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Changes in bacterial flora during short-term storage of aquafeed and feed ingredients

机译:短期储存水产饲料和饲料原料时细菌菌群的变化

摘要

Bacteriological changes in two shrimp feeds and protein rich ingredients on short-term storage for 60 days were studied. Feed I was a commercial shrimp 'grower' diet and Feed 11, a cost effective diet for shrimp formulated at CMFRI, Cochin. The protein-rich feed ingredients usedudwere fishmeal, groundnut oil cake and soybean flour. Sealed samples were stored under identical conditions at room temperature and were analyzed for bacterial population at 0, 30 and 60 days. The total plate count of feeds and feed ingredients were well within the reported non-degrading limit. Gram-positive cocci (42.8-73%) were dominant in all the five samples. During storage gram-positive cocci showed slight reduction, while, gram-negative rods showedudmarginal increase. Five hundred bacterial isolates derived from feed and feed ingredients were used for further characterization and identification. The major genera identified were Micrococcus, Arthrobacter and Bacillus. Besides, enumeration of indicator bacteria such as Vibrio,udFaecal streptococci, Escherichia coli and Staphylococcus aureus was also done in the feeds and feed ingredients. Feed I1 was devoid of all the above indicator forms, except Vibrio, while, Feed I had all the above groups in varying numbers. Short-term storage (up to 60 d) did not haveudmuch effect on bacterial load in feeds and feed ingredients.
机译:研究了两种虾饲料和富含蛋白质的成分在短期储存60天后的细菌学变化。饲料I是一种商业虾“生长”饲料,而饲料11是在Cochin的CMFRI配制的具有成本效益的虾饲料。所用的富含蛋白质的饲料原料是鱼粉,花生油饼和大豆粉。密封的样品在室温下在相同条件下存储,并在0、30和60天分析细菌种群。饲料和饲料成分的总板数完全在报告的非降解限度内。革兰氏阳性球菌(42.8-73%)在所有五个样本中占主导地位。在贮藏期间,革兰氏阳性球菌显示略有减少,而革兰氏阴性球菌显示 udmarginal增加。来自饲料和饲料成分的五百种细菌分离物用于进一步表征和鉴定。确定的主要属是微球菌,节杆菌和芽孢杆菌。此外,在饲料和饲料成分中还进行了指示性细菌的计数,例如弧菌,udFaecal链球菌,大肠埃希氏菌和金黄色葡萄球菌。资讯提供I1除弧菌外没有上述所有指示剂形式,而资讯提供I具有上述所有不同数量的组。短期储存(长达60天)对饲料和饲料成分中的细菌负荷没有太大影响。

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