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利用细菌核酸指纹特征谱库进行鉴定和分类的用途

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摘要

本发明公开了一种用于鉴定和分类细菌的核酸指纹图谱库及其制备方法，包括PCR扩增、SAP酶消化、转录和核酸酶切、纯化、质谱仪检测等步骤。基于该方法，建立常见细菌的核酸指纹图谱数据库。根据实验产生的质谱特征图谱库，可对待检细菌进行分类与鉴定，结果可广泛运用于细菌分类和鉴定、遗传进化分析、耐药筛选用途以及进出口检验等领域。

著录项

公开/公告号CN102851747A

专利类型发明专利
公开/公告日2013-01-02

原文格式PDF
申请/专利权人向华;
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申请/专利号CN201210273570.9
发明设计人马庆伟;赵洪斌;张海燕;
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申请日2012-08-02
分类号C40B50/06(20060101);C40B40/06(20060101);C12Q1/68(20060101);C12Q1/04(20060101);C12N15/10(20060101);
代理机构
代理人
地址 410128 湖南省长沙市芙蓉区农大路1号湖南农业大学兴湘楼405室
入库时间 2024-02-19 16:29:48

法律信息

法律状态公告日

法律状态信息

法律状态
2017-12-26

专利权人的姓名或者名称、地址的变更 IPC(主分类):C40B50/06 变更前: 变更后: 申请日:20120802

专利权人的姓名或者名称、地址的变更
2016-06-15

著录事项变更 IPC(主分类):C40B50/06 变更前: 变更后: 申请日:20120802

著录事项变更
2014-04-30

授权

授权
2014-03-26

专利申请权的转移 IPC(主分类):C40B50/06 变更前: 变更后: 登记生效日:20140307 申请日:20120802

专利申请权、专利权的转移
2014-03-26

著录事项变更 IPC(主分类):C40B50/06 变更前: 变更后: 申请日:20120802

著录事项变更
2013-04-24

实质审查的生效 IPC(主分类):C40B50/06 申请日:20120802

实质审查的生效
2013-01-02

公开

公开

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1. 利用细菌核酸指纹特征谱库进行鉴定和分类的用途 [P] . 中国专利： CN102851747A . 2013-01-02
2. 制备细菌核酸指纹特征谱库的方法 [P] . 中国专利： CN103060925A . 2013-04-24
3. Nucleic acid sequence, amino acid sequence, replicative cloning vector, isolated host cell expression vector, methods for testing a substance as a therapeutic agent for bone modulation in a host to identify a molecule and a candidate protein involved in bone modulation, to test for hbm activity, for pharmaceutical development to treat bone development disorders, to treat a bone development disorder in an animal, to alter bone development in a host, to treat osteoporosis, to evaluate diagnosis for a genetic predisposition to a bone development disorder, to identify a genetic predisposition to bone development disorders, to express hbm protein in bone tissue, to amplify a nucleotide polymorphism, to identify a regulatory element of an hbm gene, and to modulate bone density and in one patient, diagnostic assay for bone development disorders, bacterial artificial chromosome, isolated nucleic acid segment, nucleic acid, and polypeptide. [P] . BR0017197A . 2003-01-14

机译：核酸序列，氨基酸序列，复制性克隆载体，分离的宿主细胞表达载体，在宿主中测试作为骨调节治疗剂的物质的方法以鉴定参与骨调节的分子和候选蛋白以测试hbm活性，用于药物开发以治疗骨骼发育异常，治疗动物骨骼发育异常，改变宿主的骨骼发育，治疗骨质疏松症，评估对骨骼发育异常的遗传易感性诊断，鉴定遗传易患骨发育障碍，在骨组织中表达hbm蛋白，扩增核苷酸多态性，鉴定hbm基因的调节元件并调节骨密度，并且在一名患者中进行骨发育障碍的诊断测定，细菌人工染色体，分离的核酸片段，核酸和多肽。
4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途
5. Process for the uptake, accumulation and / or use of nitrogen increased and / or the content of total nitrogen increased in a photosynthetic organism active molecule of nucleic acid isolation, construction of nucleic acid, vector, host cell, a process for producing a Polipeptu00ecdeo, polipeptu00ecdeo, antibody, plant tissue, propagating material, harvested material or a plant.Method for the identification of a gene product, composition, and use of the molecule of nucleic acid, the polipeptu00ecdeo, construction of nucleic acid, the vector of the plant or plant tissue, harvested material of the host cell or gene product identified in accordance with the baa All [P] . BRPI0713097A2 . 2012-10-16

机译：分离核酸的光合生物活性分子中氮的吸收，积累和/或利用的过程和/或总氮的增加的过程，核酸的构建，载体，宿主细胞，多肽，抗体，植物组织，繁殖材料，收获的材料或植物。鉴定核酸分子的基因产物，组成和用途的方法，多肽，核酸的构建酸，根据baa鉴定的植物或植物组织的载体，宿主细胞的收获材料或基因产物