Development of a Cell Culture System From Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider)

摘要

A cell culture system was developed successfully from gill explants of the Malabar grouper,udEpinephelus malabaricus. Gill tissue samples aseptically excised from healthy juveniles ofudE. malabaricus were explanted in Leibovitz’ L-15 medium supplemented with 0.07 M NaCl andud20% fetal bovine serum (FBS). A mixture of different types of cells emerged from the explants,udand these cells were observed to spread and attach to the culture flasks from the second dayudonwards. Confluent monolayers comprising epithelioid as well as fibroblast-like cells were formedudwithin ten days. The cells were found to grow well at 28 + 2°C. The cell monolayers wereudsubcultured by trypsinization and seeded into new flasks, which produced confluent monolayersudcomprising predominantly epithelioid-like cells in subsequent passages.