Rapid sand filtration is a traditional and common technology for drinking water purification from groundwater. Despite its wide scale and long-term use, the diversity and characterization of microbial communities in these engineered systems have remained unexplored and their roles in removal performances yet to be discovered. In order to explore the microbial ecology of these systems, we conducted 16S rRNA gene (rDNA) based 454 pyrosequencing as a deep sequencing approach to 94 sample cores retrieved from 5 different waterworks including proper biological replication. This comprehensive sampling of replicate rapid sand filters across many waterworks together with high-throughput sequencing provides a first glimpse into the microbial communities in rapid sand filters and their potential roles in the treatment process.
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