应用ELISA 定量检测转基因玉米中

摘要

[中文文摘]应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。[英文文摘]Basedontheantibody-antigen-enzymeantibodyreaction andbyusingpuriftiedBt1insecticidalcrystalproteinasboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was establishedto detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, In comparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISAmethodmightbeusefulforeasy,rapid,reliableandeffectiveassayinlargesamples.