Surface Enhanced Raman Spectroscopy detection of p-coumaric acid from cell supernatant using gold-capped silicon nanopillar substrates

摘要

The purpose of the project is to use Surface Enhanced Raman Spectroscopy (SERS) to discriminate between two different bacterialpopulations, based on their p-coumaric acid (pHCA) production. The pHCA concentration is measured in a droplet of diluted supernatantdried on SERS substrates, using a Raman microscope. By analyzing the SERS signal of pHCA from the supernatant, considering the peakheight at the characteristic frequency (1169 cm-1) it is possible to distinguish between a producing and control strain, as also confirmedby HPLC analysis.