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Multichannel microfluidic device to dynamically control oxygen concentration conditions in-vitro

机译:多通道微流体装置,用于在体外动态控制氧浓度条件

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摘要

Multilayer microfluidic devices were designed and fabricated such that an array of different oxygen concentrations could be applied to a testing area in any desired sequence and with unconstraint application times. The principle of flow resistance dictates that a large channel length will impose a larger resistance and therefore a larger reduction in flow rate versus a shorter channel length. To exploit this feature, the microfluidic device employs a fluidic resistance network composed of an array of predetermined variable length channels to generate different oxygen to nitrogen flow rate ratios, i.e. different oxygen concentrations. Standard lithographic techniques were used to fabricate the microfluidic devices, using highly gas permeable silicone rubber (polydimethylsiloxane (PDMS)). The stacked microchannel architecture, channel dimensions, and layer thicknesses in the device were optimized for rapid diffusion and saturation of 02 N2 mixtures into the testing areas. The oxygen concentration was dynamically monitored using polymeric fluorescence-based oxygen sensors integrated into the device. By altering oxygen levels over time, this device aims to selectively build up biofilms on the artificial tooth substrate as the process occurs in-vitro. A study concerning this application is also presented.
机译:设计和制造多层微流体装置,以便可以以任何所需顺序和不受限制的施加时间将不同氧气浓度的阵列施加到测试区域。流动阻力的原理表明,较大的通道长度将施加较大的阻力,因此与较短的通道长度相比,流速的减小幅度更大。为了利用此特征,微流体装置采用由预定可变长度通道的阵列组成的流体阻力网络,以产生不同的氧气与氮气的流量比,即不同的氧气浓度。使用高透气性的硅橡胶(聚二甲基硅氧烷(PDMS)),使用标准的光刻技术来制造微流体装置。优化了设备中的堆叠式微通道架构,通道尺寸和层厚度,以使02 N2混合物快速扩散和饱和进入测试区域。使用集成到设备中的基于聚合物荧光的氧气传感器动态监测氧气浓度。通过随着时间改变氧气水平,该设备旨在在体外进行过程中在人造牙齿基质上选择性地形成生物膜。还介绍了有关此应用程序的研究。

著录项

  • 作者

    Rodriguez Rosa H;

  • 作者单位
  • 年度 2008
  • 总页数
  • 原文格式 PDF
  • 正文语种 eng
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