首页> 外文OA文献 >Molecular Cloning of cDNAs Encoding Pituitary Glycoprotein Hormone .ALPHA., FSH .BETA. and LH .BETA. Subunits in Ayu, Plecoglossus altivelis.
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Molecular Cloning of cDNAs Encoding Pituitary Glycoprotein Hormone .ALPHA., FSH .BETA. and LH .BETA. Subunits in Ayu, Plecoglossus altivelis.

机译:分子克隆编码垂体糖蛋白激素.aLpHa。,FsH .BETa。和LH .BETa。亚基,ayu,plecoglossus altivelis。

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摘要

Complementary DNAs (cDNAs) encoding follicle-stimulating hormone (FSH)β, luteinizing hormone (LH)β and glycoprotein hormone (GPH) a subunits were isolated and characterized from ayu pituitary using a PCR technique. The FSHβ, LHβ and GPHα subunit cDNAs were found to be 556, 588 and 621 base pair (bp) long, encoding 384, 432 and 357bp long open reading frames, respectively. The deduced amino acid sequences of the putative mature forms of ayu GPHα and LHβ subunits were on average 63% homologous with those of other teleosts, whereas ayu FSHβ subunit was on average 42% homologous. These results show that ayu has two different types of gonadotropins (FSH and LH), as in other teleosts. FSHβ subunit mRNA was mainly detected during early vitellogenesis and spermatogenesis. In contrast, LHβ subunit mRNA was detected during the late phase of gonadal development, suggesting that the primary function of FSH may be to initiate gametogenesis, while LH may have a role in the development of gametes.
机译:使用PCR技术从垂体垂体中分离出编码促卵泡激素(FSH)β,促黄体生成素(LH)β和糖蛋白激素(GPH)a亚基的互补DNA(cDNA)。发现FSHβ,LHβ和GPHα亚基cDNA长556、588和621个碱基对(bp),分别编码384、432和357bp长的开放阅读框。推定的ayuGPHα和LHβ亚基成熟形式的推导氨基酸序列与其他硬骨鱼的同源性平均为63%,而ayuFSHβ亚基的同源性平均为42%。这些结果表明,与其他硬骨鱼一样,阿玉具有两种不同类型的促性腺激素(FSH和LH)。 FSHβ亚基mRNA主要在早期卵黄发生和精子发生期间检测到。相反,在性腺发育的后期检测到LHβ亚基mRNA,这表明FSH的主要功能可能是启动配子发生,而LH可能在配子的发育中起作用。

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