Photosynthetic Pigments of the Different Color Types of Porphyra yezoensis UEDA and P. tenera KJELLMAN

摘要

The thalli of red (two types, Ⅰ and Ⅱ), orange, green, yellow and wild types of Porphyra yezoensis UEDA and green type of P. tenera KJELLMAN were simultaneously cultured in the Ariake Sea for the same period, and were analyzed for photosynthetic pigments. Chlorophyll a, carotenoids, phycoerythrin and phycocyanin were extracted and their contents were spectrophotometrically determined. Phycoerythrin (PE) and phycocyanin (PC) content differed significantly among the specimens, but chlorophyll a and carotenoids levels were about the same. The ratios of PE/PC observed in all type of thalli of P. yezoensis were, by colors in descending order, orangered Ired Ⅱwild yellowgreen. The same order of ratios was also observed among Conchocelis-thalli cultured in the laboratory under similar conditions. On the other hand, the green type of P. tenera resembled closely the green type of P. yezoensis in phycoerythrin and phycocyanin content of Conchocelis-thalli, but the former type was different from the latter type in those contents of the thalli. Phycoerythrin, phycocyanin and allophycocyanin were isolated from each type of thalli by means of ammonium sulfate precipitation and column chromatography on Sephadex G-100 and on calcium phosphate gels. As a result, red Ⅰ, green and yellow types of P. yezoensis differed from the wild type in phycoerythrin and/or phycocyanin types, but phycoerythrin and phycocyanin types of red Ⅱ and orange types of P. yezoensis and green type of P. tenera were the same as those of the wild type. Significant differences in absorption spectrra of allophycocyanin among all specimens were not observed.