［中文文摘］以阳离子花菁染料为荧光探针， 依近红外荧光猝灭测定核酸． 最大激发及发射波长分别为７６５ 、７９０ｎｍ ． 核酸测定的线性范围为０－０８ ～１－２μｇ／ｍ Ｌ（ＣＴＤＮＡ 及ＳＭ ＤＮＡ） ， ０－１ ～１－６μｇ／ｍＬ（yeａｓｔ ＲＮＡ)．［英文文摘］A method with an cationic near-IR cyanine as a fluorescent probe was developed for the determination of nucleic acids.The near-IR cyanine shows the maximum excitation and emission wavelengths at 765 and 790nm, respectively,in aqueous solution.The method is based on the fluorescence decrease of near-IR cyanine in the presence of nucleic acids.Under optimal conditions,the ratio value of fluorescence intensity in the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range of 0108～112μg/ mL for CT DNA or SM DNA , and 011～116μg/ mL for Yeast RNA. The detection limit s were 30 ng/ mL for CT DNA , 25 ng/ mL for SM DNA and 70 ng/ mL for Yeast RNA. The relative standard deviation (n = 6) were 211%for 500 ng/ mL CT DNA level.
展开▼
机译：［中文文摘］以阳离子花菁染料为荧光探针， 依近红外荧光猝灭测定核酸． 最大激发及发射波长分别为７６５ 、７９０ｎｍ ． 核酸测定的线性范围为０－０８ ～１－２μｇ／ｍ Ｌ（ＣＴＤＮＡ 及ＳＭ ＤＮＡ） ， ０－１ ～１－６μｇ／ｍＬ（yeａｓｔ ＲＮＡ)．［英文文摘］A method with an cationic near-IR cyanine as a fluorescent probe was developed for the determination of nucleic acids.The near-IR cyanine shows the maximum excitation and emission wavelengths at 765 and 790nm, respectively,in aqueous solution.The method is based on the fluorescence decrease of near-IR cyanine in the presence of nucleic acids.Under optimal conditions,the ratio value of fluorescence intensity in the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range of 0108～112μg/ mL for CT DNA or SM DNA , and 011～116μg/ mL for Yeast RNA. The detection limit s were 30 ng/ mL for CT DNA , 25 ng/ mL for SM DNA and 70 ng/ mL for Yeast RNA. The relative standard deviation (n = 6) were 211%for 500 ng/ mL CT DNA level.
展开▼
