In vivo generation of 'vaccination nodes' using injectable alginate hydrogels for cancer immunotherapy

摘要

Despite the amount of ongoing intensive research, tumor cells have continued to outwit us in the effort to combat and prevent cancer by exerting a number of mechanisms to evade and suppress anti-tumor immune responses. The present work employs strategies to generate a synthetic extranodal immunoplatform that can harbor both exogenously provided and endogenously recruited immune cells (primed against tumor cells), at the same time providing immuno-factors that support these cells and counter immunosuppressive effects from tumors. We have developed injectable self-gelling alginate formulations for this purpose, enabling sustained release of soluble immunomodulatory factors from the gels and presentation of immobilized immunostimulatory factors inside the gels. The hydrogels injected into the back flanks of mice formed a macroporous structure that allowed easy cell infiltration and migration. Modulation of the mechanical properties of self-gelling alginate was possible by varying the number of calcium-bound microspheres in the gels. During characterization of immune responses using these hydrogels, alginate gels carrying activated dendritic cells (DCs) were shown to dramatically increase the number of T cells recruited to the local injection site. When the dendritic cells were pulsed with antigen, these 'vaccination nodes' were able to initiate an antigen-specific immune response, with some of the injected DCs migrating to the regional lymph nodes and priming cognate T cells. The activated antigen-specific T cells then migrated to the injection site and infiltrated the gels, causing an effector re-trafficking phenomenon that guided both T cells and host dendritic cells to the gels.