Dependence of protein mechanical unfolding pathways on pulling speeds

摘要

Mechanical unfolding of the fourth domain of Distyostelium discoideum filamin(DDFLN4) was studied in detail using the C$_{\alpha}$-Go model. We show thatunfolding pathways of this protein depend on the pulling speed. The agreementbetween theoretical and experimental results on the sequencing of unfoldingevents is achieved at low loading rates. The unfolding free energy landscape isalso constructed using dependencies of unfolding forces on pulling speeds.