A taqman-based method to screen cucumber cultivars for resistance to Cucumber Vein Yellowing Virus

摘要

Cucumber vein yellowing virus (CVYV), which is included in the EPPO Alert List of pathogens, has become a limiting factor of cucumber production in the southeast of Spain. This has forced the crop producers to optimize preventive control measures such as the use of pest-free seedlings, adequate greenhouse-window screens, double doors and the treatment of infected vegetable residues. In addition, the use of new commercial cucumber cultivars with resistance to CVYV has become widespread in Spain. However, from our field studies, as well as from the general opinion of farmers and technicians, we suspected that this resistance to CVYV in the different cultivars did not always match with what was proclaimed by the seed companies. In this work we compare the evolution of CVYV after inoculation in different cucumber cultivars during two different crop seasons to optimize a tool that enables farmers to apply evidence-based crop management. The method described in this paper is based upon real-time RT-PCR (TaqMan® chemistry) assay. An internal control has been used to avoid false positives and to normalize the results.