采用活体注射和体外培养方法 ,进行生物胺调控雌性锯缘青蟹生殖神经内分泌的研究。结果表明 ,锯缘青蟹于实验的第 1天 ,第 5天 ,第 1 0天 ,分别注射 1 0 0 μl剂量为 1 μg/g体重的 5羟色胺 (5 HT)、章鱼胺 (OA)和多巴胺 (DA) ,第 1 5天解剖 ;其中 5 HT对卵巢发育具有显著的促进作用 ,OA和DA的作用不明显。离体条件下 ,将脑、胸神经团、视神经节分别与卵巢小块进行联合培养 ,每个实验组分别加入PBS与 1 μg/g体重的5 HT、OA、DA、HA(组胺 )和NE(去甲肾上腺素 ) ,发现卵巢发育的促进作用与 5 HT刺激了脑和胸神经团的分泌活动有关 【英文摘要】 The possibility that biogenic amines affect ovarian development in the female mud crab, Scylla serrata, was investigated. Females were administered 1μg/g body weight of 5-hydroxytryptamine (5-HT)?octopamine (OA) or dopamine (DA) on days 1, 5 and 10 and were sacrificed on day 15. Crabs given 5-HT showed significant increases in ovarian index and oocyte size over the concurrent controls, while OA and DA did not significantly affect either the ovarian index or oocyte size. In vitro study indicates that none ...
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机译：采用活体注射和体外培养方法 ,进行生物胺调控雌性锯缘青蟹生殖神经内分泌的研究。结果表明,锯缘青蟹于实验的第1天,第5天,第1 0天,分别注射1 0 0 μl剂量为1 μg/g体重的5羟色胺(5 HT)、章鱼胺(OA)和多巴胺(DA) ,第1 5天解剖;其中5 HT对卵巢发育具有显着的促进作用,OA和DA的作用不明显。离体条件下,将脑、胸神经团、视神经节分别与卵巢小块进行联合培养,每个实验组分别加入PBS与1 μg/g体重的5 HT、OA、DA、HA(组胺)和NE(去甲肾上腺素) ,发现卵巢发育的促进作用与5 HT刺激了脑和胸神经团的分泌活动有关【英文摘要】 The possibility that biogenic amines affect ovarian development in the female mud crab, Scylla serrata, was investigated. Females were administered 1μg/g body weight of 5-hydroxytryptamine (5-HT)?octopamine (OA) or dopamine (DA) on days 1, 5 and 10 and were sacrificed on day 15. Crabs given 5-HT showed significant increases in ovarian index and oocyte size over the concurrent controls, while OA and DA did not significantly affect either the ovarian index or oocyte size. In vitro study indicates that none ...
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