Analysis of effects of Omega-3, -6 and -9 fatty acids on expression of ErK protein relating to MAPK signaling pathway in SK-OV-3 cell line, relating to ovarian cancer

摘要

Despite recent progress in cancer treatment, cancer still remains as a major public health problem in many parts of the world. Epithelial ovarian cancer is one of the most mortal and treat-resistant cancers among gynecological cancers. A novel approach to chemotherapy has the potential to yield novel dietary-drug, dietary-dietary and/or drug-drug combinations that can provide additive or even synergistic protection against the progression of cancers. There is considerable evidence that unsaturated fatty acids, in addition to their role as an energy source, might affect both cancer development and progression.udIn this study we investigated cytotoxic effects of n-3, n-6 and n-9 unsaturated fatty acids on SKOV-3 (human ovarian adenocarcinoma) cell line by MTT assay. Using an Annexin-V-FLOUS staining kit, we evaluated apoptosis induction in these cells. Finally, we performed western blotting for ErK and phospho-ErK in order to investigate the impact of unsaturated fatty acids on MAPK signaling pathway.udThe MTT results showed that Linoleic acid (LA, n-6) and Oleic acid (OA, n-9) at concentrations lower than 500 µM promoted cell proliferation and were cytotoxic at higher concentrations. On the other hand, Alpha-linolenic (ALA, n-3) acid and Arachidonic acid (AA, n-6) had no effect on SKOV-3 cell proliferation at concentrations used. Also, it has been clarified cytotoxic effects of LA and OA is due to their ability to induce apoptosis. Following western blotting it has been determined that LA and OA reduced ErK activation.