Detection of Clostridium perfringens in Sea Mud of Ariake Sea―I A New Method for Detection of Clostridium perfringens in Sea-Mud Specimens

摘要

A new medium (KNP medium) was prepared to detect Clostridium perfringens in sea-mud specimens, and a new method for detection of the organisms was established using the new medium. KNP medium was prepared by modifying LAS medium as described below. i ) Kanamycin was added in addition to neomycin and polymyxin which were already in LAS medium. ii) The pH (7.0 to 7.1) was altered to 6.0, and iii) 0.2g of dried ground meat was added to 10ml of the medium in order to elongate the viable period of the cultures. The method for preparation of KNP medium is described in the footnote of Fig.2. The method for detection of C. perfringens in sea-mud specimens using KNP medium is shown in Fig.2. The average detection rate of C. perfringens in the KNP-positive cultures was 68%. Of the isolates which were positive in both KNP enrichment and egg yolk CW agar isolate cultures, 92% was C. perfringens. KNP medium was applicable to unheated specimens, but not to heated (100℃, 15min.) specimens.