Isolation of Corn Seed Hemicellulose-Decomposing Bacteria Strain (No.101) from Corn Seed and preparation of crude Hemicellulase.

摘要

Using a medium containing inorganic nitrogen sauce and corn seed hemicellulose only carbon sauce as a screening culture medium, we isolated a strong corn seed hemicellulose decomposing strain of wild lactic acid bacteria like from a corn seed. For the enzyme formation three times volum of acetone was added to the filtrate (2L) of culture medium. A small amount of white wasted precipitate was formed. This precipitate was centrifuged and freez-dried. About 300mg crude red-yellow hemicellulase powder was obtained. An experiment on the relationship between the various concentrations of hemicellulase and viscosity of corn seed hemicellulose solutiou showed that the viscosity drawing rateincreases to some extent with increase in enzyme concentration. The optimnm ph of the enzyme action lies at about 6.0.