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Axial Resolution of Confocal Raman Microscopes: Gaussian Beam Theory and Practice
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机译:共焦拉曼显微镜的轴向分辨率:高斯光束理论与实践
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摘要
A straightforward and transparent model, based on Gaussian beam optics, for the axial r0 resolution of a confocal microscope is presented. A confocal Raman microscope was used to determine the axial confocality in practice. The axial response of a thin planar object was measured for three different objectives, two pinhole sizes and a slit. The results show that, in the case of a confocal configuration, the response calculated with the model provides a good prediction of the axial resolution of the confocal microscope.
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