Determination of Calcium by Cheletemetry Using Calcein―II. Determination of the Total Calcium and the Dialysate Calcium in Blood Serum

摘要

For the estimation of the blood serum calcium, the YANAGISAWA method of colorimetry, and the chelate titration method with EDTA 2Na using Eriochrome Black T and Murexide as the indicators are generally used, but these methods are found to have some defects. The authors reported in a previous paper an improved method of the chelate titration in which they used calcein, a kind of chelate indicator, to determine the conditions of estimation of calcium in drinking water and sea water. In our present experiment, this method has been employed for the estimation of total blood serum calcium and dialysate calcium, and it brought about a very satisfactory result. The method proved to be the same as the YANAGISAWA method in accuracy and by far more advantageous than that in its simple experimental operation and in the shortening of the time needed.