTCGGAGCTGAAGAAAG

SEQ ID NO:608 GAAAGAGAAGGCAGAGTCCTGAAT

SEQ ID NO:609 GGAGACCTTGTCTCTCTCAAAAACAG

SEQ ID NO:610 TCCCTTTCTCCCTTTCTCTCGTAA

SEQ ID NO:611 ATGACTTGTGTATCACCTGCTATAACAC

SEQ ID NO:612 ATGCTAAATAAAGGTATCTGCTGAAGGTAG

SEQ ID NO:613 CTGCCACTCCAACGACACCAA

SEQ ID NO:614 CAGACTACTAACCCTCCAGAGTGA

SEQ ID NO:615 AGGTAGGTATCAGCCCACTCAA

SEQ ID NO:616 ACAGAGGCTGGGAAAGGATGATA

SEQ ID NO:617 CAGGCTCAGCAGATGAACATGA

SEQ ID NO:618 AATGTCACTAATAAAGGGATTTGAGGCA

SEQ ID NO:619 GTGGCTTAAATAACTCAGAGGAAGAAAAAG

SEQ ID NO:620 CCCTTCAGTTCTGGAAAACGTG

SEQ ID NO:621 CATGCCAGTCTCATTTCTTGGTTC

SEQ ID NO:622 CATGACTCTAATACCTGCCCTAATCTC

SEQ ID NO:623 CCCAGGACCCTTTGTTTAGCAC

SEQ ID NO:624 GTGCCTTACAAGTCTAATTGCTCAATATG

SEQ ID NO:625 CCTTTCACAGTGAATTCACACTGTC

SEQ ID NO:626 CTGTGTGCTCTTGACAAGATGG

SEQ ID NO:627 GTGTAATTGGGATCCCAGACGAT

SEQ ID NO:628 TCATCAGACTGCAATAGTATACTGAATGC

SEQ ID NO:629 CCCTCGTCCCAAACCTCTCTTA

SEQ ID NO:630 AGAGGTACATAATTTCAGAGGCTTACCT

SEQ ID NO:631 AAAAAGAATGTGTGCAGCTATCAGTC

SEQ ID NO:632 GTTGCCATTCCAAGTGTCTTATTTTTGT

SEQ ID NO:633 GCTAGCAGTCCCAACACAGTTG

SEQ ID NO:634 AGACATGAGTCAGTGTCTATAAATGGC

SEQ ID NO:635 CCCAGTCTCTCCTTGTTTCTCTTC

SEQ ID NO:636 CCCTGTTATACCCAGTTGAGCT

SEQ ID NO:637 GCACCTGGAAAGAGGAGCTTTG

SEQ ID NO:638 TGTTAGGTACTGCCCACCAGAA

SEQ ID NO:639 GGGTACCTTTCCAGAGAGAACTTC

SEQ ID NO:640 AACTCTGAGAAGTTTAAATGTTGGGTAGTT

SEQ ID NO:641 TACCATCCAGATCTAAGGCGTCAA

SEQ ID NO:642 AAGGTTTTGATTCCACATCTGGTGA

SEQ ID NO:643 CCTGGCCTGACACACAATTTTTA

SEQ ID NO:644 AGCCCACTCTGCCAAGTATTATG

SEQ ID NO:645 CGTCCACCCAGATGGTCTTG

SEQ ID NO:646 GGCAAGGTGAGTATGTTGGCAT

SEQ ID NO:647 GAACCTCTTGATGTTCTTCTCACACA

SEQ ID NO:648 GTGCCCAGCCTGATTAGGTAAAT

SEQ ID NO:649 CCCTGGATGGCATTCTGCA

SEQ ID NO:650 GCTGCCGTCAACTAGAACATGA

SEQ ID NO:651 CGGGAGATATACCTCGACTCCTT

SEQ ID NO:652 GATACGAGATCGTGCTGTTCCA

SEQ ID NO:653 CTCATGCCCAACCTTCGTATCA

SEQ ID NO:654 CGGCCTAAAGTTGTAGTTCTTAACCA

SEQ ID NO:655 CTGGAATTTTAATTTCCACGAAGGAAGAC

SEQ ID NO:656 GGATGGCTTAAGCCTGAGAGATAG

SEQ ID NO:657 CCCTCCTCCTACCAACGAAAAT

SEQ ID NO:658 GCCACTGCACCCTGGATTATAA

SEQ ID NO:659 TCTTCTGTGACTCAGGAGTTGGT

SEQ ID NO:660 TTCCATATAGTTATCCATTTTGAACCTGGA

SEQ ID NO:661 CCACCATACTGAACCTGACTCTCA

SEQ ID NO:662 CATGTAAAGTGTGTATGGACAAAGAAGTG

SEQ ID NO:663 GGTGGCAGACTTGGACACTAAG

SEQ ID NO:664 AAAAAGAGAAGAGGAATAAGCCCATTCT

SEQ ID NO:665 GTAGGAGCAGAGCTGAGGAACT

SEQ ID NO:666 GTCCACCAGGCAAGGTCAAAGC

SEQ ID NO:667 TGTGTTGCTGGAGCATCTTCTT

SEQ ID NO:668 CAGCCTTCTCATTAGCCGATTCTA

SEQ ID NO:669 TGTGCTTGCAGAATGTAAAAGATGTTG

SEQ ID NO:670 GCTTGTTCCGATATTCTGCCATGA

SEQ ID NO:671 CACACTATGTCGAAAAGTGTTTCTGT

SEQ ID NO:672 CTCAGATCTACTCCACAGAAAGTGTG

SEQ ID NO:673 CCCATGGAACAAGGGCATTTTG

SEQ ID NO:674 GCTGTGGGCACAGTTCATACTC

SEQ ID NO:675 CATTCTTTAACTGGCCCTCTAGTGT

SEQ ID NO:676 GTTCTAGGCATAACTTTGCCACTAAC

SEQ ID NO:677 TTTTCAACACAGCTTACTCTGTAGCA

SEQ ID NO:678 TGCCATGAAGAGTTACAGCTGT

SEQ ID NO:679 CCGTTAACAACTTGGAAGCTACCT

SEQ ID NO:680 GGTTTCCTCCAAGGAGCTTTGT

SEQ ID NO:681 AGCGGACAAACGCTTAGAACTT

SEQ ID NO:682 CGCACTCGGCCTTAAGGTTAAT

SEQ ID NO:683 AGGTGGATCACGAAGAAGACCT

SEQ ID NO:684 GCAGTATTTTAAGAAGGTCCTGTTGTCA

SEQ ID NO:685 CCACAGGAAGGACCTAACAGTC

SEQ ID NO:686 TTGGAAGTTCACTGGTCTATGAACAAA

SEQ ID NO:687 CCTGGGCCTGCATGGATATC

SEQ ID NO:688 CAATGGTTGTCCTCCTTAAATTGTCC

SEQ ID NO:689 CGGGCTCCTCGAACTACATAGA

SEQ ID NO:690 GATTTTGTAGTTCTGTTAAAGTTCATGGCT

SEQ ID NO:691 TGCAGGTTCATGCTTTTATCAAGAGA

SEQ ID NO:692 TGATTTATTCTGTTTTGTTTGCCACCTT

SEQ ID NO:693 ATGTATTAACTTACTGTGGGCATGATACTG

SEQ ID NO:694 GAATGAATGTTGTTTCTAGGTCCTACTCT

SEQ ID NO:695 ACTTTGTTTGGCTCAGCAGTGA

SEQ ID NO:696 TCAGTTAGCTGTGGAGTCTCTGA

SEQ ID NO:697 CGTGCCCTGGTTCAGACATG

SEQ ID NO:698 GTCTCACAGAGTGATTTATTTTTGTTCTGG

SEQ ID NO:699 GCTATGCCTGTGAGTGCAGTTT

SEQ ID NO:700 TGGGTTGGACAAGTTTGCAATAGT

SEQ ID NO:701 TTTCTACTTGTTTTCAGAATCACTCTGCT

SEQ ID NO:702 TTTTTAATGGTAGAGAGACGGAATGAAGA

SEQ ID NO:703 CAACGTGTGTAGACAGGTTTCAG

SEQ ID NO:704 TGCGGCTCCTCAGGTAGT

SEQ ID NO:705 AAAAATTATTTTGCCAGTAAGCAAGTGGT

SEQ ID NO:706 AATGGTGGGAACAGACGAGATG

SEQ ID NO:707 CGGAGGAGATGGACTTTGAGTACTT

SEQ ID NO:708 GTGTAGTTTGTGCTTTGAGGCTT

SEQ ID NO:709 CAAACATTAAGAAAATGCACAGATGAGACA

SEQ ID NO:710 GCTGAGCATATGGGTCAGTGTA

SEQ ID NO:711 GGTGAGACAGAAGGACCTGTCTA

SEQ ID NO:712 GTCGCACCATGAAACCACCAAT

SEQ ID NO:713 GCTGTTCTGAGTCCCAAGACTT

SEQ ID NO:714 CCCAGCCTAGGAATCCACATTTA

SEQ ID NO:715 TGAGTCTCAACGTCTTACCAGGA

SEQ ID NO:716 TGCTATACTTTGATCAGTGCTCTGTAGA

SEQ ID NO:717 AATCAGTTCACTCTTTAATTCTGCCCTT

SEQ ID NO:718 CCACCTTGGTCTGCTTGTTGAT

SEQ ID NO:719 CCTCAACATGTGGTGGTCGATT

SEQ ID NO:720 GTATGGAACAGCCACTCCTCAT

SEQ ID NO:721 GGTGGTTGGTCCATGAGAGAAA

SEQ ID NO:722 CCCTCACCTTCCCAAGAACTTC

SEQ ID NO:723 AGAGCTGAACAGACTAAGAGGGAA

SEQ ID NO:724 CCACAGGTACCTGGGTAGTCTT

SEQ ID NO:725 CTTGTTCCCAGTTAGTGGTCCAT

SEQ ID NO:726 CTCACCAATAGAACAGCGATAGCA

SEQ ID NO:727 GCGGGACCCTGAAATACTACTT

SEQ ID NO:728 TTTCCTGCTGTTTTTCAGTTGGATTTT

SEQ ID NO:729 AAAGCTAAGTTATGAGCTCTGATATTTGCT

SEQ ID NO:730 ACTCATTTTGTGTAGGAAAGGTACAATGA

SEQ ID NO:731 TCCACAGTATACTATGTAACAGTGTTTCCT

SEQ ID NO:732 AGTATGTCCAAGATCAAAGTACACTGTAAA

SEQ ID NO:733 TGAACAAAACGAAAGGTGAGTAAGACT

SEQ ID NO:734 TTTTTAACCTCAGTACCCATCTTGTAGT

SEQ ID NO:735 ACTCTCCAATGTGACTAGGTTTCG

SEQ ID NO:736 TGCATTATACATCCTTAATCCTCACAACAC

SEQ ID NO:737 CTCAGGCAGGCATACACACACAAT

SEQ ID NO:738 TCCATAGTTCTGTTTTTGATGGCAGT

SEQ ID NO:739 TAGTAATAGGCTCAATTACTTTCCCTCCAT

SEQ ID NO:740 TCAAGAACCTAAGCTTCCAAGTATTCAC

SEQ ID NO:741 CCTCATCTTGTTCACCAGCATGT

SEQ ID NO:742 GCAGAAGTCCAGGCTGAAAAGG

SEQ ID NO:743 CCCACGTCTACTCTGAATGGGA

SEQ ID NO:744 TTTGCAAATTCAGAATTTCAAACTGCATG

SEQ ID NO:745 GCTTGAAGACCACGTTGGTGT

SEQ ID NO:746 TTGTAATAAATGATAGCACTCTCCAAGCT

SEQ ID NO:747 GCAGGAGCACTTGTAGGTGTTG

SEQ ID NO:748 ACTAAGCCCTATTTCTACTCTTTCTACTGT

SEQ ID NO:749 CATCAGGGTGAGGAGGAGGAT

SEQ ID NO:750 CTTGTCAAATTGATGAGTTTCGTCAAGT

SEQ ID NO:751 ACCCTGTTGTTCTGCATATCTTTGT

SEQ ID NO:752 GTCACCCTCCTCATGACAGAAAC

SEQ ID NO:753 GACCTTCTTTATAGCTTCTTCCTGTGT

SEQ ID NO:754 AAGTGGACAGGAATTCAGACATAGC

SEQ ID NO:755 CAGAGGACATGCTGATGAGGATTC

SEQ ID NO:756 TGGTTGAGTGCAGACTATGCAC

SEQ ID NO:757 AGTTGCCTTTGAGATGCTCCAT

SEQ ID NO:758 GCTCCTTCCCAAAGAAGGCTTC

SEQ ID NO:759 TGCCTTCTGCAAAACTAGAAACCA

SEQ ID NO:760 CCAAAGGGAGTCCACCTACAAG

SEQ ID NO:761 TGATCTTGTTGCATCTGACAAAATGAT

SEQ ID NO:762 TGGCAATAAGAAAGTTGGAAAGCAAAG

SEQ ID NO:763 AATTAGGGCTGGCATTGAACTCA

SEQ ID NO:764 CCCAGGCTTTCACATACAATAGGT

SEQ ID NO:765 CCTCTGGCTTCCACAAAGTCAG

SEQ ID NO:766 ACCTGAGTGGATCAGAGTGATGA

SEQ ID NO:767 ATGGTATTTACTTACAATCAAGCCACTGT

SEQ ID NO:768 CCATGCTGCCCACTTAGCATAT

SEQ ID NO:769 AGCTATTGGTGTCATTGGCAACA

SEQ ID NO:770 GGGTGTTGGATGAAGACTGTTG

SEQ ID NO:771 TTCTTACTTTTGACATTCATCCAAGGCTA

SEQ ID NO:772 CTAAGGGAAGGGAGTGGGCAAA

SEQ ID NO:773 CGCAAGTGGAATTTCGATTTTCAGAA

SEQ ID NO:774 GCATCACCCTGGCTCAGATTAG

SEQ ID NO:775 CCAGGAAAAAGATAAAGAAAGAGGTAATGC

SEQ ID NO:776 CACTGGTGTCAGTGAGCATGAA

SEQ ID NO:777 TGAGTCGCACTCATTGACATCG

SEQ ID NO:778 ACTGTCTTGATTCACCTTGACAATAAAATT

SEQ ID NO:779 CACACAGGTGTAAGTGTTGGGT

SEQ ID NO:780 GGTTGGGCAACACATTGTCAAA

SEQ ID NO:781 CACCTCGCTGACTGCGAA

SEQ ID NO:782 GTCAGAAGTCAGCAACTCCTTCA

SEQ ID NO:783 GCACACTCGTAGCCATCGAT

SEQ ID NO:784 CAGACTGGAGAGATGCTTTCTTCTT

SEQ ID NO:785 GGTGCCGTTGTTAAAGCACTTG

SEQ ID NO:786 GTACTCATGAAAATGGTCAGAGAAACCT

SEQ ID NO:787 GTAGGAGCCGACCTCGTTG

SEQ ID NO:788 CATTACTTCTTATCTCCTGAAAAGCTTGC

SEQ ID NO:789 GCGTGTTGTTCTCACAGTGGAT

SEQ ID NO:790 ACCTCCATGTTCAGCACATGAC

SEQ ID NO:791 CCCAGGTCAATTCCTGATTCCA

SEQ ID NO:792 TATGGGTTCCATTTTATGCCAACAAGA

SEQ ID NO:793 CTTTGCTGCTGCTGGATGTTT

SEQ ID NO:794 TTCAGAAGATACTTCTGTGACAAATGAGTC

SEQ ID NO:795 GAGGTGGGCCAGTCTCAAAG

SEQ ID NO:796 CCCTGTTTGTTGCTGTACTTGGA

SEQ ID NO:797 TGCGCACCAGGTTGTACTC

SEQ ID NO:798 AGAGGTACAAGAGTAGAGCTGCAT

SEQ ID NO:799 GCTGGCCTCAGACACTTTGAAG

SEQ ID NO:800 GAAACTTTCTCAGTCTCCATCTCGT

SEQ ID NO:801 GGTATATTGTACAAGTACAGGTTTGTCACA

SEQ ID NO:802 GGGACCAAAAGTAGACATTTGTTGC

SEQ ID NO:803 GCCCAGGAACTAGGAAAGCAATG

SEQ ID NO:804 GGACCCTTTACAGGTGGGAAGA

SEQ ID NO:805 TGTTTGTACTCCAAGTCATCAGAGAAC

SEQ ID NO:806 TGATAACCACTGGTGACTAATGAACAAC

SEQ ID NO:807 CAATTACTAGTACCCTGCATGGTGT

SEQ ID NO:808 GCTGCTGCATCCTAAGCCAAATA

SEQ ID NO:809 ATCTGGAATCATGTTGGTGCCTT

SEQ ID NO:810 TGGGTTTCTGCTAGGTTGTCTG

SEQ ID NO:811 GCTCACCACTTGTTTTAGCCGT

SEQ ID NO:812 TGCTGCTGAGGACTTAAGTGC

SEQ ID NO:813 AGTTTCTTCTGCTTCTAGTCTTCAGAAAG

SEQ ID NO:814 GCTTTACCTCTCCTGGTTCAGT

SEQ ID NO:815 AAATGTGACTAATGGTATGTAACAACCAGT

SEQ ID NO:816 GGACCCAGAGCTTGGTTTGTC

SEQ ID NO:817 TGAAGCAGTTTTGGGATTTGTTTCTTT

SEQ ID NO:818 AATTAATGCAGTGAGGGAGAAAAGGA

SEQ ID NO:819 CAGGTAAGACCTTAGCTTCAAAAGAATTG

SEQ ID NO:820 CCCATCCTCAGGTCCTGTAAATG

SEQ ID NO:821 TGAACCTGAAAAATAGAGGATGGCTAAAA

SEQ ID NO:822 CATCCACCTGGAGAACAGAGAC

SEQ ID NO:823 TGCAGTGGATCTCTTATAAAATTCCATGAA

SEQ ID NO:824 ATCAATATCAGACTACAAAGTGGACTGAA

SEQ ID NO:825 GCTCCCAGGGTTTAGGACTGAT

SEQ ID NO:826 ATTTTTAAGGTGCCTTAAGTCCTTTTAGGA

SEQ ID NO:827 GGGCCATCTCCAGAAGACAATG

SEQ ID NO:828 ATAAAGGCACCATATATTGTGTTTGGGA

SEQ ID NO:829 GCACGGCCTCGATCTTGTAG

SEQ ID NO:830 GACATTTTCTAATCCCATACCCATGCT

SEQ ID NO:831 CCCAGACCTGGAGCTTTCTTTC

SEQ ID NO:832 ACAGAAACATATGTGAAGAACAAGGGT

SEQ ID NO:833 CAAGATGGCACAGCCCATAAGA

SEQ ID NO:834 CTTATCTGCTGCAAGTGACCCTT

SEQ ID NO:835 AGGAGACTGGAATTCTCGAATAAGGATTA

SEQ ID NO:836 TCATCAACATCAAAGATACCTGTTTCTGTT

SEQ ID NO:837 CCTCTAAAAGATCCCAAAAATGTTTCTCCA

SEQ ID NO:838 CTTAGGTTATGGAAACCAAAATATGCAGG

SEQ ID NO:839 AACACCCAAGTGTCCCATCAAT

SEQ ID NO:840 TTAAAGTCACTTTTAGAGCCCTTAATGTGT

SEQ ID NO:841 TCACGATGTTCTAAAATGAAGGAAGAGTT

SEQ ID NO:842 GCAAGCCTGATGGAACAGGATAG

SEQ ID NO:843 CAAAATCAAAATGGAAGTTAACTGGCTGA

SEQ ID NO:844 AACAAAATGAAATGAATGGTGCTTACTTCA

SEQ ID NO:845 CCCAGGAATAAACAGAACACATTTCAG

SEQ ID NO:846 CACCAAGCGGAATCCCATCTAAA

SEQ ID NO:847 GGCACAATATACCCTAATGATAATCAGGAA

SEQ ID NO:848 GCACCATGCTGATTTCACAAAACA

SEQ ID NO:849 AAGATTCTATCCTCTGCCTCTAGCAT

SEQ ID NO:850 CCCGCAGTGTCTCAGGATTTAC

SEQ ID NO:851 AAAATGAAAGCAAATCTCTCTCTTCAAAGT

SEQ ID NO:852 GAGGCATTAGTCACTTGGCTTGA

SEQ ID NO:853 CCAAAGTGTGATCCATAGATGTTACTAGG

SEQ ID NO:854 GGTAAAAGCCAGGACTTTGACAGAATA

SEQ ID NO:855 AGGTTAATTACATTTCAGTCTGTGCCAT

SEQ ID NO:856 CCCATAGCACTGAACCATCCAA

SEQ ID NO:857 AGTGTTTTCCTTTATTCTGTGACCATCT

SEQ ID NO:858 ATCAACCACCTAAGGACAGCAGAA

SEQ ID NO:859 CAGAAAAACTTGGTGAAATTCAGAACCA

SEQ ID NO:860 TGAGTCATATTTAAAGGGAAAAGCACTGA

SEQ ID NO:861 CAGTCATCTTGCCCATTACATCTTAAGT

SEQ ID NO:862 GCCTTAGGGAAAGGTTAGATTCTGAAC

SEQ ID NO:863 TCATATTCCCTTAGAGCAGACCATCA

SEQ ID NO:864 ACTATTCCAGAATGTCAAGAAGTTGCA

SEQ ID NO:865 AAATGGTAAATCCTACAAGGGTAAAGCA

SEQ ID NO:866 ATGATGACCTATGCAGAAAGAAAATGTCT

SEQ ID NO:867 ATCTCAATGTGTCAGGAGCAAAGT

SEQ ID NO:868 GGACAACAGTCAAGTGGCAAGT

SEQ ID NO:869 AGTTGAAAGAAAAGGCAGGTTATAGGTT

SEQ ID NO:870 ACAGTATTTTGTGTCTGGTGTTGAGA

SEQ ID NO:871 TGCCCAGCCGAGAAATCTAAATT

SEQ ID NO:872 CAACAATGATCAGCAAAGAGAAGGAT

SEQ ID NO:873 GAAGATTTCCAGATGGCTGGTCAT

SEQ ID NO:874 CCCAAACTGAGTCTTGCCATAGT

SEQ ID NO:875 GGCCCAGGACTAGAAAAGGATATAG

SEQ ID NO:876 CTGGTCTGTGTGGAATGCTGAT

SEQ ID NO:877 TACCTTATATCCTTCTGGGAACATAGCA

SEQ ID NO:878 GACAGTCCTGCGACGAACTT

SEQ ID NO:879 CTGTGACTTCAACCACATCTATACAAATCT

SEQ ID NO:880 CTGGTTTTTGGTCTTCAGCATTTCATT

SEQ ID NO:881 ATTTTGCTAATTGAATACAAAGTGGCCAA

SEQ ID NO:882 CACCTCAATGGCCTAAGCAAGT

SEQ ID NO:883 TGTATTTGTACATGTATGGTGTGTACTTGT

SEQ ID NO:884 ATGAACCGGAAAACCCAAGAAACTG

SEQ ID NO:885 ACCAGGATCTCCCTCGTCTTTT

SEQ ID NO:886 CTACTTTAGCTTTTAATCCTCCACATGGA

SEQ ID NO:887 TGTCTCAGGTGGATCTTGGTGT

SEQ ID NO:888 CCATACTACCTGTAGAACTGAATTCTGC

SEQ ID NO:889 CCCAGGGTAAAGTTACGGATTG

SEQ ID NO:890 TCCTCACCTAAGAAATGGAGATAATAGTCC

SEQ ID NO:891 GGGTACTGAGGGTCAATGGAGA

SEQ ID NO:892 GTGGCTACACAGAGCAGGTTT

SEQ ID NO:893 GACAGAAACTGCCTTCCACCTT

SEQ ID NO:894 CATGGCTGAATGCAAGGCTTAG

SEQ ID NO:895 GTGGCTGCTAAAGAAAATGTAGATGG

SEQ ID NO:896 CCCAAGTGTCCCTGATCTATGG

SEQ ID NO:897 TAACACAACACAACAAGGCTCTGT

SEQ ID NO:898 AGCTGCCTCCGTAACATTTCTC

SEQ ID NO:899 GTGAAATTGAAACCACCCTAATGCA

SEQ ID NO:900 CTTGTGTTTGATGTTGAGGCAGAA

SEQ ID NO:901 CCCAGAGCAGATGAATCATCAATAGT

SEQ ID NO:902 CCACTCCCTACCTACTCTCTGA

SEQ ID NO:903 TAACACAATTTCAGTGATGTGTGTGGA

SEQ ID NO:904 CCATTTAAACTATTTTCTCTGCCACACAT

SEQ ID NO:905 CAATTCCTCTGGGATGAGACATTTCA

SEQ ID NO:906 ATCTACTCCTTCCACTGCGAGTA

SEQ ID NO:907 ACATGATACTGACATGTTAGGACACTTTC

SEQ ID NO:908 CCCTGTTCATGCTCTTCATCTTCA

SEQ ID NO:909 CCCTGTGATCTCACAATTTAGAAGTCT

SEQ ID NO:910 TTTGGTTTTGTCATACTTTGACCTTTGTC

SEQ ID NO:911 AGCTATTTACCTGCATGGAAATCTATTCC

SEQ ID NO:912 CCCAACCTAAGCACTGTTAGTCAG

SEQ ID NO:913 TCCTGATTAACTTACATGTCTCTGGGAT

SEQ ID NO:914 CTCCAAGGCAGACACCTACACCA

SEQ ID NO:915 AGGATCCATATGTATAACTGAGGTTGGA

SEQ ID NO:916 CTTCTTAAAGGTAGGAGCCATGGTT

SEQ ID NO:917 CGGAGCTGCAAGGACTTAAGTT

SEQ ID NO:918 TAGATCTCATGGCATAGATTTTGCATGAG

SEQ ID NO:919 CCTGTGGCTAGAAGTACAAATAAAAGC

SEQ ID NO:920 ACAGTTTTAATTTCAAGAAAACATGGCCAA

SEQ ID NO:921 TGAATCATTCTAGGAAGAAGCAACAGTAC

SEQ ID NO:922 AATCATAAAAACTGCAATGGGCACAA

SEQ ID NO:923 CCCAGAGTTAGCATCCCGATT

SEQ ID NO:924 AATCAGATCATCAGCACTAGGAAAGTTC

SEQ ID NO:925 CAAGAGTGAGGTCCCTATCTCCT

SEQ ID NO:926 AAAAAGAGCACATTACGTATTCAAGACC

SEQ ID NO:927 CCTGGCCCTTTCAGCTAATGAC

SEQ ID NO:928 ACATACAGCTTTCCAACAGCCAT

SEQ ID NO:929 CAGCCTTCGGTCTCTTCGA

SEQ ID NO:930 GCAGAAAGCAAATACATCAGGTACCA

SEQ ID NO:931 GAAAGTCACAAATGAAAATAGCAAGGACA

SEQ ID NO:932 TGAGAAATCATTTTTCTTGAGGCAGAGTT

SEQ ID NO:933 CTAATGCCTAATGGTGCTACAGTTTC

SEQ ID NO:934 CTGAGGCTTTATCTATATTTTGGGTGGTT

SEQ ID NO:935 GGGCAAAATGTAATTTCAAATTTTGTGTGT

SEQ ID NO:936 AGTTCCACCAAGCAAGTGCTTA

SEQ ID NO:937 GGTTTGAAAGTCTTCCACTAAGATGCA

SEQ ID NO:938 ACTCTTACTGCTTTATAGTTTGGTTGCT

SEQ ID NO:939 GTGGTCCAGAAGGCTGTATCTC

SEQ ID NO:940 GAGCTGTTTAGAGGTGATAGCCT

SEQ ID NO:941 TCTGCTCTGTAAACATCCTTTTCAAACT

SEQ ID NO:942 TTTTTAAGCAGATTCATGTGTCAGTTCTT

SEQ ID NO:943 AGAAACCTTTGGATAAGAAAGAAAAGCAAC

SEQ ID NO:944 CACAGTGTCTACTTAGATAAAAACACTGGA

SEQ ID NO:945 TTGGCTGTCTTTGTCAGAAGTCA

SEQ ID NO:946 GCGTGAGCCACTATAATCCCAT

SEQ ID NO:947 TTTTATGCTTTGATGCCGAATATGTGAATT

SEQ ID NO:948 TGTGTTTTCATCGAGAGCACAGT

SEQ ID NO:949 CTTTTTCATTCTAAAGCAGTATTATCTCACACA

SEQ ID NO:950 GCATTCTTGGGAAGCTTTTTAATATCTGG

SEQ ID NO:951 TAGCTTTGAGTCTTTCTACATGGAAGAAC

SEQ ID NO:952 GTTTGTCCATCATATCTCACTTTTCTTCAC

SEQ ID NO:953 TCATCTGAACCATCAGCACAATCA

SEQ ID NO:954 TTTTTGGTAGTGCAAGGATTGAGTC

SEQ ID NO:955 AGGTAGCAGAAAGCTATGGTGTTTG

SEQ ID NO:956 GGGCTTCAGAAAGCTTTAAGATGATTTT

SEQ ID NO:957 TCTAGTGGAACAGGCACCAGAT

SEQ ID NO:958 AGACTAAGAGGTCAGGGTTCGTT

SEQ ID NO:959 CGAAGTCCTTTGGAAACGAATCG

SEQ ID NO:960 TGAGTCCCTTGAGGCTATTTGC

SEQ ID NO:961 GTTAATAGTTTTCCGCCTGTCTGAGA

SEQ ID NO:962 GTATGAACCACTGCTCTGGGAAA

SEQ ID NO:963 CAGGAAAGAGGAGTAAACACATTACTGA

SEQ ID NO:964 ATTAACCCTCACAATGCACTCTGT

SEQ ID NO:965 GTTCTGTTGGTTTTTGTGAAGATTTTTGG

SEQ ID NO:966 GGTTTTCTGGGAAGGGACAGAAG

SEQ ID NO:967 GTAGGATTCATCTAGGAGACAAATGCAG

SEQ ID NO:968 GCGATTTTCCCGAGCTGAAAAT

SEQ ID NO:969 ACATTCATGGGTTTTACATTATAAGGGCT

SEQ ID NO:970 GGCCACACTAGTGTGTCTGATC

SEQ ID NO:971 TGAAAAGTTGTATAGGCACAGATGTAGTTT

SEQ ID NO:972 CCTCAGTCACTGTGTTATGTCTTCG

SEQ ID NO:973 AAATGAACATGATGGGATTTTCAACTTTGT

SEQ ID NO:974 CCCTGAACCTGAAGGCCAATG

SEQ ID NO:975 CATTTAGTCTTGGGAGTCCCAACT

SEQ ID NO:976 GCTGGGTCCAGGAATATGGTAAT

SEQ ID NO:977 AAAGAGATGCTTTATGTAGTTCTGGTCTTC

SEQ ID NO:978 CCCTTTTTGTTTTCCCTTCTATTTAAGAGC

SEQ ID NO:979 AGGCACAGCCAAGTTATCCAG

SEQ ID NO:980 GAAAAATAACTCATTGGCAATTCAGGGA

SEQ ID NO:981 GTGGAGGAGAAGCCACAGGTTA

SEQ ID NO:982 GCCTGCTCCCATCCTACGA

SEQ ID NO:983 TCTGAGCAGGATCCGGCTCC

SEQ ID NO:984 ATGGAAAGAATAATTACCATCAGGTTGTCT

SEQ ID NO:985 GTTTACTTGAAGGCCTCCGGAAT

SEQ ID NO:986 CCTAGAACTGATGGTACGCAAGTTC

SEQ ID NO:987 AGAACAGTCTCTGATGAGGATGCT

SEQ ID NO:988 GAAAAATGGACAGTATATATTTGCAGGCAA

SEQ ID NO:989 GCAGTCTTAAACTCCAGGATGCAA

SEQ ID NO:990 TTTGTTCAAGTCAAGATGGACAAACTT

SEQ ID NO:991 GCAACCCACTAGCGAGCTAG

SEQ ID NO:992 TATGACTCATTGCAGCTCGACTTT

SEQ ID NO:993 CAAAATTCTCACTTCCCTTTTTCCTTGG

SEQ ID NO:994 ACTGGACCAACCTTTGTGTCTATG

SEQ ID NO:995 AGATCTGCGACAGCGTGAG

SEQ ID NO:996 ACACATTGAACACTCATTACAGAGACAA

SEQ ID NO:997 CCCAGACCTACCTGTTTCAGG

SEQ ID NO:998 AAAGTGTGCATCACTGTAAAAGATTCG

SEQ ID NO:999 CTGCAGACTCAGGAGAGGAGGA

SEQ ID NO:1000 ACATGAGCACATCTGCCAAAAATATAAG

SEQ ID NO:1001 GCTGGACATCTCGGCGAAG

SEQ ID NO:1002 GATAACTGGACGGAGGTTGAAGT

SEQ ID NO:1003 CCAGGAGCTTTTTGGACTATGC

SEQ ID NO:1004 ATAAAAGAGCAGGATGAACAAAATGGC

SEQ ID NO:1005 GTCACTACAATGGACAGTGACAGG

SEQ ID NO:1006 AACATGTTTCAGCTACGGTCACTATT

SEQ ID NO:1007 TCATGTCCGGGTCCTCGTAA

SEQ ID NO:1008 TCTTTTCCATCAAGGTCCTCAATAGC

SEQ ID NO:1009 GGCAACGGTCACCTGATCTG

SEQ ID NO:1010 TTCTGCACAGTGTTCTTACGATAACA

SEQ ID NO:1011 AATCAACACGGACAAGCTCTCA

SEQ ID NO:1012 ACTGAGTTTCCCATTTAAGCACCA

SEQ ID NO:1013 TTTTAAATTTGGCTCCAAGGAAAGCA

SEQ ID NO:1014 TGGTACAGCTACCAAAGGGTATGT

SEQ ID NO:1015 GCACTGTGAAGAGTTGCAAGTG

SEQ ID NO:1016 TCCACTTATTATTGAGTAGGTGATTTCTGC

SEQ ID NO:1017 TCATCCTCACTCACAGTCCCTTTAT

SEQ ID NO:1018 TCGGATGAGATCTATCTCTGTTCCAA

SEQ ID NO:1019 GGTAAGTAACGTGACCATAAATACCAGA

SEQ ID NO:1020 CCAGTGCTGCTGTCTACATGAAA

SEQ ID NO:1021 ATTGCTTTTTCAGTGAAATTTCACCCA

SEQ ID NO:1022 CCATCGGAAGCTCTAACGGTTA

SEQ ID NO:1023 CGTTGTCATTCTCATCCTGCAAGT

SEQ ID NO:1024 GCAGATACATAGAAATGCTTCAATACCCA

SEQ ID NO:1025 TGCTGGTGAACAGCTTCATGAT

SEQ ID NO:1026 GGGTTCTCTACAAGTACTATTCTCACCA

SEQ ID NO:1027 GCTGCTTACCTTTTCACAAACTGG

SEQ ID NO:1028 GGGCCAATATGAGGATTATCAAAATCTG

SEQ ID NO:1029 GTGGTCCAAGAAAAATGAAAATATGGTGA

SEQ ID NO:1030 AGGCCCTTTTGTTTCCATCTTCA

SEQ ID NO:1031 GTTTCCATATCAATCATGAAGCAACTATCC

SEQ ID NO:1032 ATTTTCTCAAACAAAGGTGTGTTGTCATT

SEQ ID NO:1033 GACTCTTGCGATACTGGTCCTT

SEQ ID NO:1034 GCCGCTACAAAAACTAGATTGATTTTACT

SEQ ID NO:1035 GAATTCGGGATGCTCTGGGATA

SEQ ID NO:1036 CAACACTGACTGCAGTGTCTGA

SEQ ID NO:1037 GGGAGAAAGGGAAAAGATAATTAACAGACA

SEQ ID NO:1038 CGTCGTGAACTTCCCATCTGTT

SEQ ID NO:1039 CCCTAACAGAAAAGCATAAGCATAAACA

SEQ ID NO:1040 TTGTGCCCAAAACTCATCAAACC

SEQ ID NO:1041 AAAACCTCTTGGACGAAACCACT

SEQ ID NO:1042 ATCCACAGACATACTTTAGCTTTCAAGAAT

SEQ ID NO:1043 CCTCTTTCTCCCAAAAGATAACCTGT

SEQ ID NO:1044 AAATCAATGTGCTGATTAATTTGGTCTCA

SEQ ID NO:1045 CACAGATTAGAAGTATTTGGATTCAGACCT

SEQ ID NO:1046 AGCCAAAAGTTCACAAAATTTATTGGGAA

SEQ ID NO:1047 CTACCAACGTTTTTCTTACCCAATATTCC

SEQ ID NO:1048 AAAGATTATGGCTTATCTTTGATACGTCGT

SEQ ID NO:1049 GAAAGACACACATAAAAGCACTTATGGATC

SEQ ID NO:1050 ATTCATTTATTTTGTCCTGGACTCCATCA

SEQ ID NO:1051 TCTAAACCCAACAACTCTGTTACACTG

SEQ ID NO:1052 GTATTCAAATTTTTCCATGACCACTCTGT

SEQ ID NO:1053 CTGCCACAGCAAATTAACATTCAAGT

SEQ ID NO:1054 GCCCACAGAGGACCTTGATG

SEQ ID NO:1055 TTCCCAAAGTACCAGTTTGCCA

SEQ ID NO:1056 AAAGAGACTGACGATTTACCAATTATTCCA

SEQ ID NO:1057 GCCAAATGTATAACTCAGAAGGAATCATGA

SEQ ID NO:1058 AAATGGGAACTTTTCAGCAGATAGACT

SEQ ID NO:1059 GGTTCTCTTTGTGAGGGCACAT

SEQ ID NO:1060 AGATCCACTACTCACAATTCTATCACTGA

SEQ ID NO:1061 TGAACTGCTCTGAGGAGATCGA

SEQ ID NO:1062 GCAGAAAAGGAATCCTTAGTGAACAC

SEQ ID NO:1063 GCAGTTGTGCTCCTGAAGAAC

SEQ ID NO:1064 TGATACTGAAGAATTGATGGCAGTGATG

SEQ ID NO:1065 CATCGGGCACCTGAACGT

SEQ ID NO:1066 CCATGAAAACATTCTTCCACTTTAGTCTG

SEQ ID NO:1067 CCTGTCCAAAATGATTCTTTCAAGCAG

SEQ ID NO:1068 TCCAGGTTCCACCTTAATTGGC

SEQ ID NO:1069 GGCCGTTAGCAATAGGAACTACC

SEQ ID NO:1070 CACAAGCATCGGTAACTTGACAAAAA

SEQ ID NO:1071 GGAGGTATGAGTCAGTATAACTGTGC

SEQ ID NO:1072 CTGTTGCTGTGGCTGAGATTTG

SEQ ID NO:1073 TAAAATGCGAACTGATCGTCCCTT

SEQ ID NO:1074 AGTACATGGACAGGCAGACAGATA

SEQ ID NO:1075 GGAATTATAGGCATGAATCCCTACACATG

SEQ ID NO:1076 CCCTGGGAATAGCTTCAGGAAG

SEQ ID NO:1077 GCCTTGCTCAGTAGTTTTCATTTTAATGAA

SEQ ID NO:1078 ATGTCCCTTGTGTGGGACTAAAG

SEQ ID NO:1079 GACCTACTTTGGCCTGAGCTT

SEQ ID NO:1080 GCTCTCCTGACTTAAACTTTTAAAAGTCAC

SEQ ID NO:1081 GTTGCTTAGTTTCTCATTTCCATTTCTGTT

SEQ ID NO:1082 CGTGGGCTTCTCCCTTTTACTT

SEQ ID NO:1083 GAACAAACAGGATTTTCAAAATACCAGGTA

SEQ ID NO:1084 GGGAGACTGTCTTCTTAGAGTTGATT

SEQ ID NO:1085 CCCTTTTTAGTAACGTGACATCAGAATAAG

SEQ ID NO:1086 CACAGTACCGAGAAATGTTACGGA

SEQ ID NO:1087 GGATGCAGAATATGCCAACTACTATACTAG

SEQ ID NO:1088 GGGTGAACATCAACAACAGCATG

SEQ ID NO:1089 CTTATAAGACCGCATCTTAAAGGACTTTTT

SEQ ID NO:1090 GCTGCATAGGCTCTTTCTATGGAG

SEQ ID NO:1091 CCACATGCAAACTGAAGATCGAACTAT

SEQ ID NO:1092 CAAATCCCAGCATATATAACAGAAGGGT

SEQ ID NO:1093 CTTCAGCCTTTCTGAAGAGTAGTAAGTTTT

SEQ ID NO:1094 AAATTTGAAATGAGTGGCTATACTGTTTGG

SEQ ID NO:1095 GAGAGAAATCGCCCACTTTCTGAT

SEQ ID NO:1096 CAAAGACATCCTCAAACCGAGGAA

SEQ ID NO:1097 TTCTTAAACAGTTCGTCCCTTGATTAACA

SEQ ID NO:1098 GCATTTCCAAACCAAACACACTAAACA

SEQ ID NO:1099 CCTCCACTCACTCCACTCTCTA

SEQ ID NO:1100 GGCCAGAAATCTTGCCGTTTTT

SEQ ID NO:1101 CACCTAGAAACCAGACCTTCACTTA

SEQ ID NO:1102 AGTACTTCAGTGTTTTCCATGGTTTTACTA

SEQ ID NO:1103 GGCTTTTCTGACACTCCAGGTA

SEQ ID NO:1104 AGGACCCTGATTTGGTCCACTA

SEQ ID NO:1105 GTACCACATGAAGCCAGTGAGT

SEQ ID NO:1106 AACAGAACTTGTGCTATACAGCTGT

SEQ ID NO:1107 GCGGATGATCTGTTTGTTCTCCT

SEQ ID NO:1108 CACTTTGGATGGTTTCTTATCTAGGCT

SEQ ID NO:1109 ACAACAGGTTCAATCTTGGCTATTAATAGA

SEQ ID NO:1110 CAGCCTTTAAGTTCCTTGAATTCAAGAAAT

SEQ ID NO:1111 CAATGCCATTTTTATTTCCTCACTGATTTC

SEQ ID NO:1112 GGCAGCACCCTGTCTGTTTAG

SEQ ID NO:1113 CCTTCTCCTTCTTGAGTACTTTATGGAATT

SEQ ID NO:1114 CATCGTCAACCTGGAGAGCAA

SEQ ID NO:1115 GCCATTCCCTAAATGTGGATTCCTAG

SEQ ID NO:1116 CAGCTTTCAGTCCTGACCTATTGA

SEQ ID NO:1117 GCTTCTTAGACTTCAACACGTTAGATTAGT

SEQ ID NO:1118 CCTATTCCAGGCTCCGTCCTAA

SEQ ID NO:1119 ATGTCTGTGTGATTTTAATTCAGACACAA

SEQ ID NO:1120 TGAATATGACTTGGAACTCTGAGAGGTTA

SEQ ID NO:1121 TCTGTTGTAAGACGGTCTCAAACTATTTTT

SEQ ID NO:1122 GTCACAGACTGGACCTGGTATC

SEQ ID NO:1123 CTGGTTGTAGCTAACTAACTTCAGAACA

SEQ ID NO:1124 ATAAAAACAATTTGCTGCAGGGATTGT

SEQ ID NO:1125 GTGGGCATATGAATCTGTCTCCT

SEQ ID NO:1126 GAGGAAGTTCATTAGTTAGAACCTACAACA

SEQ ID NO:1127 CTGAAAGGGCCTTTCTGGTCA

SEQ ID NO:1128 GGGCCATCATGTCTTTTTGTTTGA

SEQ ID NO:1129 GCTTTACGGGCTTGTAGGTGATG

SEQ ID NO:1130 TGCTATCCCAAAATGTCTTTAAAGCTACT

SEQ ID NO:1131 TTACGAGGACCCGGACATGAAG

SEQ ID NO:1132 AGCAGCCAGGATTCCGTTTTAA

SEQ ID NO:1133 CTTCTGGTATCAAAATGCTCCGGA

SEQ ID NO:1134 CATCCCGACCATCCATCAGATC

SEQ ID NO:1135 TTCTGCACACATTTGAAGTAGGACA

SEQ ID NO:1136 GTTAAATGACAGAACAAAGAGCAGCAA

SEQ ID NO:1137 TTAGAGGCCACGAACATGCAAGT

SEQ ID NO:1138 TCATGCAAAATCTATGCCATGAGATCTAAT

SEQ ID NO:1139 GAGGCTCCTGTCACCCTTTATG

SEQ ID NO:1140 AGTTATTTTCTTTGCAAAGGCAAATGGTTA

SEQ ID NO:1141 ATGAAGGATGTCAACATAACGTGTCTTAT

SEQ ID NO:1142 GAAGTGAAGGCTGCACTTGTTG

SEQ ID NO:1143 CTGGGCCAAGACCCTGCAAAAT

SEQ ID NO:1144 GGCCTAGTTTCTCCATTTTGTGGT

SEQ ID NO:1145 GCCTCTGTATGATCTTAGATTCCAGAAATC

SEQ ID NO:1146 AAGTAGGGTGGCATGCTATTGG

SEQ ID NO:1147 TTTTTCTCTGCAGAAATGTGTGTACTC

SEQ ID NO:1148 AGGTTTTGTAAGGCTTGTTGAGACA

SEQ ID NO:1149 TCTCCTCCAGGAATCTACACTGTAG

SEQ ID NO:1150 ATGTCTCGGAATTGTGAAGGCAT

SEQ ID NO:1151 GGCCTGAAGAAATGAATGCAATTTCT

SEQ ID NO:1152 GCAAGCTGAGAAAGCATTGAATTCTG

SEQ ID NO:1153 CAGTTTAGATTTACTTGCTCATTGGTTGAT

SEQ ID NO:1154 TCCAAAGAGCATTTGCCTTTTCC

SEQ ID NO:1155 TCTGTGCTAGAAGCCAGAACATAAAAA

SEQ ID NO:1156 CCTCACGAAGTATAAACAAAACAAAACCC

SEQ ID NO:1157 CAAGCACTGCCAAGAAAACAAATG

SEQ ID NO:1158 TGGAAGCTTGTCAAACTTACTGATCAG

SEQ ID NO:1159 CAAGGTCACTGAAGCTTCCCTT

SEQ ID NO:1160 CAAAGTCATTGCCTGGCACAAAT

SEQ ID NO:1161 GCGACTTCGCCGAGATGT

SEQ ID NO:1162 GGTTCTTTCGGTCACTGAGGAA

SEQ ID NO:1163 CCAAGCATTTCATTACCAGAGTTTACATTT

SEQ ID NO:1164 CAGTCAGTTTCTAAGTTCTCCACACA

SEQ ID NO:1165 CTCAGGAGGGTCTCGTCTGT

SEQ ID NO:1166 TGTTCGGTTCACGCCATGATAG

SEQ ID NO:1167 GCTGGATTATAACTCCTCTTCTTTCTCTG

SEQ ID NO:1168 GGCTGCCATCCCTGTAAAAGAG

SEQ ID NO:1169 GTGACTGCTCCCTCAACTTCAA

SEQ ID NO:1170 CAGATACAAGGAGCCACATCTTCTC

SEQ ID NO:1171 CCGTCCATGATCCCATCTTGTC

SEQ ID NO:1172 AAGAAGGAGCAGGTTCTGGAAC

SEQ ID NO:1173 GCCTGGCCTACGTATATATTTTTAATCACA

SEQ ID NO:1174 GAGCGGTACTACAAGATGAAGGAAG

SEQ ID NO:1175 TGAAGTAAAAGGTGCACTGTAATAATCCA

SEQ ID NO:1176 TATAACAAAGGACACGCAGGAGTCACT

SEQ ID NO:1177 TACAAGCAGTCACAGCACATGA

SEQ ID NO:1178 GGTAGTAGGAGTTGTACTGGTGGTT

SEQ ID NO:1179 GGACGATATTGAACAATGGTTCACTGA

SEQ ID NO:1180 CAGGTGGACAGCCTCTTTCAG

SEQ ID NO:1181 GCAGGTAGCTCAGACCATTCTC

SEQ ID NO:1182 CCCTTTATTGCTATTATGCAGAGCCAA

SEQ ID NO:1183 TCCCTAGAATCTCCTCACACGTATG

SEQ ID NO:1184 TTTTGTAATCGACAGGGCATGCTA

SEQ ID NO:1185 GTGTTCTGTGATGGGCCTTTTC

SEQ ID NO:1186 GTGCCACCTCACTTTGTTGAAT

SEQ ID NO:1187 GCTCTTTTGCCTGTTCTCTGCTT

SEQ ID NO:1188 AAGCAATGAGGCTGTAGCTCAA

SEQ ID NO:1189 GATTCTGAGGAACTGATCATGAGGT

SEQ ID NO:1190 CTCTGGAAATATGTGGGTTCATGGA

SEQ ID NO:1191 CACTTGTGTTCTGGTGTGGAATATCT

SEQ ID NO:1192 TGCTGACTGAAGATTCTGCCTTT

SEQ ID NO:1193 GGTGAGCAGCTGTTGAACTGAA

SEQ ID NO:1194 GAGGAGAGACTTTTGAGAAAGCCT

SEQ ID NO:1195 TAAGCTCAGGTCCTGCGTAGAT

SEQ ID NO:1196 AGGAGACTACCCTTCTTGCTCA

SEQ ID NO:1197 GGTTATCCCTTGCTGGAAAGCA

SEQ ID NO:1198 CCACTCCAAATAGTCACACCACA

SEQ ID NO:1199 CAGGAGTATTCATCATGATTGCATCTTCT

SEQ ID NO:1200 GAGTACTGGCATCTTTAGCCAAAC

SEQ ID NO:1201 ACAACGTCAGCTATCCAGAGAAAAA

SEQ ID NO:1202 CCATGACACCTCCTACATCCTCA

SEQ ID NO:1203 TGCATACTACTAGATGACCACAAGGAATA

SEQ ID NO:1204 GTCTTGAGGCTGAGGATCATGTG

SEQ ID NO:1205 CAAGGGTGGTTGGGAGTAGATG

SEQ ID NO:1206 GCGGATCCTGGAAAAGCTCTG

SEQ ID NO:1207 CATGTCAACTCATTACATTTAGTCAGCAAC

SEQ ID NO:1208 CCTTGTCAGCAACAGCCTTTGTA

SEQ ID NO:1209 GCCCAGCCCATGTAATTTTGAC

SEQ ID NO:1210 TGGATAGAGTTGGCAGCAACATG

SEQ ID NO:1211 AGATTGTGAAAATAGCTTTGGGTTTTACAC

SEQ ID NO:1212 TCTTTGTTCAGGGAGAGATGGTTTTT

SEQ ID NO:1213 GCTTGGGCAAAGGAAATATAATACTGAGAA

SEQ ID NO:1214 GGAGCAGGTCAACCTCATGTTC

SEQ ID NO:1215 GGGTAGAAGCTGAGATAGTATCATCAGTA

SEQ ID NO:1216 AGCACAGTCGAAGAAGACAATGA

SEQ ID NO:1217 CCTGCATGCTCTACATCATGTACC

SEQ ID NO:1218 CTCCTGCTCCAAGGCTGATTAT

SEQ ID NO:1219 TCCATCTTTCTCTAGAACTGAGTTTACAGT

SEQ ID NO:1220 CGTCCTGGACGATGCAGAAG

SEQ ID NO:1221 GTGCTGAATCAAACAAAAATAAAACCTGC

SEQ ID NO:1222 CCTCTTACCTGGGTCACACATTTG

SEQ ID NO:1223 CACCGTATATACTCAACAAGTGTTAGCT

SEQ ID NO:1224 TTCAAATGTTTCATCACATTTTCAAACGG

SEQ ID NO:1225 TCACTATTGGTAACAGAAAAGCTGCA

SEQ ID NO:1226 CGCGTTGTCAAGGACAAGATC

SEQ ID NO:1227 CATTGAAGGTGTCAACCAATAAACTTCTT

SEQ ID NO:1228 GAGACTTGATCAAGTCCAACATCTACC

SEQ ID NO:1229 GCACATCTAATCTAAGAGCCCTTCTTTTT

SEQ ID NO:1230 CCAGGACTAGGGTCGAATGAAG

SEQ ID NO:1231 CCTGAGCATTGTCAGTGTTGGA

SEQ ID NO:1232 GATCTACATGAGGAACTGTAGATGATGTAT

SEQ ID NO:1233 GGGAATAGAAAACTTGAGGGAATTGCT

SEQ ID NO:1234 GGAGGCATCCTACCCTTTTCTG

SEQ ID NO:1235 ACTCTGATCCACTCAGGTTTTGG

SEQ ID NO:1236 CAAAAAGAAATGATGTTGGAGAAGGGAA

SEQ ID NO:1237 CAGAGGCCCTTTCTTCAAGGTA

SEQ ID NO:1238 AAAGAAAAGCTCCTGAGGTGTAGAC

SEQ ID NO:1239 TTCGCATAGCTGAATCAGTAAGGAAG

SEQ ID NO:1240 GCCAACTTTTGCATGTTTTGTAGAGATG

SEQ ID NO:1241 CCTGAGGAGCCATGCCTATG

SEQ ID NO:1242 GAATAAAGGCGCACCGAGTAGA

SEQ ID NO:1243 ACCTGAAGAGTCACCCATGTCT

SEQ ID NO:1244 TGTGTGGAATTTGTTACTGAAGAAGGAAA

SEQ ID NO:1245 GGAGCATCGTGTTGTTGTGTC

SEQ ID NO:1246 GCATTTGACATCATCACTGTTACCTAAAAT

SEQ ID NO:1247 CATGGGATCAACAGCCATTTTGG

SEQ ID NO:1248 AAACAACTCTTGTCTTTTCACTCAAATAGC

SEQ ID NO:1249 CTCTGTCTGCTGAAGCTTTTTGC

SEQ ID NO:1250 CTTCAAACACCCATGCTCTTAACAC

SEQ ID NO:1251 CCTATCACAGATTGGTTTCCCTGA

SEQ ID NO:1252 GTCCGCTTGTGTCTCCAGTT

SEQ ID NO:1253 AGCAATGGACTTCACCTCATCAAA

SEQ ID NO:1254 CGGGCACGATTTTGCATTGAAT

SEQ ID NO:1255 GATCAGAAGAAATCACTGATGTGGATACA

SEQ ID NO:1256 GATGGCTGGCCTCTCTCTATTT

SEQ ID NO:1257 TAGAACAAACTTATGCAACAGTTAAGTCCT

SEQ ID NO:1258 GAGCTGTGGTGGTTGAGACT

SEQ ID NO:1259 AAAACAGTTGTTGTTTAGAATGAGGAGAGA

SEQ ID NO:1260 TTCAAGCCTTTCTGCTGGAGAA

SEQ ID NO:1261 TAAGTGGAGAGAGCCTGATAAAACAAAC

SEQ ID NO:1262 GCAGTCTGCCTCTTCACTCTTTAATTTTAT

SEQ ID NO:1263 CCTTCCTAACAGTTTACCAAAGTTGAATCA

SEQ ID NO:1264 TTCTGTCCTGGCTTTAGTCCTTG

SEQ ID NO:1265 TGCCACTCAGAAAATCTAGCTTTAACT

SEQ ID NO:1266 CGCTCTTTGTTGCTTCCCAAAC

SEQ ID NO:1267 TGAATACTACAGGCAACAGAAAACATACA

SEQ ID NO:1268 CTTATGGCAGCAGCCAAGAATC

SEQ ID NO:1269 ACTGAGTCTAAAACATGGTCTTGCA

SEQ ID NO:1270 CAGAGAATCAGCCCTCCAGAAAC

SEQ ID NO:1271 ACCTGGCCTTAATTTCCACATTTGTAA

SEQ ID NO:1272 CAGCCACCAAACTAAACAAAGACTTTTAAT

SEQ ID NO:1273 ACCGATTTTAGATGGCATCTTAATAACACA

SEQ ID NO:1274 GTCACCTCCCAGACCAAAGATT

SEQ ID NO:1275 TGCCCAGAATACTTGTGCTTCTT

SEQ ID NO:1276 CCCAGACGTACGTGACAAAC

SEQ ID NO:1277 CGTAGAAGAGGACCTACTAATCCAGTG

SEQ ID NO:1278 GCAATCGTTCCATGAAGACAGGT

SEQ ID NO:1279 AGGTAAGACTCAGGGCTGGAAT

SEQ ID NO:1280 ACTTAACACAAGAGGTCAAAGCCA

SEQ ID NO:1281 CTGTGCTCTATGCCAACATTAATTTTCC

SEQ ID NO:1282 GAACTTGATGGCAGTGGCTTTG

SEQ ID NO:1283 TATTGGAGCCTCCGCACCAACA

SEQ ID NO:1284 CCCAGAGTATCAACAGCTTCTTCAG

SEQ ID NO:1285 CTGACAAGGTACCGCGAGACAT

SEQ ID NO:1286 AGCTTCTGTGTATTTTAGTCCAAGGAATG

SEQ ID NO:1287 GGAGCTGGCACTAATGATACAGG

SEQ ID NO:1288 GGAGGAGGATTTTGTCATCCTCAAC

SEQ ID NO:1289 GTAAGTTGACACCCTGGAGCTAAG

SEQ ID NO:1290 GCCTGACCTAAATACTCAGCTTCA

SEQ ID NO:1291 GGGAAAGGCAGGAGAAATGAAGTA

SEQ ID NO:1292 CCATCCAAACCAGGAAAAATCATTTTTCAT

SEQ ID NO:1293 CCATGGAGTGCCTGTTTTGTGTA

SEQ ID NO:1294 TGAGCATTCTGCTATTAGCGAACT

SEQ ID NO:1295 CATCCATGGCTATGGTGTCCAA

SEQ ID NO:1296 AACTCAGAGGGCTTTCCAGAAG

SEQ ID NO:1297 CTCCCTGCTGGAAAAGTTTGAG

SEQ ID NO:1298 GGGCCATCTACTGGGAGTAGTT

SEQ ID NO:1299 GGACAAGAAGGATATCTTCAATGAGCA

SEQ ID NO:1300 GCATAGATGCTGCTGAAGGTGA

SEQ ID NO:1301 GGGAACAGGAGGCAAAATAAATCTTT

SEQ ID NO:1302 CCATGCTGACTCAAGATTTGATAGTTAGA

SEQ ID NO:1303 ACTCACTCAGTTAACTGGTGAACATAAAAT

SEQ ID NO:1304 GGATGTAATATTTTCTGAAGAGCCAAACAA

SEQ ID NO:1305 GCCAATTGGTACATAAGAGGCAAAAATTT

SEQ ID NO:1306 GGAGCATATGATTTTATGGTAAAGGTGTG

SEQ ID NO:1307 CAAGTGCTCTGTAATTGATTACAAAGGTT

SEQ ID NO:1308 CCACTTTTTATACAGTTCTACCCTGATCAA

SEQ ID NO:1309 GGCCTCCCAAAGCATTATGAATATG

SEQ ID NO:1310 GGATATCAAAACAGGACAGTGTTTACAAAC

SEQ ID NO:1311 GGTCAATATCACTAAGGACAAAAACACAAA

SEQ ID NO:1312 TCAGTGGTTTTGAGCAGAGAGATG

SEQ ID NO:1313 ATGACCCACAGCAAACAGAACTG

SEQ ID NO:1314 CTGTAAAACTATGGGACTTGAAAACGG

SEQ ID NO:1315 GGATCTCGAATCAGGCGCTTAA

SEQ ID NO:1316 CTCCTCAGGAAACCAAGAAGAACAAGAG

SEQ ID NO:1317 TTATAGGCTTTTTGTGAGAACACAGGT

SEQ ID NO:1318 CTTGGGTTCTCAGCCTTTTAAAAACTATT

SEQ ID NO:1319 CCTGAAGGAAAGTTGTATGAAGTAACGT

SEQ ID NO:1320 CTAACTGCCCTGGCACATCT

SEQ ID NO:1321 CCACAGACATGTAAAGTATGTGAGAAGTAA

SEQ ID NO:1322 GCGACAAGGTAACCTGCTGT

SEQ ID NO:1323 GAGTTGCTTCAAACTGATGGCAAATATAA

SEQ ID NO:1324 CGGGAGAGGCACTGTAGGT

SEQ ID NO:1325 CCGTTTTTACCCTGTAAGTGAGGA

SEQ ID NO:1326 GGTGAATACTCAGAGGTTTGCTGA

SEQ ID NO:1327 TCATCTGATTTCATCCTACTGACTACTGT

SEQ ID NO:1328 AGATCCTCAAACACTGCCTGAATG

SEQ ID NO:1329 CACTCTCATCAAATCCGACATCGT

SEQ ID NO:1330 CCAATCTAATGGATGAAAATGGTATGCTTT

SEQ ID NO:1331 GTTGAGTCCCATAGGGAAGGTAGT

SEQ ID NO:1332 GCCATCACAGATATCCAGGTGT

SEQ ID NO:1333 GCCTGGGTCAGACAGAAGAGAT

SEQ ID NO:1334 GCCACTCGTACTTGCCCTCTA

SEQ ID NO:1335 AGTGGGTTCTAGGTGTCCTTGT

SEQ ID NO:1336 GTAATGTCTTTTTGCATAGCTCTGCAT

SEQ ID NO:1337 CATGTTCCCTGCTTACTTGCAG

SEQ ID NO:1338 CTCTGTAAAAATCAAGAAGCAATTGCTCA

SEQ ID NO:1339 CTTGCAGATACCTTGTTTAGCAAGG

SEQ ID NO:1340 CTGTGTGTTTTGAGTTGACTCTTTTTAAGA

SEQ ID NO:1341 TTCGAGCACAGCTCCAAGGAGT

SEQ ID NO:1342 GAGCCAGTACAATGAAAAACTTCATTCA

SEQ ID NO:1343 GCAGAGTCTGATGTCACACAGT

SEQ ID NO:1344 AGCTTTCTGCTTAGGAAACACATTTAAAAA

SEQ ID NO:1345 CTGATTCAAGGCTTTTACAGGAAAGG

SEQ ID NO:1346 GGGAAGATAACATATGTGCTCATCTAACT

SEQ ID NO:1347 TTAGGTCCGGAAACAGCAGAAGGA

SEQ ID NO:1348 CCGAAATATAAACATTTGGGCTAAGCTTTT

SEQ ID NO:1349 GAGTTGTCCTCATCTTTTCGCTTTTT

SEQ ID NO:1350 GACCTGGAGACCAAGAAGTTCC

SEQ ID NO:1351 AATGAACAATTTCTCCTTTCCTCTCCTT

SEQ ID NO:1352 CGCAACAGCTCCTTCCACTT

SEQ ID NO:1353 AGTCAGTGAGATGCCCAGTACA

SEQ ID NO:1354 ACTAAAGGACTCTGCGAGTCTGA

SEQ ID NO:1355 GAACTTGGCTGTAGTGACCCTT

SEQ ID NO:1356 CCGACACCGTCCTGTCTTC

SEQ ID NO:1357 TTAAATCTGGAGAACTGGGTAAAGACATG

SEQ ID NO:1358 CGGCATCAACTCGTTCACCT

SEQ ID NO:1359 GAATGACCTCATGGATGGAGATACAT

SEQ ID NO:1360 GCCAGGAGTTTAAGCTGGATTC

SEQ ID NO:1361 CAACAGTAAATTTGCTAAGAGCCGAA

SEQ ID NO:1362 CAACGTGGACGACTGCAATC

SEQ ID NO:1363 TCTCTTACCTAAACTCTTCATAATGCTTGC

SEQ ID NO:1364 GATGAGAAGTCGATTCTGGCTTCA

SEQ ID NO:1365 ACAATCTTGATAGAGGGAAACTGTCTAGA

SEQ ID NO:1366 CAACGCCTACCTCTGCTTCT

SEQ ID NO:1367 CAGGAGGATGCATGTCATAAATATGATTCT

SEQ ID NO:1368 CCAGACCAACATCAACGAGTGT

SEQ ID NO:1369 CACTCGTATAGCATACTTTGCAGATACTTT

SEQ ID NO:1370 GCAGAGGAAGGAGTGATTTAGGC

SEQ ID NO:1371 TGAAACAAACAAGCACATTACAATATAGGG

SEQ ID NO:1372 TGAGGAGGACATCAACGAGTGT

SEQ ID NO:1373 ATTGGGATTACCCTTTTGCAGGT

SEQ ID NO:1374 CCTTCAATTTTTCCTAAGGATGCTTCTTAG

SEQ ID NO:1375 AGAAGTTCTACCTCTTTTCCACCTCA

SEQ ID NO:1376 TGGTGTAAGGCTAAAGGGTATTTTTATGTT

SEQ ID NO:1377 GGCTTTTGGCCTTCATCTTAACC

SEQ ID NO:1378 CTTCCTTTTAACCAGCCACTCCAT

SEQ ID NO:1379 GGCCAATGGAGATTTCAAGTAAGACA

SEQ ID NO:1380 AAGCAGTCAACTAGATACTGTTTGCT

SEQ ID NO:1381 CCTAACTGTGTCTGCTACAGCA

SEQ ID NO:1382 GCTAGGTGGATATTACAATTTAAGTGGCT

SEQ ID NO:1383 GCTTCCATCCAGGGATCACATA

SEQ ID NO:1384 GGCATTATGCAGTGTAAAGAAGATTAAGC

SEQ ID NO:1385 AAACGCAAGCGTAAACCATATCG

SEQ ID NO:1386 CGAACAGAAAATGTGGATCATATTCATGT

SEQ ID NO:1387 TGTGACAGCCCATACAGATGTC

SEQ ID NO:1388 CTTCAGATAAAGGATTGTAAACCCATGTTT

SEQ ID NO:1389 CCCATGCTGAAAGATGTCATGCT

SEQ ID NO:1390 GTATAAAACAATGGCATGCCCTTTATGT

SEQ ID NO:1391 CATGAAATTTCCAGGGCTTAGCATG

SEQ ID NO:1392 CAATAATGGCCACTCATTATCATAATGACC

SEQ ID NO:1393 TGTAGTAGTCACATTGTACTAGCCACA

SEQ ID NO:1394 ACTCCACATCAGTGGAAATTTAATGTGA

SEQ ID NO:1395 AGTGCATTGTAAAAATGTATGTCTTGATGG

SEQ ID NO:1396 CAGCTGCATTTAGAAATCAATAGCATACAT

SEQ ID NO:1397 GGTTGTGCATCAGTAAGCTATTTTATTTGT

SEQ ID NO:1398 TTACAGATGCAGCAGCAGAACCT

SEQ ID NO:1399 AGCAATAGATCCTTTACTGAAGAATGCAT

SEQ ID NO:1400 CATGCATCACGACATCGTGA

SEQ ID NO:1401 AGTGCCAATATTCAGAGGCATAAGTTTAA

SEQ ID NO:1402 CCTTTGTGCTTCTGTTCTTCGT

SEQ ID NO:1403 GCATTGCCTTATTTCAGGTTATAACTTCTT

SEQ ID NO:1404 CGTCTACCTGGAGATTGACAACC

SEQ ID NO:1405 CGCTTACACCAAAATCGATTTCTATCATTT

SEQ ID NO:1406 GGCAACCGAATGAGGAAGATCTC

SEQ ID NO:1407 CCACACAACACATTTATCTACAAATGCTTT

SEQ ID NO:1408 CAGCTTAAGAGCAGTGTAATTAGTAGGTTC

SEQ ID NO:1409 TGTGTATCTACAGTTTGGGAAAAACTTTGA

SEQ ID NO:1410 TCAAACAACTTTAAATGGGTCACCATTTT

SEQ ID NO:1411 CTGCCTCTTCTTAAGAGATAACACATGAT

SEQ ID NO:1412 CCTCTAGGTGGCAGTTCTGTCA

SEQ ID NO:1413 GGACTTAGTCTGTTGCCCTCAA

SEQ ID NO:1414 TGGAAGTGATTGCGCTAATGGTA

SEQ ID NO:1415 GCAGTGTGAGAACAGACTCAACAG

SEQ ID NO:1416 GCTTAGACATCACACTGTCAATAGATCTTT

SEQ ID NO:1417 ACATTTGGTCTAATGGTACAACTGCT

SEQ ID NO:1418 TTCTCGAACTGCTGTTGTCATGA

SEQ ID NO:1419 CCATGTTTTGGCTCATTCATGCT

SEQ ID NO:1420 CGAGACACACTGGTATTAAGATTGTACAA

SEQ ID NO:1421 CCTCTGTGGTATAATGCAACTTGTTTTT

SEQ ID NO:1422 TTTGAAATGTCCTCTATGATGGTTTGGA

SEQ ID NO:1423 CCATGTTAGTCTGTGAGATATTGCTAACTT

SEQ ID NO:1424 GGTCACAGATTAAAGATGTGAAAGGACAAT

SEQ ID NO:1425 CAAATCACTACTATTGGCAACTTAGTGC

SEQ ID NO:1426 ACCAATCATATAAACTTTCTGGGAGAACAA

SEQ ID NO:1427 TGATGATAGAAATCTGATGGTCCAGACA

SEQ ID NO:1428 CCGATGAAAATCACATTGAATTTAGCAACA

SEQ ID NO:1429 AAAAGCTAGTGCACATTGGTTTAGAAAAT

SEQ ID NO:1430 TACAAAAGGATCCATGGCAGAGAAAA

SEQ ID NO:1431 GGGTTGTTCCCAGTTCTGAATTAATTTATC

SEQ ID NO:1432 AAGATTGGCAGTAGAAAAACCTCTATCAAT

SEQ ID NO:1433 TTCTGATAGGTTTATTTGGGCTTTTCTTCT

SEQ ID NO:1434 TCCGATATTCCACATCAGCCAATG

SEQ ID NO:1435 CAGGTTGATGTTTATGTATCCAGTGTGA

SEQ ID NO:1436 GAGTTACGAAAGCAAAACCAAACAAAATT

SEQ ID NO:1437 TGAAAATGATTGTTCTTGTGGTCCGTAT

SEQ ID NO:1438 CCATTGTTCACATCTGCAGCAAAAA

SEQ ID NO:1439 AATCTCTTGAATTTGGAGCATCTGACT

SEQ ID NO:1440 GCTACCAGATCAGCACTGCATT

SEQ ID NO:1441 TGAGCAGCATAGTTGAAGATCATTTATGTT

SEQ ID NO:1442 CATCACCATGTCAGAAGATGACCA

SEQ ID NO:1443 CCACGTCCCTTAGTAACCAGGT

SEQ ID NO:1444 ACCTCATCCCACTGATATGTAGAATCTAAA

SEQ ID NO:1445 CCTTTGTGCTTAACACAATCCTTGAA

SEQ ID NO:1446 CGGCCTCTGTTCCATTTCTTGT

SEQ ID NO:1447 ATGCTTTGATGCCTATATCAGGAACTT

SEQ ID NO:1448 AAAGTGGTACTTGGCACAATCCA

SEQ ID NO:1449 GCAGTCTAGGAAAAATTGAAAGGTGT

SEQ ID NO:1450 GTCTGCCACTATCCCTTTTCCT

SEQ ID NO:1451 AAGTCAACCACTGTAGCAACTACTG

SEQ ID NO:1452 TTGACTTTGCACTATAATTAGGAATGGCT

SEQ ID NO:1453 CAAGTTGTTCCAGGATCAGTTCCA

SEQ ID NO:1454 CGCTCCGAACCTTGTCCT

SEQ ID NO:1455 CAAGCTGGTATTCAGAAGCCTTAAATAAAA

SEQ ID NO:1456 ACACTCTCACTTCCTGGACCTA

SEQ ID NO:1457 GCTGTGGCCATTTTCCATACTAGTAAAT

SEQ ID NO:1458 ACAGTCTTGTGATTAGAGACATTGAGTG

SEQ ID NO:1459 GGTAAAGATGGTTAAATGATGATGGAAGTG

SEQ ID NO:1460 GATGGTCTTCAACTGTCATTTGGTG

SEQ ID NO:1461 GCCTAATTCAAAGAGGAGAATCAATAGCAT

SEQ ID NO:1462 GAACTAATGAATGGAGTGAAGGAGGAA

SEQ ID NO:1463 GATGGATGTATTTCAGCATCTTTGAAATGT

SEQ ID NO:1464 CGGTTCTCTGGGTCCAATAAATACC

SEQ ID NO:1465 GAAATAAGGAATACAAATGTGCTGCAGAAT

SEQ ID NO:1466 CGTAGCTGCAGGTGTGAGTG

SEQ ID NO:1467 TGGGACAGTTTCTGAATCTTTCCTTTATTT

SEQ ID NO:1468 ACCCTAAGTCCGCGATCCC

SEQ ID NO:1469 CCTAGAAACAGCTCCTGGCAAA

SEQ ID NO:1470 ACGTGAGAATTGGAACTCCCTTTTC

SEQ ID NO:1471 CACCTGAAATCACTAGGCATGTGT

SEQ ID NO:1472 ACCACATTCAGCCTGAATTATTTTCAGTA

SEQ ID NO:1473 TGTAATATTTTCTCAGCACATATGTGTCCA

SEQ ID NO:1474 TGATGTTGCTTTATTTCCTTGGAAAAGC

SEQ ID NO:1475 GTCTACTAAGAAAGTTAAAAAGCATTGGCA

SEQ ID NO:1476 CTGGCATGCCTAGCTACTTTTTCTA

SEQ ID NO:1477 TGAGGATATGACTCAATTCAGGTAAAGAGA

SEQ ID NO:1478 AGTTCACCATGTGTGTGTTCCA

SEQ ID NO:1479 GCTGACAGATTAAGCGAAGACTTACATATT

SEQ ID NO:1480 CCTGGCTGTAAAAATTGCTAAGTACAGTAA

SEQ ID NO:1481 GCTAAAGATGGAAGTATTTCCAAGAATGAC

SEQ ID NO:1482 GGGAGGAAATAAGATGTACAACTGCA

SEQ ID NO:1483 CATATTAGCAATCTCAAGGTCATGCTTTAA

SEQ ID NO:1484 CCCACGATCCTGATTTAGGTCAG

SEQ ID NO:1485 CGATGTCCAGGATCCAGTCAAC

SEQ ID NO:1486 ATGGAAAACTGGTCTATGCTGTTTCT

SEQ ID NO:1487 CAGATATCTAAAACGCAGAATCAAAACACA

SEQ ID NO:1488 GTGACTTCTCCACAGTTCAAAGC

SEQ ID NO:1489 ATTCTGATGAAAAGCACCTAGAGAAACT

SEQ ID NO:1490 CTGGGACATCACCCTCACTTAC

SEQ ID NO:1491 GGTCAGAAAGACTTCTACATATCACAACAA

SEQ ID NO:1492 GCAAACTCCATCTCTAGCGTTCA

SEQ ID NO:1493 AGCTCATATTAGAGTCACTTAACCCTCA

SEQ ID NO:1494 CTGAAGGTGATGGCATAGGAGT

SEQ ID NO:1495 AGAGATTTCTCATTTTCTTCTCAAGGCAA

SEQ ID NO:1496 AGCTTCTCAGAGTCGGAAGACT

SEQ ID NO:1497 GACTATTAGCACCAGCAATAACCCAT

SEQ ID NO:1498 GGGTGTAGCAGTCCTCTAGTG

SEQ ID NO:1499 GTAATGAACAGACTAATGTCTGTGGTAGAA

SEQ ID NO:1500 ACACCTGACCCATTCCTCAAAC

SEQ ID NO:1501 CGTGTCTAGTCTCCAAAGTGCA

SEQ ID NO:1502 ACAAGATAAAGAGGCTTTATTGAACTCACT

SEQ ID NO:1503 CTGGCTGGACCTATAAAAGGCA

SEQ ID NO:1504 GCACCATGTCCCTGCAGTA

SEQ ID NO:1505 CCTTGGCTTTTGACTATAATCAAAGAAGAA

SEQ ID NO:1506 GGGTGGATGTCATTGTGCAGAT

SEQ ID NO:1507 TCACTCATGTGATGTCATCTCTCCT

SEQ ID NO:1508 AACAGGAGCATTGATATACGTCATTGA

SEQ ID NO:1509 CCATATCTGTGAAATGCTGGCATTT

SEQ ID NO:1510 AGCGTGTTGCCTATCTACTTTAAGTG

SEQ ID NO:1511 GTTGGAAGTGTCTCATGCTGGAT

SEQ ID NO:1512 GCCAAAATGCCAGATTGCAAATTCT

SEQ ID NO:1513 CAACCCACTTGGCCTGTCTT

SEQ ID NO:1514 GCCTCTTCTCTAACCAGAGAAATAACTAT

SEQ ID NO:1515 ACTTACCTAGGACGGTGACCTT

SEQ ID NO:1516 TGCCGACACCAGAATTTAAGATAATAGAAA

SEQ ID NO:1517 ACTCTTACTCTCTCCTTTACGAGCT

SEQ ID NO:1518 AACCATAAAGAATGAAAACTTGCAAAGTGA

SEQ ID NO:1519 ATAACACAGATTCTAACCCAGCTCTTTTT

SEQ ID NO:1520 GAGCAAAGACCGGAGACGTATT

SEQ ID NO:1521 GCTGCCAATACTCCTTGGACT

SEQ ID NO:1522 GCAGCCGGTAACCAATCTCTAA

SEQ ID NO:1523 TCGAAGATTGTTGATCATGTATGTTCAAC

SEQ ID NO:1524 CTGTGCCAGTACGATGTGGA

SEQ ID NO:1525 AAAGGTCAGTTCTGATTTTTCACAATGTG

SEQ ID NO:1526 GTGATTTCTTTTCAGTTTTCTGTTGCCT

SEQ ID NO:1527 GCTTGCCAACTTCATAAGAGAAAAGAATTT

SEQ ID NO:1528 CCTAGAGACTATACAGAGCTACAACAGTAA

SEQ ID NO:1529 ATGGTTCAGATTTCACTGCCTCAT

SEQ ID NO:1530 GACTACTGTGGCCGAAAGTTTG

SEQ ID NO:1531 ATTTGCCATTGCACAAAAGTATTGACT

SEQ ID NO:1532 CCAGTAGGACATAGAACTTGGTGAAC

SEQ ID NO:1533 GGACTAGTTGAATGAGTTTTGACAAACTAC

SEQ ID NO:1534 CATGTGATTGCAATCCCACCTATTC

SEQ ID NO:1535 CTGGATTCCCTCAAAACCTTCACT

SEQ ID NO:1536 ACCACACTCAGTTCCAAACCC

SEQ ID NO:1537 TGCATGCCAGAAAAAGATCAACATAAAAT

SEQ ID NO:1538 ATCACCAAGGCCATCGAGAGCT

SEQ ID NO:1539 GCTGTCGCTTCTGAATGTATGTC

SEQ ID NO:1540 AAACAGGAAGAACAGGATAGAAAGACTG

SEQ ID NO:1541 ATGGGTAGTCATTGTCTTTTATCTTCCC

SEQ ID NO:1542 CGTTTCTGTCCCAAGTCCACA

SEQ ID NO:1543 GATTAGACCAGTTGACGAAAGTAGTGA

SEQ ID NO:1544 ATCTGCGAGCAGACGGAGAG

SEQ ID NO:1545 GATCATCTTGATGAAGTATGGCTTCAGAA

SEQ ID NO:1546 CACTCAGCTGTGTACCTGAGG

SEQ ID NO:1547 CGCTCGGAGCGTGTTTTTATAA

SEQ ID NO:1548 GGAGTGCCAATGGACGGATG

SEQ ID NO:1549 GCTTTCTTCCACTGTTGTCTGAAC

SEQ ID NO:1550 AAATCTCGGACCGTCCCTTC

SEQ ID NO:1551 TTTGTCATCACGCTTCCCTTCT

SEQ ID NO:1552 GCATTCCAACGTCTCCGACTG

SEQ ID NO:1553 GATCGGACATCGAGTTCTGTAGAG

SEQ ID NO:1554 GGGAACCTATGAACATTCTGTAGGG

SEQ ID NO:1555 GTGTATGCAGCAAGTCGGGATA

SEQ ID NO:1556 AACGATAGTCCACCACGATTCAC

SEQ ID NO:1557 AGAACCTTTCTATACCTTTACAGTGTCAGA

SEQ ID NO:1558 CCTGGTGATGGAGGTGAAAACTA

SEQ ID NO:1559 AGATGTTTGGGAATCACAGCAAGA

SEQ ID NO:1560 CCTCAAGGAACATCATGAACACTTTCA

SEQ ID NO:1561 CTGTCCAAAACACAACTCAGTTAAGAAG

SEQ ID NO:1562 TGGCTGGTTTGTCCACTAATACAAC

SEQ ID NO:1563 GTTGAAGGTTCCATTCAAATATTGACATCC

SEQ ID NO:1564 TCCTTGGAAGCTCTTTGCTAGTATATTAGA

SEQ ID NO:1565 GCAACCGGCTCTCTCTATACTTC

SEQ ID NO:1566 CCCTCGATATGTCTGAACCTCAA

SEQ ID NO:1567 ACACACTGACATCTGTTTTCTTTTCCA

SEQ ID NO:1568 TAGTTGAAGTGAAAGTTCTGGATGCAA

SEQ ID NO:1569 GCCTTTGAAGCCTTTGATGGCTA

SEQ ID NO:1570 AAGTCGAAGTCCTTGCTACAATTACTC

SEQ ID NO:1571 CCTCATACAGCGTTTCTTTACCTGAA

SEQ ID NO:1572 CGATTCTGATTTCTTCTGTTTCAGATTCC

SEQ ID NO:1573 GAAACAATCTGGACCGAAATTCCTTC

SEQ ID NO:1574 TCTCTCCTCATTCCTGACTTCCTT

SEQ ID NO:1575 GAGAGTAATGTGAACAGTCATTGCTTTTAT

SEQ ID NO:1576 GCTAGACATAGGGCAATACCTTCTC

SEQ ID NO:1577 TGTGCTGCTTTACGCTTTTCTTTTT

SEQ ID NO:1578 ACCTGGCCAAGAGTGTGATTTTAA

SEQ ID NO:1579 CCCATATAGATTTGGCTGTGTTCCA

SEQ ID NO:1580 GAGCTTTTACAGACCTCGGAGAAT

SEQ ID NO:1581 AAACAACAACAAAAAGAAACAGCTAAGCTA

SEQ ID NO:1582 ATGAGCATGGCAAATTTTTCATCGAA

SEQ ID NO:1583 TTTAGCATGTGCAATGAATTCTCACAG

SEQ ID NO:1584 TCCCAATAAATTTTGTGAACTTTTGGCTTT

SEQ ID NO:1585 AACCTGGTAAACGAGCAAAACTTTG

SEQ ID NO:1586 TATCCTTACTCCTGCCTTTATCCATTACAT

SEQ ID NO:1587 ATTCTGCTCATTATTTGCATTTGAAACCA

SEQ ID NO:1588 ATTCCTTCCTTCTCTAAAAAGGAAATTGC

SEQ ID NO:1589 GCCAATACTGGTGACAGGATTAGAGTATAT

SEQ ID NO:1590 CCCTCCTGTCTAGCACAACAAA

SEQ ID NO:1591 CCATGATGGAGTCCAGGACAAA

SEQ ID NO:1592 ACCTTCTAGTTTATCTCATATTCCCAGTCA

SEQ ID NO:1593 GCGAACTGCCTGTTAAGATCATTT

SEQ ID NO:1594 AGAAGCAATCTATATGCAAGATGAGGATG

SEQ ID NO:1595 GTTTACAGATGTTTTTCTTGTTCTAAGGCT

SEQ ID NO:1596 GCTTAACAGAACACATCAGTTATTTTATAACTT

SEQ ID NO:1597 CACCACTAACGCCAGCTTTTTC

SEQ ID NO:1598 CCGACTCATTAACTTATCAATGAGGAACTT

SEQ ID NO:1599 CGTTAATGAAGAAGGTAGTGACTGACA

SEQ ID NO:1600 CTCACAGACAGGTGCTTATGACC

SEQ ID NO:1601 GGGTGGAGAACTGATTGCCATA

SEQ ID NO:1602 GCTGTCAGTCTGTAGTAACTTTCCATT

SEQ ID NO:1603 CACCTTTCCCTCTCCCTAAAGC

SEQ ID NO:1604 TTCTTTGGTACTGCATCCACAGT

SEQ ID NO:1605 CAGGGCTTCATCACACCCAT

SEQ ID NO:1606 GTCCCAAGATGTTGATTTTTACTGTGTTTT

SEQ ID NO:1607 TTTAAAATATCAGCCTGTGCTGCAAAG

SEQ ID NO:1608 CCAAATTCAACGGGCTCTTGT

SEQ ID NO:1609 AAAAAGTAGTTTGATAGATGTGTTATCCTTTCA

SEQ ID NO:1610 GTGCCACCTCAACGACGCAT

SEQ ID NO:1611 GGAAACTGTGTGAGCATTTCCCA

SEQ ID NO:1612 CATGCCGTCGAGGAAACCT

SEQ ID NO:1613 ACATAAACAACTGTCGGAGCTTCTAC

SEQ ID NO:1614 CTACAACTGCGTGTGTGTCAAC

SEQ ID NO:1615 CATACTGATAGACAGCTGGAGCAT

SEQ ID NO:1616 AAGTTGCAGGGAAGTCTTAAGAGAG

SEQ ID NO:1617 GATTGTGGCCTTCTTTGAGTTCG

SEQ ID NO:1618 GTAAGTTCTAGGAGCAGTGGAAGAAG

SEQ ID NO:1619 CTGACCCATCAATCAACCAACAAC

SEQ ID NO:1620 CAGCAGCTTTTTCAGAGACTATCTAGT

SEQ ID NO:1621 ACCTCCATGCTACAGAAAAATTGCTAATA

SEQ ID NO:1622 CAAGGATACATTCTGATTCTATAGCTCACA

SEQ ID NO:1623 ACCTGAAAGCTTTAAAACCCAATTGAAA

SEQ ID NO:1624 ATCCAGCGCGACAAACAATCCAG

SEQ ID NO:1625 CCTCTTCCTCTATGGGCAGGTAA

SEQ ID NO:1626 TCGGAGGAGCAGATATGTCAGAG

SEQ ID NO:1627 GATTGTGCACACAAACCAAAACTGTA

SEQ ID NO:1628 AGAGAGAAAACAATGGGAGATAAGCAG

SEQ ID NO:1629 GGATATTCTCTTATCCCAGTCACCACTATA

SEQ ID NO:1630 CCTGAGCCTTTGAAGCAGGAG

SEQ ID NO:1631 AGTAGTACTATAAATGGCACTGGTGAGA

SEQ ID NO:1632 AGAAATGTTCCAGATCTCACTAGCTCT

SEQ ID NO:1633 AAATCATCTGTTTTCACCCACTATTTGC

SEQ ID NO:1634 AAATGAATCTAATGCTGACATTGAGAGGT

SEQ ID NO:1635 AGCAATGAGGCGGATCACAA

SEQ ID NO:1636 CAGAGAACTGCTTCCTGCTGAT

SEQ ID NO:1637 CCACAGGCCTCTATAAAATACGTGG

SEQ ID NO:1638 GCATAGGCTTATTGACCCATTGGT

SEQ ID NO:1639 GCATTTAAATAGCAACATGTGGCTAATGG

SEQ ID NO:1640 GCCTAGAACTGAACTCTCTCACACT

SEQ ID NO:1641 CTCCAACAAAGTTAAGAGAATTGTTACGT

SEQ ID NO:1642 AGGCTCTTTTTCTTTTTAATCAGTTTGCTT

SEQ ID NO:1643 CTCATTCAGACTGGAATCACCAATAGTAA

SEQ ID NO:1644 CCCAATGGGCAAGAAGTCTAGAC

SEQ ID NO:1645 TGAGACTGGCATTTGGATATTGGG

SEQ ID NO:1646 GCAAAGGACTGGAGTCATGTG

SEQ ID NO:1647 GTCCTCCGTGGAAGGTCTTC

SEQ ID NO:1648 CGAGATCGACGTCAACGAGT

SEQ ID NO:1649 CGAGTAGACAACATGTCATCCATAGTG

SEQ ID NO:1650 AAGTTGTGGACAGGTTTTGAAAGATATTTG

SEQ ID NO:1651 GTCCAGGACAGGCAGGCAAATG

SEQ ID NO:1652 GTTGCTTTTGTACCGTCATAAAGTCA

SEQ ID NO:1653 AGATGTCCAACAGCCGTGATTC

SEQ ID NO:1654 GGAAGAGAATCTCCGCAAGAAAGG

SEQ ID NO:1655 CCAAACTGGAATGGAAATTGGTTCTG

SEQ ID NO:1656 TGTAAGTCAGACATAATGCATGCTGAA

SEQ ID NO:1657 GACCTGCCTTCTGCATTTTCATC

SEQ ID NO:1658 AGGACAACTGTTTATGCTGAAAACAAAAA

SEQ ID NO:1659 CTGTCCGTATCTGTTGCTGGTT

SEQ ID NO:1660 AGTTTATCTGGCTGTGTAAATATCCACC

SEQ ID NO:1661 TCTGGAACCTCCTTTTTCCACTTC

SEQ ID NO:1662 AGTCTTTATCTCTTCATCAATGCAAATCCT

SEQ ID NO:1663 CCTGAGCCATTCTCAAGATTTCCC

SEQ ID NO:1664 AGGAAAGTCAAGAGGTAAGATGACATAGT

SEQ ID NO:1665 GACGTGAGCAGTTGGTTGAG

SEQ ID NO:1666 TCTGACTTATATGCTTCCTCTTCAGCTA

SEQ ID NO:1667 GCTCCGTGAGGTTATTGTTCAGG

SEQ ID NO:1668 GGGCTGGTATGGTACTAGTCCT

SEQ ID NO:1669 TTCGTTTTTCTTCTAGTTCTTTCTGGATCT

SEQ ID NO:1670 GCATGACTTAGTTCAGGTCATTCACTTTTA

SEQ ID NO:1671 GCTTCTGCTTGATGTTTAGGCAGA

SEQ ID NO:1672 TTTTGGACTTACAAGAAGTCAGGTGTT

SEQ ID NO:1673 GGAGGTGGGTTCATACCCATG

SEQ ID NO:1674 TTTGAGAACTTCTGCTTTATTCTGTCCA

SEQ ID NO:1675 GGATTAGAGTTGGCATACTTGGCA

SEQ ID NO:1676 ATGGAGGTGAATGAGACCCAGTA

SEQ ID NO:1677 TGATTTGGGAGCATATGCTGCT

SEQ ID NO:1678 GAAGTCAGCTGCTATGAAGAAACAGA

SEQ ID NO:1679 CATCAACAGATTCCTATTAGGTGGGAA

SEQ ID NO:1680 GCCAGCCTCCTTATTACTCCTG

SEQ ID NO:1681 TGTCCCTATTGGCCTATAGAAAGTAATCA

SEQ ID NO:1682 GAAGATGAAGATGCAGAGGACTCTTC

SEQ ID NO:1683 GCAGTTGCTTGAAGTTCACCACTA

SEQ ID NO:1684 CCCATTTCTTCACCAAGTCCTGT

SEQ ID NO:1685 CTCCTAATAGCTCCTTGATGCTGT

SEQ ID NO:1686 GCTTCTCCATTTTCAAAGCCTCTT

SEQ ID NO:1687 GCACATGACAGATGCTCAGTAAATAGG

SEQ ID NO:1688 CTGATGCTATAACCATCAGATGCTCTT

SEQ ID NO:1689 CCCAGTAGGGATTTTTGTCTATTGTAAGA

SEQ ID NO:1690 GGGACAAGTGCCTATAATTTCTTTTATCCA

SEQ ID NO:1691 GGGAACCAACTGATTCTAATCTTTGAGG

SEQ ID NO:1692 GAGTGCTTTCTTTGGTGAAGTTTCATTT

SEQ ID NO:1693 GCTACCGTGGCTGTACTTGAAA

SEQ ID NO:1694 GTCTGAACTAAATGATGAGAGTCTGAACTA

SEQ ID NO:1695 GAGCAAACAATGACGATGACGA

SEQ ID NO:1696 GGAAAGTCATATCCTCCTAAAGAACACTAA

SEQ ID NO:1697 GCAAGACCCTGTCTAAAAAGTAAACTTC

SEQ ID NO:1698 TATCCTGAATATGGATTACTGCAAGGAGAT

SEQ ID NO:1699 ATTCAATGCAGGACTCAGCCAT

SEQ ID NO:1700 TTTTGGATTACGTTTGTGATTTTAAGCAGA

SEQ ID NO:1701 GAAGAGTGAGAGAGACGTGAACAG

SEQ ID NO:1702 GCTATGCTAGATAATGATTACCACAAGCTA

SEQ ID NO:1703 GACACCACCTGGGATGACT

SEQ ID NO:1704 AAAAGGCTAAGTGCACAATCTCCT

SEQ ID NO:1705 GGACGCTATAGGGAGAAGCAAG

SEQ ID NO:1706 TAAGAATGGCCCATTTTGAATAAGGATCA

SEQ ID NO:1707 CTGGAGTGTCCAGACTCATGTG

SEQ ID NO:1708 AATTGATATCAATGAGGCCTCTTATACTGC

SEQ ID NO:1709 CATGAGGCTCTGACTTCCCTT

SEQ ID NO:1710 GCCAAATTCATATGCAAGGCATAATGATAT

SEQ ID NO:1711 GTTGGATAAGCCTCTTATTCATATCCTGAA

SEQ ID NO:1712 GACTTAGTTCTGGTTGAGATGAAAGGA

SEQ ID NO:1713 TGTCTGTAGTCACACACAGGGAA

SEQ ID NO:1714 AACTCTAAGGGCTAAGCCAGAGA

SEQ ID NO:1715 GGGTTGTGAAGCCTTGGATCAT

SEQ ID NO:1716 GCAGTTTGCTTCTTCAGTGAAAAAC

SEQ ID NO:1717 GTGCACATTTTTGTTCCATTTGAAACT

SEQ ID NO:1718 GACTATCCAGGATTGGAATTACACAAGT

SEQ ID NO:1719 ACGTGCATCCTCCCTGATTCTA

SEQ ID NO:1720 GCTCTTTCTCTCTCCTCTTCCCTTA

SEQ ID NO:1721 GTATGAGGTGGCTTTTGTTGTTGTT

SEQ ID NO:1722 CGTTCATAAGAACAAAAGCCTATCATTCTC

SEQ ID NO:1723 GTGTCATTGTAACTCACAGGAGGAT

SEQ ID NO:1724 ATCACCTCTCCTGTCGAAAAGC

SEQ ID NO:1725 ACCTGGGAAGCATTGAAGTAGG

SEQ ID NO:1726 TAAAGCCAGGACAGAGCATGATG

SEQ ID NO:1727 GTTCTCGGAGTGTGTATGCCAA

SEQ ID NO:1728 GATGACGATTTTGATGCCCACAA

SEQ ID NO:1729 TTGTTTTATGCTCAGGGTCAATTTCTTTTT

SEQ ID NO:1730 GATCCAGAAACAACTGGATCAGGT

SEQ ID NO:1731 GTCTCCTTCCTCTTCCTACAGTACTC

SEQ ID NO:1732 TTTGAGAGGAGAGCCCTAAGGT

SEQ ID NO:1733 GGAAAATTGTCTTTTAGGCTACGAAAGATT

SEQ ID NO:1734 AACTTCATGATAGGGAGGCTTGAAG

SEQ ID NO:1735 GAACAGAGTAAAGTAACAACTGATTTCAGG

SEQ ID NO:1736 CCGATGAAGGCTTTGACTGTGT

SEQ ID NO:1737 CGTAGACATTCGGTACAGTTCACAT

SEQ ID NO:1738 CAACCTGAGGAATCACACCTGT

SEQ ID NO:1739 GTTCTGACATAGCATTATCCTTCGGTT

SEQ ID NO:1740 TATCTGACAGCTGTCAGCTTTAATAAGC

SEQ ID NO:1741 CAGCCAACACACGCATTAATGT

SEQ ID NO:1742 TTGTGTGAAGTATCATTCTCCATGAATGT

SEQ ID NO:1743 GTCCAATGACCTGTCCCAGAA

SEQ ID NO:1744 CCCTACTTAAAGTATGTTGGCAGGTT

SEQ ID NO:1745 CCCTCATCTCACTGTTGTGCTTT

SEQ ID NO:1746 ATTTCAATTAATGCTGACAAGTAAAATACCATT

SEQ ID NO:1747 GTCCACAGGGAAGCCTACCAG

SEQ ID NO:1748 TAAGATGCAGCTACTACCCAGCTA

SEQ ID NO:1749 TGGACGACAACCAGAATGAGTG

SEQ ID NO:1750 GGGCTTATGTCTAAGTTTTTCAAGCAC

SEQ ID NO:1751 AGGTTTTGATCATCAGCATACACACA

SEQ ID NO:1752 ATGATGCTTAGTCCAGTAAGTAAATTCAGT

SEQ ID NO:1753 AGAGCAAAGGAGACTGGTGTTTG

SEQ ID NO:1754 GTATGGGTATGGTATGTGTGTTGCT

SEQ ID NO:1755 GCCACCTGGGTGTTTTCTTAAG

SEQ ID NO:1756 CCACATGATGGACTGATAGAATTGTGATAT

SEQ ID NO:1757 GCCTCTGGCTCTTGGAGATGA

SEQ ID NO:1758 GAGCAGCTTTATACTGTAAACTACTGGATT

SEQ ID NO:1759 CGGCCAGAACTGTGAGGAAAATAT

SEQ ID NO:1760 TCTTAGCTCATCACTACTTTCTCCATCATA

SEQ ID NO:1761 GCCTTTCATAATTTTAGAAATCAGAGTGCA

SEQ ID NO:1762 GCCTATGAGGAATTAGAGAGAGAAAATGAG

SEQ ID NO:1763 CTTTTGAAAATGGTTGTTGCTGTGTAAAAA

SEQ ID NO:1764 CCACCTGAGGACTCGCCTAT

SEQ ID NO:1765 TGAAGTTTGGGAAATACCAACACAGTATAT

SEQ ID NO:1766 CCTGAATCATCACCTTTTTCTCCACT

SEQ ID NO:1767 GTCTCTCCATGGGAGACAGGAT

SEQ ID NO:1768 TGCCTTTAAGGCTGGGTCTCTA

SEQ ID NO:1769 TTCTTGGACACCACATGGTATCG

SEQ ID NO:1770 GGCCCTAGAGGTGAAGAAGGAA

SEQ ID NO:1771 CCAATTCCTGCCTGAGCTAGAAC

SEQ ID NO:1772 GCACTGCTGTCTCGTCTAAGTG

SEQ ID NO:1773 GTGCCTACCTTGATGATGATCTTCTC

SEQ ID NO:1774 GGGATTTAGTGGTCCCTGAGAGT

SEQ ID NO:1775 CAACTCATGCCAGTTGGGTCTTA

SEQ ID NO:1776 GAATGAGAAGGACCATGGGTATACTG

SEQ ID NO:1777 CACAGTAATGACAAGACAGGAAGGT

SEQ ID NO:1778 GGATCTTTGGGTAATTTACCTCAGTTCT

SEQ ID NO:1779 CAGTCAGTCGAGGCAACATCAT

SEQ ID NO:1780 CTAGCAGTGATCTGCAGAACCA

SEQ ID NO:1781 CTGAGCACCAAAGCCCTCAGAA

SEQ ID NO:1782 TGCTGAAACAGCTGAAACAGGT

SEQ ID NO:1783 CAAAAACTCACTTGACTAAAAATGGGAACA

SEQ ID NO:1784 TTGTTAGTGACCCTCCTCTTTTGG

SEQ ID NO:1785 CCTCTGTCTGAACTAAGATGTACTCTG

SEQ ID NO:1786 GTCTACCATTTTGCTTGTGCCAT

SEQ ID NO:1787 CACCTCGGCAATTTTGAGATGC

SEQ ID NO:1788 ACAAAGGGAGAAGCTACGTAATGAC

SEQ ID NO:1789 ACTACAAATTACTGTTCCTGTTTATGCCT

SEQ ID NO:1790 TGCAGTATCACAGCACTCTTTAGATAAAC

SEQ ID NO:1791 GCCAGATCATCATTCTTTGTACTAGAATCT

SEQ ID NO:1792 ACAATGAGTTGTGACAATCCCACT

SEQ ID NO:1793 CCCTTTCCTACTAGGATTAAGGTCAGTAAT

SEQ ID NO:1794 TTTTTGCTGAGTAATACGCAAATCCTTAA

SEQ ID NO:1795 AATACTGAGGACATGGGTTTCTAAATATGT

SEQ ID NO:1796 CAAAATGAGTTTTAGAACTTTGCCCTGAT

SEQ ID NO:1797 CTTCTCCGTTCTCTCTCATTATTCCC

SEQ ID NO:1798 GGACAGCGATGTGTGTTATATCCC

SEQ ID NO:1799 GCAGAGAAGATGAACATACACATACTAACA

SEQ ID NO:1800 TGTCATTTCTTCACTGATTGTTAGTTTGC

SEQ ID NO:1801 CAAGAAGTAGCAAGCTGGCTTT

SEQ ID NO:1802 TGTGTGCCCAATTACTGCCTTAG

SEQ ID NO:1803 CTGACCTGGACGACCAGACAGA

SEQ ID NO:1804 CAGCTGTGTAATTGGATGGGTTTTT

SEQ ID NO:1805 CAGCATGCAGTTCTAAGGCTCT

SEQ ID NO:1806 AACACATGGAAACCTTTTAGAAACTGTTTT

SEQ ID NO:1807 CCCTCACCTCTATCCTATGGGA

SEQ ID NO:1808 CCTGTTTTGTCCAAGTAGAAAAAGGATACA

SEQ ID NO:1809 TGCAGCGCGTCAATGACT

SEQ ID NO:1810 GTAAAGGCAGGCCAGCTACACT

SEQ ID NO:1811 CCTGTTGCCCAGATCCTGATC

SEQ ID NO:1812 TACTGAGCCCTTGGTTGAACTTC

SEQ ID NO:1813 AAAAATTATTTTGGCCAGGTTTTCCAAAG

SEQ ID NO:1814 AGTGGGACTCCTGGGCTTATTA

SEQ ID NO:1815 CCAAAGTGAGTTACTACAGAACATCCT

SEQ ID NO:1816 GAGGGAGAGCTGTCTTGTCATG

SEQ ID NO:1817 CCACAAGCAGCACATGGAAAAATT

SEQ ID NO:1818 GCTGGTGTTGTGGGAGAGAGTA

SEQ ID NO:1819 AGACACTCAACGAGCCTCAAAAA

SEQ ID NO:1820 TGTGATCTGTACAGCTTCTCTGGT

SEQ ID NO:1821 GTTGCTACTGCTCATCTCTATCCAC

SEQ ID NO:1822 CCTAATTCAGTGGTCTTTCTGCCTT

SEQ ID NO:1823 GGAAAGAAAACCCTGGGACAACTAA

SEQ ID NO:1824 CAGCATCCCAGGTATGTGCTAC

SEQ ID NO:1825 GATGTCTCATGCTTGGGCTAGA

SEQ ID NO:1826 GGAGTTTGTAGTATGGCTTTGCTCT

SEQ ID NO:1827 AATAATCTCCAGGCTCTGAAGATCAAG

SEQ ID NO:1828 ATGAGAAGAAGGAGCTGCAGAAGAAG

SEQ ID NO:1829 GCAAAAATAGGTGTGGACGTATAAATGTCT

SEQ ID NO:1830 ATCAACTCCTGGACCTGAACTTG

SEQ ID NO:1831 AATGCTTTTTCTGTCCAGAAGTCATTTTT

SEQ ID NO:1832 GCACAACACAGGTTAGGGTTGA

SEQ ID NO:1833 CCAAACACATTCTATGGTTGGGAAAG

SEQ ID NO:1834 CCTCTGGATGGGATTGATGCTC

SEQ ID NO:1835 GTTACGATTAGGAGATGGACATTCTAGG

SEQ ID NO:1836 CATTTCTTACTGAATGAAGCTGCTTGATTT

SEQ ID NO:1837 CTCTCGACCTGACTTATAAAGAAAGCAG

SEQ ID NO:1838 CACTTTACTGTCCTAGGTAAGGACTTTTT

SEQ ID NO:1839 GCTCCACTGTAGCCATAGCAAC

SEQ ID NO:1840 CAAAATGATGAATCCCAGCTCCCATA

SEQ ID NO:1841 CGTCATCTCCGACTTCATCTACCA

SEQ ID NO:1842 CTGAGATAAGCAATCCCGAAAACATG

SEQ ID NO:1843 CACTGCGAGACCAACATCAAC

SEQ ID NO:1844 GGAAATCTGTAGCACACACTCTTAAGATT

SEQ ID NO:1845 ATCCAGGAACCAGCCAGAAACTC

SEQ ID NO:1846 GCAGTCAACTGGAATTTTCATGATTGAATT

SEQ ID NO:1847 AAATGTGATAGCACCTAAGGTGGAG

SEQ ID NO:1848 AAAAGGAAGAATAAGGTTTGGTGTTTTGAA

SEQ ID NO:1849 CCACCAGACACCTTTGTCACAG

SEQ ID NO:1850 ACCTCACACGTTATTTAATAGGACTGTAC

SEQ ID NO:1851 GCACTCTGATGGCGGAAAGA

SEQ ID NO:1852 AGCCTTCTAAATCATGAATGTGGCA

SEQ ID NO:1853 CAGTTTGAATGGTCAATGCGAGT

SEQ ID NO:1854 GCCCAGAAGACTATTTCAGTCTGTT

SEQ ID NO:1855 CTCAAGGCACGGAGGAAGAAGTC

SEQ ID NO:1856 GTTTCATCTTCATGGAGGTACCAGTT

SEQ ID NO:1857 CTTCCTCTGGTGATGGAACCTTG

SEQ ID NO:1858 ATTTTGACCTGACTTGTCAGCTCTT

SEQ ID NO:1859 GCGTAGAAAATGACCTTATTTTGGAGG

SEQ ID NO:1860 CCATCCTGGAGACACCCATCA

SEQ ID NO:1861 CTGGACAATTTTGGAGTTTTCTGAAAAGAA

SEQ ID NO:1862 GGTAGGTGGGAGGACAGCTAAT

SEQ ID NO:1863 TCGCCTCCTTATAAGCAACATTCTTC

SEQ ID NO:1864 CGTGGTACTGATGCTTGTGTGT

SEQ ID NO:1865 CACTAGTAGGCAATTGTCACAACAAAAA

SEQ ID NO:1866 GCTTACAGCATCCTAACGCTGT

SEQ ID NO:1867 GGTGTAGTGTACATTGAGGAGATGAC

SEQ ID NO:1868 GCATCTGCTCTTGCAGAAGCTA

SEQ ID NO:1869 TGGTTCTTTCCGTTATGTAATTATTTCCCA

SEQ ID NO:1870 GCCTTATCTACAGGACCAGTCCTT

SEQ ID NO:1871 ACTGTATTTGTTTATCTGCCATTTCCTCT

SEQ ID NO:1872 CTCCACACAGCAGGTGGAGGAA

SEQ ID NO:1873 CAATGCACCACCTATTTTCAAACATTATTG

SEQ ID NO:1874 GCAGATGGGCCTTTTAAACCAG

SEQ ID NO:1875 TTTATTGGACAGATGTTGTAGAAGACAGA

SEQ ID NO:1876 TGGCAACCTTGCTCTTCGAA

SEQ ID NO:1877 TTTAACTGAGGAAAGCAATTGATAAGACCT

SEQ ID NO:1878 GCTGGGACACAGGTAGGTAGAG

SEQ ID NO:1879 AAAATTCCTCACCTTCAGAGTCCTATTAAT

SEQ ID NO:1880 CCTGTTCTGGGCATCAGATGTAG

SEQ ID NO:1881 CAGAAACCTGGCTTCTCAACTTTT

SEQ ID NO:1882 GTATGTGACAGAGGCTGGGTTTAG

SEQ ID NO:1883 CTCAGCATACTAACAATATGCAAGAGATCA

SEQ ID NO:1884 GTAAGCCATAACTTTATGTCATCCTAGTGA

SEQ ID NO:1885 CAACAGACAGGGAAATCGAGGTT

SEQ ID NO:1886 CTGGAGATAATGCCAAGTTTTTCTTTGTAT

SEQ ID NO:1887 CAACCCATGATACTGAGCAGAGT

SEQ ID NO:1888 CAGTGGTTTCAACAATTAAGAGGAAAAGTT

SEQ ID NO:1889 TATCTGGGATGAGCCGTGATCA

SEQ ID NO:1890 GGACGTTTACTTACTTTTCAAGCCAAAATA

SEQ ID NO:1891 CCCAGGCTTCTAAGTACCCTGA

SEQ ID NO:1892 GACTTTGTATAAAGGCAGAACGTGAAG

SEQ ID NO:1893 ACTCATGACTGTCCTTTCTTTGTTTACAT

SEQ ID NO:1894 CTGAACTCTCTTCCTTTCAACCAAAGAT

SEQ ID NO:1895 GAGGAAAGGTAAATCCACCAATTACCT

SEQ ID NO:1896 GAATTCAAATATGAACCTGCAGCTTGA

SEQ ID NO:1897 CCTAGTAAAGAAATGCATTGTGTTGGGA

SEQ ID NO:1898 CTATCATGGTTAAGAGCTGGAAGCAT

SEQ ID NO:1899 AAAGGTTGACTCTTAGTGTATTTCAACTGT

SEQ ID NO:1900 GCAACCCTAGAATAGCTAAGCCTACTAATA

SEQ ID NO:1901 AATGTAACAGCTTGTTGACCCATTTG

SEQ ID NO:1902 TTCTCCATTAGCCTTTTGGTCTTGTT

SEQ ID NO:1903 AAATCACGTAATCAGCAATGAGTATTCTCT

SEQ ID NO:1904 TCTGTATGTCATTCCTGTTCACACAATTA

SEQ ID NO:1905 TTTCTAATTATTTCTGTGTGGGTGTGTGA

SEQ ID NO:1906 TGAAAATGAAATCTAGTGCCACACAGT

SEQ ID NO:1907 CCCTAGAGGGCCTGAGAGTT

SEQ ID NO:1908 GGTGGCAGCAAATCACACATTC

SEQ ID NO:1909 GAAAGCCCATGGTATAATAAAGCTCTAGT

SEQ ID NO:1910 TGTGTTTTCTTCCTTTTCTTTTCTCTTCCT

SEQ ID NO:1911 ATTGGCTTTTCCTTAATAGTCTATTTCCGA

SEQ ID NO:1912 CTTGCCTTGTATATGTTATCATTTCCCTTG

SEQ ID NO:1913 GGCACAGCACTTGTTTTATAGATCCATAA

SEQ ID NO:1914 GGACTAAGTCCCTACTCGGATTATGA

SEQ ID NO:1915 CATCTCTATGACCTACATAGAAGGCTCA

SEQ ID NO:1916 TGTCATTGATGTAAGAAATGGACAAGTCA

SEQ ID NO:1917 TCAAGTTATGCAGCTTAAAAGTCCTATCTC

SEQ ID NO:1918 TGCATCACACACTTATCATTAAATCCACT

SEQ ID NO:1919 GTTCCTCTGTTACTTGACAAATGTCATTT

SEQ ID NO:1920 CCTCACCTAAAACATCTTTACTACTCACAT

SEQ ID NO:1921 AAGAAGTTGTGAGGTAAGATTACCATTCC

SEQ ID NO:1922 GAGGTGGAACAGTAAGATAGCACTG

SEQ ID NO:1923 GTGTGTGACCAGAATTTTCAAGAAAGAAAT

SEQ ID NO:1924 GGTGGTAGAAAGGAATTAAAATAGCACATT

SEQ ID NO:1925 ATGAGGTCTGTTGGATTTCTTTTCAGTATT

SEQ ID NO:1926 GGGTACACTGCATTCAAACTGTAG

SEQ ID NO:1927 ATTGGTTCTGTCTGACTAACCTCTTTTT

SEQ ID NO:1928 GCAATTGGAATTGCCTTGAATTTTGAAATT

SEQ ID NO:1929 CAAGAGCAGCACTCTTACAGTATTTTCTAT

SEQ ID NO:1930 TGATACCATGTTTTCCTGAATATGGCT

SEQ ID NO:1931 AGCTTTTATTAACGGAACTGGGTTAGAA

SEQ ID NO:1932 CTGCTCCTGTTCTTGAAAGCAC

SEQ ID NO:1933 GATGTTGTCTCCAAGCAAATAAAAACCTTA

SEQ ID NO:1934 AAGAAGTTGTTTGCTGCTCTAAAGC

SEQ ID NO:1935 AATATTTAGAGCAGGGCAGATAAATCAGTT

SEQ ID NO:1936 CTTCCCATTCAACTTCATCTCCTCA

SEQ ID NO:1937 GGTAGAACTGTCTCATTGGACATAATTGTT

SEQ ID NO:1938 GTGAAAATGGCTGAGAACTTGCT

SEQ ID NO:1939 GCATGTATTAAACATTTGTGCTTTATGGTG

SEQ ID NO:1940 CCTTTTCTGAGTGTTTCTTGCCA

SEQ ID NO:1941 CTTTCCCTAGGCAGCTGTAAGTTTATATTT

SEQ ID NO:1942 TGACAAAGTGAGAGGTGAGACAGA

SEQ ID NO:1943 GAAACCACACCTATTACTCTGCTCTT

SEQ ID NO:1944 CTGAGTGGGTCTATAATACATTTTCTTGCA

SEQ ID NO:1945 CTTTTCAAGTGAGGTAACCAACAAAAGG

SEQ ID NO:1946 ACCTGTTTTAGGCTTGTAAGAGTCTTC

SEQ ID NO:1947 ATTCTGAGGCCTCGCAAGTAC

SEQ ID NO:1948 GCATTGGAACACACGTGCTTTT

SEQ ID NO:1949 ACCTGGACCACCTGTACTCTC

SEQ ID NO:1950 TCTCCTGCACAAACTGGATGTC

SEQ ID NO:1951 GTATTTTCATTGTTCCACCTCCATTCTG

SEQ ID NO:1952 GCACTGGGAGTTCTCCTCCTT

SEQ ID NO:1953 TGGCATGGAAGATATCTACAAGAGAGA

SEQ ID NO:1954 GTGTCCCAGTAGGGCTTTTCTC

SEQ ID NO:1955 GCCCTTCTCTCACAAGGTTACA

SEQ ID NO:1956 AGCGGATTCTGCAGCAACA

SEQ ID NO:1957 CCAAATCTGAGGAAGTGTCTATACTGTG

SEQ ID NO:1958 CAACAGCAGGTGCTGAACATTC

SEQ ID NO:1959 ATTTAGTTTCAAAGGAAGACAGTGATTCCT

SEQ ID NO:1960 CGACCTCTGCATCAACTGCTATAA

SEQ ID NO:1961 TGTCAACCTCAGAGCTTCCCTA

SEQ ID NO:1962 GTTACCATTTTCTTATGGAATCTGGCATTA

SEQ ID NO:1963 AGGAATGCTTAGATTTTCAAAAATCCTGAA

SEQ ID NO:1964 GCCAGAATCACTTATTTAAATAACCACCAA

SEQ ID NO:1965 GGCCTATTATTCACATAAAAAGGCATTCAA

SEQ ID NO:1966 CTCTCTTCGTCTCTCAACCACTAG

SEQ ID NO:1967 CTATCCAGTGCCTAGCATATGGTAG

SEQ ID NO:1968 GGAAGAGGGAAGCTCTTCAGTC

SEQ ID NO:1969 ACCGTAACCTTGTACATTCAAAAAGAGTTA

SEQ ID NO:1970 CCATTGGTTAGTCCCAATTCTGTAGAG

SEQ ID NO:1971 CGTTAGTCAGGAAGTGTAGTACACAATAAT

SEQ ID NO:1972 ACCAATACCATGTTATTAGGATGACACAAA

SEQ ID NO:1973 AGCTTGGTATTGCCATGTTTCTATTAGT

SEQ ID NO:1974 GCTCTAATGATTCTGCCTAAATCCAAATCT

SEQ ID NO:1975 GGAGACAGAAAGACTTGCCTTTATCTATTT

SEQ ID NO:1976 GTGAGCATGCAAAAGGAGAACAA

SEQ ID NO:1977 AAGAATTTCCAGTGACATTTGTAAATGACG

SEQ ID NO:1978 AATGAAATCACAATAATGGCAACTTCTGAG

SEQ ID NO:1979 AAATCTAGCATACCCAGGATGATATCCT

SEQ ID NO:1980 GAGCAGGACCTAAAAATGGTGTATTTC

SEQ ID NO:1981 GAAGTTCTTGAGGACCAGAACTATCAC

SEQ ID NO:1982 GATGGAAGTGTTGAATGTGTCTGTC

SEQ ID NO:1983 GAGGAGAGCCAGTACGACTCT

SEQ ID NO:1984 GGCAATGAATGACAGACTCTATGACATTTA

SEQ ID NO:1985 GATTCTTGAGCCCAAGCATGTTC

SEQ ID NO:1986 TGTTTCCCTTAGAAACAGAGTTTGGAAATA

SEQ ID NO:1987 GGCCTTGGCTGACATCCTTATT

SEQ ID NO:1988 CAGAACCTACAGATAGACCACCTATACTAT

SEQ ID NO:1989 AAGGGAGGTCCTAAATTCATTTTCTGG

SEQ ID NO:1990 CGATGGTCAGAAAGATTGTGAGGATG

SEQ ID NO:1991 GCTCTAAACAGGTAGGAAAATCATACTTCT

SEQ ID NO:1992 GAAAGAAAAACACACTGTCCTTCAAGTA

SEQ ID NO:1993 GGAACAGGTATTTAGCATTGCAGCTA

SEQ ID NO:1994 GCCCATGAGAAGGTGACATTCTG

SEQ ID NO:1995 TCATTTCCCATGTTTTCTGTGGTCA

SEQ ID NO:1996 CAACAGGTCAAGGTAGACAGTCA

SEQ ID NO:1997 GCTGGGCTTTTCATTAAGCAAATAGAC

SEQ ID NO:1998 ATTTCTAACATGCTCCACAGAACAGT

SEQ ID NO:1999 CGGACACGTGACGTACTCAATT

SEQ ID NO:2000 GGAAAATGAAACGGCTGTGTGAA

SEQ ID NO:2001 CAATGAATGTGCTCCGGTGATAAC

SEQ ID NO:2002 CAAAGGTCTGCTAGCTTGTCCTAG

SEQ ID NO:2003 GGCAGGATGCAACAGATAGTGG

SEQ ID NO:2004 CAATGCAAGGCCTCTTCTTTGAAT

SEQ ID NO:2005 GCCAAACCCAAAGAAAACAATTTAAAGAT

SEQ ID NO:2006 ATGCATAATCAATAAAACATCATGTGGCAA

SEQ ID NO:2007 TGGTTGAGATACAATAAGGGTGTGAAAAA

SEQ ID NO:2008 TAGGTGGCTATGCCACCTTTTATTAAAA

SEQ ID NO:2009 ACAAAATCTGACAATAGACATTTTAGTCTACGA

SEQ ID NO:2010 CCATGCATTATTTTAAAACCTTCCCTTCTT

SEQ ID NO:2011 ACATCACACTCTAACTCCATAAATATGCAC

SEQ ID NO:2012 CAGGGAAAAAGCATTTTATTAACATCCTGA

SEQ ID NO:2013 AAATTCTTACTGCACAGGTTATTGCATC

SEQ ID NO:2014 TGAACAGGCACCCAATATATAAATGTTTGT

SEQ ID NO:2015 GGAAAGAGGTGGTTTGAGGCTT

SEQ ID NO:2016 TCTTACTTCCTACTTTAACAAGCAAAGCAT

SEQ ID NO:2017 ATTTCCAACCAGTGTTTATCCGAGT

SEQ ID NO:2018 GCCTTTTTGGTATGTTAAAGAAGAGACAGT

SEQ ID NO:2019 TACCCTGCTGCTCTCGAGTA

SEQ ID NO:2020 GGCCCTCAAATCTCATTCTACTTCT

SEQ ID NO:2021 GTCTCTCAAGCAAGTGCTATTTGTTTTT

SEQ ID NO:2022 ACAACTTCAGGACATAGGCTGTTATTTTTA

SEQ ID NO:2023 GGATTCACCATTCAGTTTCTACTTTTCAAA

SEQ ID NO:2024 GAACCCTTGCTTGCAATAGGTG

SEQ ID NO:2025 GGGCTGGACTTTAAATACAGGGT

SEQ ID NO:2026 ACTTTCTGCTCTTGTCTTTCAGACTTC

SEQ ID NO:2027 CACATCAAGCCTACACTAAAATTCCATG

SEQ ID NO:2028 CCAACTCCATTTCCTTTGCTTCCT

SEQ ID NO:2029 ACAGAGTAGATGCTGACTTTCAAATGTT

SEQ ID NO:2030 GGTTTCATGAGTGCAGGGAGAA

SEQ ID NO:2031 TGGTATCTGGGCTTTAGACTGACT

SEQ ID NO:2032 GAGAAAGGTAAGGGTCAGTGCTTAAG

SEQ ID NO:2033 GGGATGTGTTCTCAAAATAACTTCAACT

SEQ ID NO:2034 GGGTTAACCTTCCTCCTCACT

SEQ ID NO:2035 AACTCCAACACAAAGGTGGGAA

SEQ ID NO:2036 ATTTTGAGGCCTAAATTCATCGGATCT

SEQ ID NO:2037 ATTTTGAAAACCCTTTATTCGGTTTCTCAG

SEQ ID NO:2038 CCTTCAGGCCTAACCCAAACAA

SEQ ID NO:2039 GGTGAGCTCTTCCTCAAGCT

SEQ ID NO:2040 TGCCTCATTATTTTGTTTCCCTAAAGTAGA

SEQ ID NO:2041 CCTCATCTTGGGCCTGTGTTAT

SEQ ID NO:2042 CGCGGTGGCTTTGAAGAG

SEQ ID NO:2043 GACTCCAAGCCTACGCAGAG

SEQ ID NO:2044 CAAGACTGAACATTAATCTTACCATCTCCT

SEQ ID NO:2045 TGGCGGTGCACACTATTCTG

SEQ ID NO:2046 CCCTGAAAACATGCTAATTTGTACATTTCT

SEQ ID NO:2047 GTAAGCCAGAGAGTTGGGAGAGA

SEQ ID NO:2048 AACTTGAATTTCACGCATGCTGTT

SEQ ID NO:2049 CAGTTAACAACCAAATCTTTCTTCAGGAAT

SEQ ID NO:2050 AAATTTACTTGCACTATTTGTCTTGTGTGT

SEQ ID NO:2051 AGCACTCTCAAAGAGGCCAAG

SEQ ID NO:2052 CCCACCCTCCACTTTAACATTTAGT

SEQ ID NO:2053 AAATGTGAGACTGATGTCAATGAGTGT

SEQ ID NO:2054 CTCCCAGAGAATCTGTAAACAAAATCAGTA

SEQ ID NO:2055 CGCCGACTTCATGAGCAAC

SEQ ID NO:2056 CTGTTGAAAATACCGTGGTGTGTT

SEQ ID NO:2057 GAGGCTCTGATCCACTCCTTTC

SEQ ID NO:2058 ACATGTGCATGTGTGTATCATTTTTCTTC

SEQ ID NO:2059 GGGTAATAGAACGCAGGCG

SEQ ID NO:2060 CCTCTGCTTCTCTCTTCCTCATTTC

SEQ ID NO:2061 GGTACGATAACCGGGAGATAGTG

SEQ ID NO:2062 GTCTTACCACTCCATCCCATGT

SEQ ID NO:2063 CCCAGGTAGAAACTTTTATCTATTCTGAGA

SEQ ID NO:2064 CTGGGTTGAAAAGTTGTAAAACTGAAAACT

SEQ ID NO:2065 CCTTGATCTCCACCTGGTTGAAG

SEQ ID NO:2066 GCAAGAGATTGAATCGACGTTGAGT

SEQ ID NO:2067 CGCCCTTACTAGGACATGTCCA

SEQ ID NO:2068 CACTAAGCTGGTACATGTGTACAGA

SEQ ID NO:2069 CATGTGGACTGGAGCTCTGTC

SEQ ID NO:2070 CTTGTTGTCAGCAGAATTGTGTAGATT

SEQ ID NO:2071 CACTTAAATCCTCCTTTAACTCACTACCAA

SEQ ID NO:2072 CAGAATGATGCCTAGTTTATTATGAGCCTA

SEQ ID NO:2073 CCAATGACTTGTGGGTGGTTGT

SEQ ID NO:2074 CTAGAGGTCACTCCAGAGGAGTT

SEQ ID NO:2075 CCTGCAGCCTTTTGATTTCCCTAA

SEQ ID NO:2076 ACATGTTTTTATGTCTTCATAGGATTAAATCGG

SEQ ID NO:2077 CCCGGTCTTTGAATCTAGTCCA

SEQ ID NO:2078 CAGTCGTTAAAGTTCTTGCAAGTGATG

SEQ ID NO:2079 AGTATTCCATTCTGTCTGGCAATGAT

SEQ ID NO:2080 CCTTTCAGCCAGTTCACTATTAACTTCAA

SEQ ID NO:2081 CCTCAACCCAGATCGCAGATTTAAA

SEQ ID NO:2082 TTTACCGTCCAAGTGCATGACA

SEQ ID NO:2083 GATAAGGGCAGTCCACCAATGA

SEQ ID NO:2084 AATTAGTTATAAGGAAAGCACCAATGGGAT

SEQ ID NO:2085 ACATGACTTTCGTTCATGGTTCTTTAAG

SEQ ID NO:2086 GGTAGCTTGGGTTCTTCATCAAGAAA

SEQ ID NO:2087 CCCTTGTTTTTGTAAATTGGAAGGCTAATA

SEQ ID NO:2088 GGTACATGTAAAAAGTCTGCACGTTAATT

SEQ ID NO:2089 TTTATTGAGTAAATCTCAGGCCATCGTT

SEQ ID NO:2090 TGTTTTCCAGCTTTGTGGAAAAGG

SEQ ID NO:2091 AGCTACAGATGGAGAAAATTTTGCCA

SEQ ID NO:2092 GTGAAAGTCTTAGGTGCAAATAGCAA

SEQ ID NO:2093 AGAGTACATTCTCGGAGACTTTTGC

SEQ ID NO:2094 ATTTCAGTCATAGTGGGAAAATGTGGT

SEQ ID NO:2095 CGTAAGATGATTAGTCGGAAAAAGAAGCA

SEQ ID NO:2096 AGTCCTCAAGTTGGTAATGGTTTCAG

SEQ ID NO:2097 CTCAACAAAACAACTTCTGCACAAGATTA

SEQ ID NO:2098 CAGTGCCATAGACAAAGGTGATTTTATTTT

SEQ ID NO:2099 CGAGCTCTAATGACAAGCTCATGTAC

SEQ ID NO:2100 CAAACCAGTCTGTGCTTCATTCA

SEQ ID NO:2101 GCATGAGGAAGAAGATGCTCACTTTTTATA

SEQ ID NO:2102 CATCTCATAGACAGGAATAACCTTGTTGTT

SEQ ID NO:2103 TTTAGATGTAAATGTGTGAATGTCTGTCCA

SEQ ID NO:2104 AGCCACTGTACTGGGCTTTAATC

SEQ ID NO:2105 CTTAAGGTGTAACAAGTGAAAAGCAATTTG

SEQ ID NO:2106 GCAGCTTGGTAGCATTTTGAAAACAA

SEQ ID NO:2107 TGTGACAGGGCTTTTCAGCTTAAA

SEQ ID NO:2108 CTTGGCCTGTTTTTGACATCTACC

SEQ ID NO:2109 CACCTGGGCCAAAATCAGTTTC

SEQ ID NO:2110 TACTGTTATAAAGGTATGCAAGGGATAGGT

SEQ ID NO:2111 GCTAAGTAATTTCAGCTTTTGTTACTTGCT

SEQ ID NO:2112 CAGAGGAAGTATTGGTTGTAGAGAATGATC

SEQ ID NO:2113 TGAGTCTGACTACAACCTAATTCTGTCA

SEQ ID NO:2114 CCACTTCTGTAGAATGGGTTGCA

SEQ ID NO:2115 TGGGTTAATGCTTGCTGAATTGGA

SEQ ID NO:2116 CTGAACACTTCTGAAAAGTTGGCTAAAATA

SEQ ID NO:2117 ATAATTTTGTTAGTCAGGGCTCCCAAT

SEQ ID NO:2118 AGCCTAGGGACCAGCTGATATC

SEQ ID NO:2119 TGACATTGGTAGGAGCTATATTTGTTAAGG

SEQ ID NO:2120 CAAAGGAAGGAGAGAGTTTCTAGGAG

具体的，本发明实施例的试剂盒包括样本处理试剂盒、目的片段扩增试剂盒和文库建库试剂盒。

其中，样本处理试剂盒包括样本处理试剂、样本质控试剂和样本PCR扩增试剂；

目的片段扩增试剂盒包括目的片段扩增试剂、目的片段纯化试剂和片段纯化产物定量试剂；

文库建库试剂盒包括PCR产物末端修补和末端加polyA反应液、连接barcode接头反应液、barcode产物纯化试剂、文库富集试剂、文库纯化试剂、文库质控试剂。

本发明实施例还涉及该靶向测序试剂盒的使用方法，具体包括样本处理、PCR扩增、目的片段扩增、目的片段纯化、目的片段纯化产物定量、文库建库和测序的步骤。

具体的，文库建库包括PCR产物末端修补和末端加polyA、连接barcode接头、barcode产物纯化、文库富集、文库纯化的步骤。

本发明实施例试剂盒的使用方法具有步骤简单、易操作的优点。

本发明实施例还涉及一种测序方法，具体包括样本处理、PCR扩增、目的片段扩增、目的片段纯化、目的片段纯化产物定量、文库建库和测序的步骤。

下面通过具体实施方式对试剂盒、试剂盒的使用方法展开进一步详细说明。

实施例1靶向测序试剂盒的组成

1、样本处理试剂盒：

(1)样本处理试剂：可依据样品的不同采用现有市售样本处理试剂，具体的，石蜡样本可使用QIAGEN石蜡组织DNA提取试剂盒，按照相关石蜡抽提试剂盒说明书进行操作，得到完整的DNA；血液样本可使用QIAGEN全血DNA提取试剂盒，按照相关全血抽提试剂盒说明书进行操作，得到完整的DNA。组织样本可使用QIAGEN组织DNA提取试剂盒，按照相关组织抽提试剂盒说明书进行操作，得到完整的DNA。

其中，在样本处理的过程中，还包括样本质控试剂，其中样本质控试剂盒的组成如表1所示：

表1

其中，质控引物和探针的序列如表2所示：

表2

其中，DNA质控阳性对照的制备方法为：使用QIAGEN全血DNA提取试剂盒对正常人的全血进行抽提，并使用Qubit dsDNA HS Assay Kits试剂盒对抽提完的DNA进行定量，然后根据所定浓度，将抽提所得的DNA稀释至50ng/μl。

2、目的片段扩增试剂盒：

目的片段扩增试剂盒的组成如表3所示：

表3

其中，Mix反应液为市售反应液；XP Kit和Qubit dsDNA HSAssay Kits均为市售试剂盒。

Barcode 1-10反应液是10组不同barcode的反应液，每组反应液都含有核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物，每条引物的浓度为200μM，体积为0.225μL。

其中，Barcode 1反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列TCGCCTTA；

Barcode 2反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列CTAGTACG；

Barcode 3反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列TTCTGCCT；

Barcode4反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列GCTCAGGA；

Barcode 5反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列AGGAGTCC；

Barcode 6反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列CATGCCTA；

Barcode 7反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列GTAGAGAG；

Barcode 8反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列CAGCCTCG；

Barcode 9反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列TGCCTCTT；

Barcode 10反应液在每条核苷酸序列SEQ ID NO:1～SEQ ID NO:2120的引物的5’端连接有核苷酸序列TCCTCTAC。

除以上5’端连接有核苷酸序列外，Barcode 1-10反应液在每条核苷酸序列SEQ IDNO:1～SEQ ID NO:2120的引物的5’端还可连接其他的核苷酸标记序列，具体标记序列可根据以下两个原则进行设计：

(1)保证测序过程一个Cycle中红黄蓝绿四种光的均一性良好，防止发生在同一个Cycle中只存在同一种光。

(2)保证每个Index的测序复杂度，防止在测序过程中，由于测序错误导致在数据分析过程中无法区分Barcode的情况。

3、文库建库试剂盒

文库建库试剂盒的组成如表4所示：

表4

其中，上述制剂和试剂盒均为市售产品。

进一步可选的，上述市售产品可选用表5所示的具体产品。

表5

实施例2试剂盒的使用方法

一、样本处理和质控

1.样本处理：

石蜡：按照相关石蜡抽提试剂盒说明书进行操作，得到完整的DNA。可使用QIAGEN石蜡组织DNA提取试剂盒。

血液：按照相关全血抽提试剂盒说明书进行操作，得到完整的DNA。可使用QIAGEN全血DNA提取试剂盒。

组织：按照相关组织抽提试剂盒说明书进行操作，得到完整的DNA。可使用QIAGEN组织DNA提取试剂盒。

2.样本质控：

从实施例1的试剂盒中取出DNA质控PCR反应液、DNA质控阴性对照、DNA质控阳性对照，室温融化并振荡混匀后，短暂离心数秒。

梯度模板配制：取5μL试剂盒中的DNA质控阳性对照C1(浓度为50ng/μL)加入20μL的ddH₂O中(按1：4)稀释，即得DNA质控阳性对照C2(浓度为10ng/μL)；取5μL>2O中(按1：4)稀释，即得DNA质控阳性对照C3(浓度为2ng/μL)；取5μL>2O中(按1：4)稀释，即得DNA质控阳性对照C4(浓度为0.4ng/μL)。

反应液体系1人份均按如下配制：

22μLDNA质控PCR反应液+3μL模板(PCR管数＝样本数+DNA质控阴性对照+4个DNA质控阳性对照(C1-C4))，盖紧管盖，快速离心10秒，将其移至检测区。

3.样本PCR扩增：

ABI 7300、7500荧光PCR检测仪扩增条件：42℃，5min；94℃，3min；(94℃，15sec；60℃，60sec)40个循环。反应体系为25μL。在PCR循环第二步60℃时收集荧光信号。检测通道为：FAM-TAMRA。参比荧光设定为none。

其中，样本PCR扩增的反应体系组成如表6所示：

表6

组成体积DNA质控PCR反应液22μL样本3μL共计25μL

其中，DNA质控PCR反应液中含有Mg²⁺2～3mM、dNTP>

Stratagene Mx3000p、罗氏480荧光PCR检测仪(注意：对于罗氏480荧光PCR检测仪，将其升降温速率设为1.1)扩增条件:42℃，5min；94℃，3min；(94℃，45sec；60℃，80sec)40个循环。反应体系为25μL。在PCR循环第二步60℃时收集荧光信号。检测通道为:FAM，参比荧光设定为none。

检测：(1)基线的确定：软件默认为3-15个循环的平均荧光信号为基线。在实验中，一般选择曲线波动较小，较稳定的那段作为基线，用户可根据实际情况自行酌情调整。终点要避免覆盖信号已经开始有明显增长的地方。且以起点与终点之间最好能间隔8个循环以上为原则。

(2)阈值的确定：在阴性对照无扩增的情况下，阈值设定在无典型扩增曲线样本的最高点，且阴性对照未检出为原则，确定起始阈值。

(3)计算机自动处理和分析数据。

【结果分析】

有效性判定具体如表7所示：

表7

结果判定：

DNA质控阳性对照C1-C4设定为50ng/μL、10ng/μL、2ng/μL、0.40ng/μL；在扩增结束后，用获得的标准曲线，得到各样本中基因组DNA的有效浓度。

如果样本CT值≥38或显示“Undet”，则判定其标本基因组DNA片段有效浓度低于最低检出极限。

如果样本CT值<38，则按其标准曲线计算得到标本基因组DNA片段有效浓度。

若标本基因组DNA片段有效浓度大于10ng/μL，则继续实验。反之，则重新起取样，进行抽提。

标本稀释：

各标本根据上述标定后的DNA有效浓度，逐级稀释至10ng/μL，作为目的片段扩增的模板。

二、目的片段扩增过程

1.目的片段扩增试剂准备：从试剂盒中取出Barcode 1-10反应液、Mix反应液，室温融化并振荡混匀后，短暂离心数秒，反应液体系1人份均按如下配制：12.5μL Mix反应液+7.5μL Barcode 1-10反应液。将上述配制好的PCR预混液，分别按每管20μL分装于各PCR管内。

2.加样：从稀释好的样本DNA液(定量为10ng/μL)中取5μL，加至装有上述相应PCR预混液的PCR管中，盖紧管盖，短暂离心，将其移至检测区。

3.第一轮PCR扩增：

Effendorf普通PCR仪扩增条件:95℃5min(95℃15sec，55℃25sec，72℃40sec)35cycles，72℃5min。反应体系为25μL。

反应体系的具体组成如表8所示：

表8

4.PCR产物纯化

(1)试剂准备：从试剂盒中取出XP Kit，充分振荡混匀后，短暂离心数秒，放置室温30分钟。

(2)PCR产物纯化：在上述每管PCR产物中，均加入30μL的XPBeads，充分振荡混匀后，短暂离心数秒，放置室温5分钟。将PCR板放置磁力架上3分钟，待液体澄清后，吸弃每管中所有液体，加入100μL 80％的酒精，等待30秒，再吸弃每管中所有液体，接着加入100μL 80％的酒精，等待30秒，再吸弃每管中所有液体，放置室温干燥3分钟后，将PCR板从磁力架上取下，在每管中加入25μL的洗脱液，充分振荡混匀后，短暂离心数秒，放置室温5分钟。最后，将PCR板放置磁力架上3分钟后，收集每管PCR纯化产物20μL。

5.PCR纯化产物定量：Qubit 3.0定量：

(1)配制工作溶液：

从Qubit dsDNA HS Assay Kits中取出Qubit dsDNA HS Reagent和Qubit dsDNAHS buffer试剂，振荡混匀后，短暂离心数秒，反应液体系1人份均按如下配制：199μL QubitdsDNA HS buffer+1μL Qubit dsDNA HS Reagent(配制数＝标本数+2)

(2)配制标准溶液：

从Qubit dsDNA HS Assay Kits中取出Qubit dsDNA HS standard#1和QubitdsDNA HS standard#2试剂，振荡混匀后，短暂离心数秒，配制标准溶液方法如下：

Standard 1溶液：190μL工作溶液+10μL Qubit dsDNA HS standard#1

Standard 2溶液：190μL工作溶液+10μL Qubit dsDNA HS standard#2

(3)配制检测溶液：

检测溶液(每个测试)按如下配制：199μL工作溶液+1μL上述纯化后的文库。

(4)检测

将配制好两个标准溶液和检测溶液，振荡混匀后，短暂离心数秒。室温静置3-5min后，使用Qubit 3.0仪器测量文库浓度。

三、文库建库过程

根据Qubit的定量结果，将每管纯化后的产物稀释至2ng/μL，按照一定比例混合成50μL中含有PCR产物100ng。

1.PCR产物末端修补和末端加polyA

从KAPA Hyper Prep Kit Illumina Platforms中取出KAPA End Repair&A-Tailing Buffer和KAPA End Repair&A-Tailing Enzyme，

按照表9配制PCR产物末端修补和末端加polyA的反应液：

表9

试剂名称体积KAPA End Repair&A-Tailing Buffer7μLKAPA End Repair&A-Tailing Enzyme3μLPCR产物混合物50μL总共60μL

末端修补程序：Effendorf普通PCR仪扩增条件：20℃20min，65℃30min。

2.连接barcode接头

从KAPA Hyper Prep Kit Illumina Platforms中取出Ligation Buffer、DNALigase。

并且从PKR Y Type Adapter Kit Index中取出PKR Y Type Adapter Kit IndexX。

按照表10配制连接barcode接头的反应液：

表10

连接barcode程序：Effendorf普通PCR仪扩增条件：20℃、30min。

4.连接barcode产物的纯化

将上述连接barcode产物转移至1.5mL管，并加入88μL的XPBeads，充分振荡混匀后，短暂离心数秒，放置室温5分钟。将1.5mL管放置磁力架上3分钟，待液体澄清后，吸弃每管中所有液体，加入200μL 80％的酒精，等待30秒，再吸弃每管中所有液体，接着加入200μL 80％的酒精，等待30秒，再吸弃每管中所有液体，放置室温干燥3分钟后，将1.5mL管从磁力架上取下，在每管中加入20μL的洗脱液，充分振荡混匀后，短暂离心数秒，放置室温5分钟。最后，将1.5mL管放置磁力架上3分钟后，收集每管纯化产物20μL。

5.文库的富集

从KAPA Hyper Prep Kit Illumina Platforms中取出2X KAPA HiFi HotStartReadyMix和10X Library Amplification Primer Mix。

按照表11配制文库富集的反应液：

表11

试剂名称体积2X KAPA HiFi HotStart ReadyMix25μL10X Library Amplification Primer Mix5μL未扩增的文库20μL总共50μL

文库富集程序：Effendorf普通PCR仪扩增条件：98℃45sec，(98℃15sec，60℃30sec，72℃30sec)3cycles，72℃1min。

6.文库的纯化

将上述文库富集产物转移至1.5mL管，并加入50μL的XPBeads，充分振荡混匀后，短暂离心数秒，放置室温5分钟。将1.5mL管放置磁力架上3分钟，待液体澄清后，吸弃每管中所有液体，加入200μL 80％的酒精，等待30秒，再吸弃每管中所有液体，接着加入200μL 80％的酒精，等待30秒，再吸弃每管中所有液体，放置室温干燥3分钟后，将1.5mL管从磁力架上取下，在每管中加入20μL的洗脱液，充分振荡混匀后，短暂离心数秒，放置室温5分钟。最后，将1.5mL管放置磁力架上3分钟后，收集每管纯化产物20μL。

四、文库质控过程

1.Qubit 3.0定量：

(1)配制工作溶液：

从Qubit dsDNA HS Assay Kits中取出Qubit dsDNA HS Reagent和Qubit dsDNAHS buffer试剂，振荡混匀后，短暂离心数秒，反应液体系1人份均按如下配制：199μL QubitdsDNA HS buffer+(配制数＝标本数+2)

(2)配制标准溶液：

从Qubit dsDNA HS Assay Kits中取出Qubit dsDNA HS standard#1和QubitdsDNA HS standard#2试剂，振荡混匀后，短暂离心数秒，配制标准溶液方法如下：

Standard 1溶液：190μL工作溶液+10μL Qubit dsDNA HS standard#1；

Standard 2溶液：190μL工作溶液+10μL Qubit dsDNA HS standard#2。

(3)配制检测溶液：

检测溶液(每个测试)按如下配制：199μL工作溶液+1μL上述纯化后的文库。

(4)检测

将配制好两个标准溶液和检测溶液，振荡混匀后，短暂离心数秒。室温静置3-5min后，使用Qubit 3.0仪器测量文库浓度。然后根据Qubit 3.0测得的数据，将纯化后的文库稀释至0.75ng/μL，用于Aglient2100的定量。

2.Aglient 2100定量：

(1)准备Gel-Dye Mix溶液

i.从Agilent High Sensitivity DNA Kit中取出High Sensitivity DNA dye和High Sensitivity DNA gel matrix，将其放置室温30分钟。

ii.取High Sensitivity DNA dye 15μL加入到High Sensitivity DNA gelmatrix中，振荡混匀后，转移至spin filter中，以2240g的离心速率，离心10分钟，得到gel-dye Mix溶液。

(2)加样

i.将新的High Sensitivity DNA chip置于CHIP Priming Station上。

ii.High Sensitivity DNA chip如图1所示，在图1所示的A孔内加入9μL的gel-dye mix。

iii.盖上CHIP Priming Station盖子，将针筒安装在CHIP Priming Station上，并且将活塞调至1mL刻度线。

iv.将活塞缓缓推至针筒底部，保持1min后，松开，等待5秒，查看活塞刻度线是否回到0.7mL以上的位置。

v.打开CHIP Priming Station盖子，在如图2所示的三个B孔内，分别加入9μL的gel-dye mix。

vi.然后在如图3所示的没有加过任何液体的12个C孔(环形所示的区域内)内加入5μL的Marker，如下图所示：

vii.在图4所示的C1孔，加入1μL的High Sensitivity DNA ladder。

viii.在图5所示的除C1孔外的其余11个C孔内分别加入各纯化后的文库(1ng/μL)1μL。

ix.然后将加样后的芯片，放置芯片振荡器上，以2000-2400rpm的速度，振荡一分钟，最后将芯片放在Agilent 2100Bioanalyzer Instrument，进行检测。

(3)检测：根据检测结果得知文库浓度(nmol/L)，然后将每个文库均稀释至4nmol/L。

(4)混合文库：取上述各浓度为4nmol/L的文库10μL，等体积混合，振荡混匀后，短暂离心数秒。

五、Illumina上机

1.MiSeq上机浓度稀释

取上述产物4nmol/L 5μL，与0.2mol/L的NaOH 5μL振荡混匀后，短暂离心数秒，放置室温5min，加入990μL的HT1的液体，振荡混匀后，短暂离心数秒。将20pmol/L上述混合液稀释至8pM上机浓度，最后根据仪器使用说明书进行上机。

2.NextSeq 500上机浓度稀释

取上述产物4nmol/L 5μL，与0.2mol/L的NaOH 5μL振荡混匀后，短暂离心数秒，放置室温5min，依次加入5μL 0.2mol/L的Tris-HCL、985μL的HT1的液体，振荡混匀后，短暂离心数秒。将20pmol/L上述混合液稀释至1.5pM上机浓度，最后根据仪器使用说明书进行上机。

3.MiniSeq上机浓度稀释

将上述产物稀释至1nmol/L，取5μL，与0.1mol/L的NaOH 5μL振荡混匀后，短暂离心数秒，放置室温5min，依次加入5μL 0.2mol/L的Tris-HCl、985μL的HT1的液体，振荡混匀后，短暂离心数秒。将5pmol/L上述混合液稀释至1.5pM上机浓度，最后根据仪器使用说明书进行上机。

六、数据分析

1.处理原始测序信号文件，并且分离标本数据

使用bcl2fastq2(Version 2.20.0.422)对原始测序信号文件进行区分barcode，分离标本数据，每个标本得到对应的Fastq文件便于后续数据分析。

2.数据质控

采用Illumina测序平台的双端测序模式对样本进行高通量测序，测序数据以fastq文件格式储存，其中包含测序序列的序列信息和测序质量信息。一般情况下，测序序列5’端前几个碱基错误率较高，3’端碱基错误率随着序列延伸而不断升高。根据测序数据的低质量碱基集中于末端的分布特点，利用Trimmomatic(Version 0.36)软件对测序数据3端动态去除接头序列片段和低质量片段，得到干净的序列片段用于后续分析。

3.数据比对及变异检测、变异注释

通过质控的有效测序数据利用BWAmem算法比对到参考基因组，比对结果经过samtools进行排序，然后使用samtools mpileup进行变异位点的检测，通过Annovar软件对call出来的变异位点进行注释，最后根据生物信息学数据库(refGene、cytoBand、genomicSuperDups、cosmic、clinvar、SNP、ljb26_all、ExAc)对变异位点进行筛选。

实施例3

采用实施例1的试剂盒对3个待测样本进行检测，得到突变比例如表12所示：

表12

样本编号基因名氨基酸突变信息突变比例3632MYD88MYD88:NM_002468:exon5:c.T794C:p.L265P45.96％3451MYD88MYD88:NM_002468:exon5:c.T794C:p.L265P31.15％3460MYD88MYD88:NM_002468:exon5:c.T794C:p.L265P2.78％

并通过数字PCR加以验证，数字PCR的检测结果如图6、图7和图8所示。

3632数字PCR的结果如图6所示，如图6可知，标本3632MYD88L265P的突变比例为42.82％，与本申请试剂盒获得结果一致；

3451数字PCR的结果如图7所示，如图7可知，标本3451MYD88L265P的突变比例为：33.66％，与本申请试剂盒获得结果一致；

3460数字PCR的结果如图8所示，如图8可知，标本3460MYD88L265P的突变比例为：2.09％，与本申请试剂盒获得结果一致。

本申请虽然以较佳实施例公开如上，但并不是用来限定权利要求，任何本领域技术人员在不脱离本申请构思的前提下，都可以做出若干可能的变动和修改，因此本申请的保护范围应当以本申请权利要求所界定的范围为准。

序列表

<110>江苏省人民医院，上海睿昂生物技术有限公司

<120>一种靶向测序试剂盒及其使用方法及靶向测序方法

<141>2018-03-30

<160>2123

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<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>1877

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<212>DNA

<213>人工序列(Artificial sequence)

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<213>人工序列(Artificial sequence)

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<213>人工序列(Artificial sequence)

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<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>1909

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>1910

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>1911

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1911

<210>1912

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1912

<210>1913

<211>29

<212>DNA

<213>人工序列(Artificial sequence)

<400>1913

<210>1914

<211>26

<212>DNA

<213>人工序列(Artificial sequence)

<400>1914

<210>1915

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1915

<210>1916

<211>29

<212>DNA

<213>人工序列(Artificial sequence)

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<210>1917

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

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<210>1918

<211>29

<212>DNA

<213>人工序列(Artificial sequence)

<400>1918

<210>1919

<211>29

<212>DNA

<213>人工序列(Artificial sequence)

<400>1919

<210>1920

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1920

<210>1921

<211>29

<212>DNA

<213>人工序列(Artificial sequence)

<400>1921

<210>1922

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>1922

<210>1923

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1923

<210>1924

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1924

<210>1925

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1925

<210>1926

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>1926

<210>1927

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1927

<210>1928

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1928

<210>1929

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1929

<210>1930

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1930

<210>1931

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1931

<210>1932

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1932

<210>1933

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1933

<210>1934

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>1934

<210>1935

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1935

<210>1936

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>1936

<210>1937

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1937

<210>1938

<211>23

<212>DNA

<213>人工序列(Artificial sequence)

<400>1938

<210>1939

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1939

<210>1940

<211>23

<212>DNA

<213>人工序列(Artificial sequence)

<400>1940

<210>1941

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1941

<210>1942

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>1942

<210>1943

<211>26

<212>DNA

<213>人工序列(Artificial sequence)

<400>1943

<210>1944

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1944

<210>1945

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1945

<210>1946

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1946

<210>1947

<211>21

<212>DNA

<213>人工序列(Artificial sequence)

<400>1947

<210>1948

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1948

<210>1949

<211>21

<212>DNA

<213>人工序列(Artificial sequence)

<400>1949

<210>1950

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1950

<210>1951

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1951

<210>1952

<211>21

<212>DNA

<213>人工序列(Artificial sequence)

<400>1952

<210>1953

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1953

<210>1954

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1954

<210>1955

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1955

<210>1956

<211>19

<212>DNA

<213>人工序列(Artificial sequence)

<400>1956

<210>1957

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1957

<210>1958

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1958

<210>1959

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1959

<210>1960

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>1960

<210>1961

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1961

<210>1962

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1962

<210>1963

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1963

<210>1964

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1964

<210>1965

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1965

<210>1966

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>1966

<210>1967

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>1967

<210>1968

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1968

<210>1969

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1969

<210>1970

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1970

<210>1971

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1971

<210>1972

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1972

<210>1973

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1973

<210>1974

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1974

<210>1975

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1975

<210>1976

<211>323

<212>DNA

<213>人工序列(Artificial sequence)

<400>1976

<210>1977

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1977

<210>1978

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1978

<210>1979

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1979

<210>1980

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1980

<210>1981

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1981

<210>1982

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>1982

<210>1983

<211>21

<212>DNA

<213>人工序列(Artificial sequence)

<400>1983

<210>1984

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1984

<210>1985

<211>23

<212>DNA

<213>人工序列(Artificial sequence)

<400>1985

<210>1986

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1986

<210>1987

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1987

<210>1988

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1988

<210>1989

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1989

<210>1990

<211>26

<212>DNA

<213>人工序列(Artificial sequence)

<400>1990

<210>1991

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>1991

<210>1992

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>1992

<210>1993

<211>26

<212>DNA

<213>人工序列(Artificial sequence)

<400>1993

<210>1994

<211>23

<212>DNA

<213>人工序列(Artificial sequence)

<400>1994

<210>1995

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>1995

<210>1996

<211>23

<212>DNA

<213>人工序列(Artificial sequence)

<400>1996

<210>1997

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>1997

<210>1998

<211>26

<212>DNA

<213>人工序列(Artificial sequence)

<400>1998

<210>1999

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>1999

<210>2000

<211>23

<212>DNA

<213>人工序列(Artificial sequence)

<400>2000

<210>2001

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>2001

<210>2002

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>2002

<210>2003

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>2003

<210>2004

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>2004

<210>2005

<211>29

<212>DNA

<213>人工序列(Artificial sequence)

<400>2005

<210>2006

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2006

<210>2007

<211>29

<212>DNA

<213>人工序列(Artificial sequence)

<400>2007

<210>2008

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>2008

<210>2009

<211>33

<212>DNA

<213>人工序列(Artificial sequence)

<400>2009

<210>2010

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2010

<210>2011

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2011

<210>2012

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2012

<210>2013

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>2013

<210>2014

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2014

<210>2015

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>2015

<210>2016

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2016

<210>2017

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>2017

<210>2018

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2018

<210>2019

<211>20

<212>DNA

<213>人工序列(Artificial sequence)

<400>2019

<210>2020

<211>25

<212>DNA

<213>人工序列(Artificial sequence)

<400>2020

<210>2021

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

<400>2021

<210>2022

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2022

<210>2023

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2023

<210>2024

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>2024

<210>2025

<211>23

<212>DNA

<213>人工序列(Artificial sequence)

<400>2025

<210>2026

<211>27

<212>DNA

<213>人工序列(Artificial sequence)

<400>2026

<210>2027

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<212>DNA

<213>人工序列(Artificial sequence)

<400>2027

<210>2028

<211>24

<212>DNA

<213>人工序列(Artificial sequence)

<400>2028

<210>2029

<211>28

<212>DNA

<213>人工序列(Artificial sequence)

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<210>2030

<211>22

<212>DNA

<213>人工序列(Artificial sequence)

<400>2030

<210>2031

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2032

<211>26

<212>DNA

<213>人工序列(Artificial sequence)

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<210>2033

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2034

<211>21

<212>DNA

<213>人工序列(Artificial sequence)

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<210>2035

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2036

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2037

<211>30

<212>DNA

<213>人工序列(Artificial sequence)

<400>2037

<210>2038

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<212>DNA

<213>人工序列(Artificial sequence)

<400>2038

<210>2039

<211>20

<212>DNA

<213>人工序列(Artificial sequence)

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<210>2040

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2041

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2042

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2043

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2050

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<210>2083

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<211>30

<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

<400>2120

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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<212>DNA

<213>人工序列(Artificial sequence)

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