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A vibrating membrane bioreactor operated at supra- and sub-critical flux: Influence of extracellular polymeric substances from yeast cells

机译:振动膜生物反应器在超临界和亚临界通量下操作:来自酵母细胞的细胞外聚合物质的影响

摘要

A vibrating membrane bioreactor, in which the fouling problems are reduced by vibrating a hollow fiber membrane module, has been tested in constant flux microfiltration above (supra-critical) and below (sub-critical) an experimentally determined critical flux. Suspensions of bakers yeast cells were chosen as filtration medium (dry weight 4 g/l). The influence of extracellular polymeric substances (EPS) from the yeast cells is evaluated by UV absorbance measurements of the bulk supernatant during filtration. The critical flux seems to be an interval or a relative value rather than an absolute value. Filtration just below the critical flux (sub-critical) seems to be a good compromise between acceptable flux level and acceptable increase of fouling resistance and trans-membrane pressure (TMP) in a given time period. EPS from the yeast cells causes the membrane module to foul and part of the fouling is irreversible. A fraction of the EPS content is loosely bounded in the yeast cells and is easily and fast washed out when suspended in water. Another fraction of the EPS content is more tightly bound in the yeast cells and is therefore not washed out as easily. A part of this tightly bounded EPS content is continually washed out during supra-critical flux operation whereas the washing out at sub-critical flux operation is not observed. This might be due to locally different hydrodynamic conditions at the membrane surface and pore entrances at supra- and sub-critical flux respectively.
机译:一种振动膜生物反应器,其中通过振动中空纤维膜组件减少了结垢问题,已经在高于(超临界)和低于(亚临界)实验确定的临界通量的恒定通量微滤中进行了测试。选择面包酵母细胞的悬浮液作为过滤介质(干重4 g / l)。通过过滤过程中大量上清液的紫外线吸收率测量来评估来自酵母细胞的细胞外聚合物质(EPS)的影响。临界通量似乎是一个间隔或一个相对值,而不是绝对值。在给定时间段内,过滤在临界通量以下(次临界)似乎是可接受的通量水平与可接受的防污性和跨膜压力(TMP)的增加之间的良好折衷。来自酵母细胞的EPS会导致膜组件结垢,并且部分结垢是不可逆的。 EPS含量的一小部分松散地结合在酵母细胞中,当悬浮在水中时,可以轻松快速地洗掉。 EPS含量的另一部分更紧密地结合在酵母细胞中,因此不容易被洗掉。在超临界通量操作期间,该紧密结合的EPS含量的一部分会被连续冲洗掉,而在亚临界通量操作下则没有观察到冲洗掉。这可能是由于分别在超临界和次临界通量下在膜表面和孔入口处局部不同的流体动力学条件。

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