White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections

摘要

White spot syndrome virus (WSSV) infection was induced in tiger shrimp, Penaeusudmonodon, under laboratory conditions, and histopathological changes in subcuticular epithelialudcells of the eye stalk and pleopod were studied sequentially at different time post-challenge. Routineudhistological techniques using paraffin embedded tissues, as well as frozen tissues, were used touddocument WSSV infection. Histological manifestations such as cellular hypertrophy in the subcuticularudepithelial cells of the eyestalk and pleopod could be detected as early as 18 h postinfectionud(p.i.) before the manifestation of clinical signs of the disease