Freeze-Preservation of Germinated Conchospores of Porphyra yezoensis in Viable State

摘要

From the previous investigations, it was demonstrated that the leafy thalli of Porphyra show high frost-resistance. Recently, the freeze-preservation of laver-nets growing the thalli has been put into practical use for Porphyra cultivation in Japan. In this paper, we deal with the frost-resistance of germinated conchospores of Porphyra yezoensis in an attempt to develope a new application of Porphyra cultivation. The results are summarized as follows: 1. The germinated conchospores showed higher resistivity against the freeze-preservation than the free conchospores, but they showed slightly lower resistivity than the germinated monospores. 2. In the freeze-preservation at about -10℃ and -20℃, most of germinated conchospores survived without remarkable damage at least for two weeks. On the other hand, the survival-rate gradually decreased after 25 days storage. 3. In the room-seeding of Porphyra, the freeze-preservation of laver-nets, on which conchospores germinated, will be a useful method of Porphyra cultivation.