共同轉殖蘇力菌殺蟲結晶蛋白、熱休克蛋白、超氧化歧化酵素及過氧化氫酵素基因至甘藍之研究

摘要

Attempts had been made to co-transfer the Bt-toxin gene (bt), superoxide dismutase gene (sod62) of Chinese cabbage, catalase gene (cat78) of Chinese cabbage, and heat shock protein gene (hsc70) of tomato into the cabbage (Brassica oleracea L. var. K-Y cross). The objectivesof this study are to establish the technology of gene co-transferand targeting the foreign proteins into the chloroplast by chloroplast trainsit peptide, and to study the possibility for improvement of cabbage with insect, disease, and stresses resistance by genes co-trans-formation. udThe results of PCR, Southern nad Northern hybridization indicated that the bt, hsc70, sod62 and cat78 genes had been inserted into the genome of cabbage, and expressed. The result of isozyme profiles assay revealed that the transformed SOD62 and CAT78 were present in the transformed plants. The SOD and CAT activities in leaves and chloroplast of transformed plants were higher than those of the controls. Increases in amounts of HSC70 protein were found in the transformed plants s analyzing by western blot hybridization.udPreliminary results of bioassay indicated that the increases in tolerance to high temperature (42℃, 4 days), SO2 (800 ppb, 3 days), and insect resistance (Plutella xylostella) were found in the transgenic plants. This study demonstrated that two, three and four foreign genes could be co-transgenic plants. This study demonstrated that two, three and four foreign genes could be co-transferred into a cabbage plant simultaneously via Agrobacterium mediated transformation.