首页> 中国专利> 柳枝稷木质素合成酶基因PvCCoAOMT全长cDNA及其克隆方法

柳枝稷木质素合成酶基因PvCCoAOMT全长cDNA及其克隆方法

页面导航

摘要
著录项
法律信息
相似文献

摘要

本发明公开了一种柳枝稷木质素合成酶基因PvCCoAOMT的全长cDNA，所述全长cDNA具有（1）或（2）所示的核苷酸序列：（1）SEQ ID No.1所示的核苷酸序列；（2）SEQ ID No.1所示的核苷酸序列经取代、缺失或添加一个或几个核苷酸而形成的具有同等功能的核苷酸序列。本发明还提供了所述柳枝稷木质素合成酶基因PvCCoAOMT全长cDNA的克隆方法。本发明为后续利用柳枝稷木质素合成酶基因PvCCoAOMT全长cDNA序列设计引物、构建载体和遗传转化研究提供了便利，为今后在柳枝稷中开展荧光定量表达、转基因、基因功能验证等研究奠定了基础。

著录项

公开/公告号CN103343133A

专利类型发明专利
公开/公告日2013-10-09

原文格式PDF
申请/专利权人中国农业大学;
展开▼

申请/专利号CN201310277693.4
发明设计人张蕴薇;刘斯佳;杨富裕;方程;李洪超;黄艳华;杜娟;
展开▼

申请日2013-07-03
分类号C12N15/54(20060101);C12N9/10(20060101);C12N15/10(20060101);C12N15/70(20060101);C12N1/21(20060101);C12R1/19(20060101);
代理机构11002 北京路浩知识产权代理有限公司;
代理人王朋飞
地址 100193 北京市海淀区圆明园西路2号
入库时间 2024-02-19 19:50:28

法律信息

法律状态公告日

法律状态信息

法律状态
2015-12-02

授权

授权
2013-11-06

实质审查的生效 IPC(主分类):C12N15/54 申请日:20130703

实质审查的生效
2013-10-09

公开

公开

相似文献

专利
中文文献
外文文献

1. 柳枝稷木质素合成酶基因PvCCoAOMT全长cDNA及其克隆方法 [P] . 中国专利： CN103343133B . 2015.12.02
2. 柳枝稷木质素合成酶基因PvCCoAOMT全长cDNA及其克隆方法 [P] . 中国专利： CN103343133A . 2013-10-09
3. Genetically engineered hepatitis c virus (hcv) nucleic acid clone, plasmid clone that houses a life-size hcv cdna, dna or rna transcribed from the full-length hcv cdna, transfected and in vitro infected host cell line with hcv, non-human animal infected with hcv, hcv viral particle, in vitro test system, hcv cell free, hcv vaccine and hcv test set and processes for identifying a cell line that is permissive and an animal that is permissive for hcv infection, to select hcv with adaptive mutations that allow higher levels of hcv replication in a permissive cell line, to infect an animal with hcv, to spread hcv in vitro, to transduce an animal susceptible to hcv infection with a heterologous gene, to produce hcv viral particles, hcv particle proteins and hcv antibodies, to separate agents capable of to modulate hcv replication, to prepare a hcv nucleic acid and to detect hcv antibodies in a biological sample from a patient. [P] . BR9808147A . 2000-03-28

机译：基因工程丙型肝炎病毒（hcv）核酸克隆，质粒克隆，其中包含从全长hcv cdna转录而来的真人大小的hcv cdna，dna或rna，并通过非人类动物的hcv转染和体外感染宿主细胞系被hcv感染，hcv病毒颗粒，体外测试系统，无hcv细胞，hcv疫苗和hcv测试仪的感染以及鉴定允许的细胞系和允许感染hvv的动物的过程，以选择具有以下适应性突变的hcv：允许在允许的细胞系中进行更高水平的hvv复制，以hvv感染动物，在体外传播hvv，用异源基因转导易感染hvv的动物，产生hvv病毒颗粒，hvv颗粒蛋白和hvv抗体分离能够调节hcv复制的试剂，制备hcv核酸并检测患者生物样品中的hcv抗体。
4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途